Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,300,860 T→C 7.9% D16G (GAT→GGT) ‡ lpxH ← UDP‑2,3‑diacylglucosamine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,300,8600TC 7.9% 216.4 / 7.7 76D16G (GAT→GGT) ‡lpxHUDP‑2,3‑diacylglucosamine hydrolase
Reads supporting (aligned to +/- strand):  ref base T (33/37);  new base C (4/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGACCACTTCA  >  NC_002947/3300715‑3301008
                                                                                                                                                 |                                                                                                                                                      
ccGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCg                                                                                                                                                     >  2:274875/1‑149 (MQ=255)
       aTGGCCTGGCAGATCGAGTGCTGGCAGGGCGGCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCACGGATGTACAGGGCCTTGGCTTGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGATATTTCCCGGGCGttt                                                                                                                                              <  6:209747/149‑2 (MQ=255)
       aTGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                                              <  5:289217/149‑1 (MQ=255)
            cTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTTGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                                              >  6:366057/1‑144 (MQ=255)
            cTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTTGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGttc                                                                                                                                              <  5:366057/144‑1 (MQ=255)
             tGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                                        >  2:169935/1‑149 (MQ=255)
               gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                                        >  1:115866/1‑147 (MQ=255)
               gCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTg                                                                                                                                        <  6:201449/147‑1 (MQ=255)
                  gATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGt                                                                                                                                 >  2:305854/1‑149 (MQ=255)
                   aTCGACTGCTGGAAGGGCGTCATTGCATCGTCTCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTg                                                                                                                                <  6:267179/149‑1 (MQ=255)
                    tCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                                    >  1:242380/1‑130 (MQ=255)
                    tCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCgg                                                                                                                                                    <  2:242380/130‑1 (MQ=255)
                       aCTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTg                                                                                                                                >  2:100375/1‑145 (MQ=255)
                        ctgctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagat                                                                                                                           <  6:160730/149‑1 (MQ=255)
                         tgctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagat                                                                                                                           >  1:352078/1‑148 (MQ=255)
                           ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcag                                                                                                                        <  4:53818/149‑1 (MQ=255)
                           ctgGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagat                                                                                                                           >  5:209599/1‑146 (MQ=255)
                              gAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagag                                                                                                                      <  6:248222/148‑1 (MQ=255)
                               aaGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCATGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                                         >  5:41429/1‑144 (MQ=255)
                                aGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagat                                                                                                                    >  7:65451/1‑148 (MQ=255)
                                 gggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCTGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatca                                                                                                                  <  1:274875/149‑1 (MQ=255)
                                  ggCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 <  6:278809/149‑1 (MQ=255)
                                   gCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 >  5:123679/1‑148 (MQ=255)
                                    cGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 >  6:170369/1‑147 (MQ=255)
                                     gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGGAGATCTAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                                         <  2:338035/138‑1 (MQ=255)
                                     gTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                                         >  1:338035/1‑138 (MQ=255)
                                         tGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAAt                                                                                                           >  1:245/1‑148 (MQ=255)
                                            cATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACt                                                                                                       <  1:169935/149‑1 (MQ=255)
                                                gtcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAAt                                                                                                   >  7:84466/1‑149 (MQ=255)
                                                 tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                                     >  5:387437/1‑146 (MQ=255)
                                                 tcgCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCa                                                                                                     <  6:387437/146‑1 (MQ=255)
                                                   gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 <  1:62723/132‑1 (MQ=255)
                                                   gCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 >  2:62723/1‑132 (MQ=255)
                                                   gCCAATCCACGCTTCGAAGAAGTCACCAAGGAGGTACAGGGCCTTGGCATGGCGGGGGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                                         >  8:248323/1‑124 (MQ=255)
                                                   gCCAATCCACGCTTCGAAGAAGTCACCAAGGACGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatca                                                                                                                         <  7:248323/124‑1 (MQ=255)
                                                      aaTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCTGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGTGAAATATCCGGGCGTTCTTCTTGCA‑‑GGAGCAGATCAGAGATCAGCAGAATCACTCAATGAactc                                                                                             >  2:225200/1‑149 (MQ=255)
                                                       aTCCACGCTTCGAAGAATTCACAAAGGATGTACAGGGCCTTGGCATGGCGCTCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCAGCGCGTTCTTCTTGAA‑‑GGTCCAGAACAGAGATCAGCAGAATcaatcaatatatcg                                                                                             >  6:377985/1‑142 (MQ=255)
                                                       aTCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCaa                                                                                                    <  5:377985/141‑1 (MQ=255)
                                                          cACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCt                                                                                         <  5:170369/149‑1 (MQ=255)
                                                            cGCTTCGAAGAAGTCACCAAGGTTGTACATGGCCTTGGCATGGCTGGCGCGGCCATCGAGGAGATCAAGAAACGCCCGGGAAATATCCGTGCGTTCTTCTTGCA‑‑GGTGCATATCAGAGATCAGCAGAATCACTCAATGATCTCGGCttt                                                                                       <  1:225200/149‑1 (MQ=255)
                                                                 ctaagaagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGTCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTtctc                                                                                    <  5:346085/145‑1 (MQ=255)
                                                                  gtagaagTCACCAAGGATGTACAGGGCCTTTGCATTGGGGGCGCGGCCATCGACCATATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCaga                                                                                                                            <  8:140945/104‑1 (MQ=255)
                                                                  gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTGTTGCA‑‑GGTGCaga                                                                                                                            >  7:140945/1‑106 (MQ=255)
                                                                  gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑TGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                                 <  8:65451/149‑1 (MQ=255)
                                                                  gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                                 >  5:38208/1‑149 (MQ=255)
                                                                  gaagaaGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCgat                                                                                 >  7:127874/1‑149 (MQ=255)
                                                                      aagTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                                             <  6:123679/149‑1 (MQ=255)
                                                                         tCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcg                                                                          >  3:37544/1‑149 (MQ=255)
                                                                          cACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTGCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgt                                                                         >  4:162472/1‑149 (MQ=255)
                                                                            ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 <  4:51389/107‑1 (MQ=255)
                                                                            ccAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCagatcagagatcag                                                                                                                 >  3:51389/1‑107 (MQ=255)
                                                                              aaGGATGTACAGGGCCTTGGCATTGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCACCGGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCAcgtcg                                                                       <  1:305854/149‑1 (MQ=255)
                                                                              aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                                             <  4:83418/141‑1 (MQ=255)
                                                                              aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATc                                                                             >  3:83418/1‑141 (MQ=255)
                                                                              aaGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACgtcgtc                                                                     <  6:41429/149‑1 (MQ=255)
                                                                               aGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTcc                                                                    <  3:195089/149‑1 (MQ=255)
                                                                                  aTGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTc                                                                 <  3:43511/149‑1 (MQ=255)
                                                                                  aTGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTc                                                                 <  2:115866/149‑1 (MQ=255)
                                                                                     tACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGt                                                              <  6:38208/149‑1 (MQ=255)
                                                                                       cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTAc                                                            >  2:149139/1‑149 (MQ=255)
                                                                                       cAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTAc                                                            <  2:352078/149‑1 (MQ=255)
                                                                                          ggCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATAAAGAAACGCCAGGGAAATATCAGGTCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATAAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTc                                                         >  4:225125/1‑149 (MQ=255)
                                                                                           gCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTcc                                                        <  2:245/149‑1 (MQ=255)
                                                                                                ggCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCtggt                                                    <  8:84466/148‑1 (MQ=255)
                                                                                                         ggcgCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCttt                                          <  8:127874/149‑1 (MQ=255)
                                                                                                              ggCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAAc                                     >  5:118423/1‑149 (MQ=255)
                                                                                                                  aTCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTACTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGt                                 >  2:196709/1‑149 (MQ=255)
                                                                                                                   tCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTg                                <  6:209599/149‑1 (MQ=255)
                                                                                                                     gAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTACTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTgg                               <  1:196709/148‑1 (MQ=255)
                                                                                                                           aTCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttt                        >  5:60109/1‑149 (MQ=255)
                                                                                                                            aagaagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACtttt                       >  3:214342/7‑149 (MQ=255)
                                                                                                                               aagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt                      <  2:31779/144‑1 (MQ=255)
                                                                                                                               aagaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTc                     >  3:349011/4‑148 (MQ=255)
                                                                                                                               aagaaCGCCCGGATATTTCCCGGGCGTTCTACTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACttttt                      >  1:31779/4‑147 (MQ=255)
                                                                                                                                 gaaCGCCCGGATATTTCCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt                  >  7:32667/2‑149 (MQ=255)
                                                                                                                                  aaCGCCCTGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGAt                  <  4:37544/148‑1 (MQ=255)
                                                                                                                                                 tCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCTTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGACCACTTCa  >  1:249511/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
CCGGCGCATGGCCTGGCAGATCGACTGCTGGAAGGGCGTCATGGCATCGTCGCCAATCCACGCTTCGAAGAAGTCACCAAGGATGTACAGGGCCTTGGCATGGCGGGCGCGGCCATCGAGCAGATCAAGAAACGCCCGGGAAATATCCGGGCGTTCTTCTTGCA‑‑GGTGCAGATCAGAGATCAGCAGAATCACTCAATGATCTCGGCTTTCTCGATGATCACGTCGTCCTTCGGTACGTCCTGGTGGCCAGCTTTGGAACCGGTGGCCACTTTTTCGATGGCGTCGACCACTTCA  >  NC_002947/3300715‑3301008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑