Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,408,042	T→C	6.0%	N168D (AAC→GAC)	PP_3021 ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,408,042	0	T	C	6.0%	195.6 / 2.3	67	N168D (AAC→GAC)	PP_3021	LysE family transporter
Reads supporting (aligned to +/- strand):  ref base T (29/34);  new base C (2/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

CACCTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCC  >  NC_002947/3407897‑3408189                                                                                                                                                  |                                                                                                                                                           cACCTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGc                                                                                                                                                          <  3:222883/148‑1 (MQ=255)    cTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCCCCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑t                                                                                                                                                   >  4:113545/1‑149 (MQ=255)    cTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑t                                                                                                                                                   >  6:162168/1‑149 (MQ=255)       ggTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑Tg                                                                                                                                                  <  2:138955/147‑1 (MQ=255)           gACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑Cg                                                                                                                                         >  7:163129/1‑149 (MQ=255)            aCATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCAAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑Cgg                                                                                                                                        >  4:172987/1‑149 (MQ=255)             cATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGc                                                                                                                                       <  3:369380/149‑1 (MQ=255)                gcgcACCTTGCAACTAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGAc                                                                                                                                    >  7:328787/1‑149 (MQ=255)                 cgcACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGAc                                                                                                                                    <  8:280357/148‑1 (MQ=255)                      cTTGCAACAAGTCAGCCGCGGGCCAGATGCAGCAACAACGCCCCCGCCTCCATCACCCGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGGTGCCTA‑‑‑TG‑‑GCCA‑CGGCGACCAGCaa                                                                                                                              <  4:52647/149‑1 (MQ=255)                      cTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                              <  4:143978/149‑3 (MQ=255)                       ttGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAg                                                                                                                             >  8:270320/1‑149 (MQ=255)                         gCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGt                                                                                                                            <  3:265716/148‑1 (MQ=255)                           aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCa                                                                                                                                  <  3:2599/140‑1 (MQ=255)                           aacaaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCa                                                                                                                                  >  4:2599/1‑140 (MQ=255)                             caaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCag                                                                                                                       >  1:381827/1‑149 (MQ=255)                              aaGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCaga                                                                                                                      <  8:212652/149‑1 (MQ=255)                                    gCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑a                                                                                                               <  7:119047/149‑1 (MQ=255)                                             cAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGa                                                                                                      >  6:306739/1‑149 (MQ=255)                                               gATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAAc                                                                                                    <  7:221305/149‑1 (MQ=255)                                                   cagcaACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATc                                                                                                 <  3:366414/148‑1 (MQ=255)                                                     gcaACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCa                                                                                               >  1:158110/1‑148 (MQ=255)                                                         caacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCTCTCCAGCCACGCCAGGCTGCGTGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAgcg                                                                                          <  5:299805/149‑1 (MQ=255)                                                         caacGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAgcg                                                                                          <  1:133069/149‑1 (MQ=255)                                                          aacgccaacgccAACATCACCACCATGACCCCCGAGGCCCGCACCAGCCACGCCAAGCAGCGGGCGAAGCGCCGCAGAACCCCCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCACCAACTACCAGAGCAAG‑ACGATGCTGAACAACCATAgcgc                                                                                         >  5:273811/1‑149 (MQ=255)                                                            cGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGcgcg                                                                                        >  6:288849/1‑148 (MQ=255)                                                               ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCTAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAgg                                                                                    <  5:299921/149‑1 (MQ=255)                                                               ccccGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAgg                                                                                    >  2:211511/1‑149 (MQ=255)                                                                  cGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCg                                                                                 >  3:133610/1‑149 (MQ=255)                                                                   gCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGt                                                                                >  1:29018/1‑149 (MQ=255)                                                                    ccAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGtt                                                                               <  8:163129/149‑1 (MQ=255)                                                                         aTCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTc                                                                            >  2:263305/1‑147 (MQ=255)                                                                             ccAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGGTTTCCAGCCg                                                                      >  1:49137/1‑149 (MQ=255)                                                                                gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGTGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                   <  6:273811/149‑1 (MQ=255)                                                                                gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                   <  5:162168/149‑1 (MQ=255)                                                                                gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                   <  5:306739/149‑1 (MQ=255)                                                                                gCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                   <  1:211511/149‑1 (MQ=255)                                                                                 cATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGc                                                                  <  1:62115/149‑1 (MQ=255)                                                                                   tGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCAAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCACG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTg                                                                <  1:248803/149‑1 (MQ=255)                                                                                       ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                   >  7:353242/1‑142 (MQ=255)                                                                                       ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCgggg                                                                   <  8:353242/142‑1 (MQ=255)                                                                                       ccccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGc                                                            >  5:42740/1‑149 (MQ=255)                                                                                         ccGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                          <  2:49137/149‑1 (MQ=255)                                                                                          cGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGCTATCGATTCTGTTGCAA‑GGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGAc                                                              <  1:126909/149‑1 (MQ=255)                                                                                            aGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CCGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGc                                                        >  6:23046/1‑148 (MQ=255)                                                                                               cccGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGAc                                                     >  2:324329/1‑148 (MQ=255)                                                                                                   cTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGc                                                <  2:29018/149‑1 (MQ=255)                                                                                                         ccACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                              <  1:194208/66‑3 (MQ=17)                                                                                                         ccACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCTGCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGcgg                                                                                                                              >  2:194208/1‑64 (MQ=17)                                                                                                          cACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAg                                         >  7:173617/1‑149 (MQ=255)                                                                                                           aCGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAgg                                        >  7:75051/1‑149 (MQ=255)                                                                                                             gCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                          >  1:149884/1‑129 (MQ=255)                                                                                                             gCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTg                                                          <  2:149884/129‑1 (MQ=255)                                                                                                                  gCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCAt                                 <  6:93209/149‑1 (MQ=255)                                                                                                                        ggCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCATG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACa                           <  3:172987/149‑1 (MQ=255)                                                                                                                          cGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAgc                         <  7:270320/149‑1 (MQ=255)                                                                                                                              gcgcCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGGTACGATGCTGAACATCCATAGCGGGTAGGCGGTTTTCCAGCCGGGGATGACGGTGGGGACCATGCTGGCCAGGCTGGCATAGAACAGggcg                      >  1:112115/1‑149 (MQ=255)                                                                                                                              gcgcCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCAAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGt                     >  5:274891/1‑149 (MQ=255)                                                                                                                                gccgcAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTc                   >  5:201797/1‑149 (MQ=255)                                                                                                                                    cAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgg               <  5:23046/149‑1 (MQ=255)                                                                                                                                     aGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTggg              <  4:193433/149‑1 (MQ=255)                                                                                                                                      gTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgggg             <  1:263305/149‑1 (MQ=255)                                                                                                                                      gTACCCACTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTgggg             >  4:123538/1‑149 (MQ=255)                                                                                                                                         cccGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt           <  5:288849/148‑1 (MQ=255)                                                                                                                                         cccGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTg          <  2:381827/149‑1 (MQ=255)                                                                                                                                          ttgCTGGTCGCCG‑‑‑‑TG‑‑GCCATCGGCAACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGtt           >  3:370751/3‑147 (MQ=255)                                                                                                                                                gTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGc   >  4:69637/1‑149 (MQ=255)                                                                                                                                                 ttGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGcc  <  6:201797/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                           CACCTTGGTCGACATGCGCACCTTGCAACAAGTCAGCCCCGGGCCAGATGCAGCAACAACGCCCCCGCCAACATCACCAGCATGACCCCCGAGGCCCGCTCCAGCCACGCCAGGCTGCGGGCGAAGCGCCGCAGTACCCGCTGGTTGCCGA‑‑‑TG‑‑GCCA‑CGGCGACCAGCAAGTCCCAGAGCAGG‑ACGATGCTGAACATCCATAGCGCGTAGGCCGTTTTCCAGCCGGGGCTGACGCTGGCGACCATGCTGGCCAGGCTGGCATAGAACAGCGCGTTCTTGGGGTTGAGGATGCC  >  NC_002947/3407897‑3408189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑