Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,430,686 A→G 6.9% K302E (AAG→GAG)  PP_3045 → ATP‑dependent protease ClpP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,430,6860AG 6.9% 166.4 / 2.3 58K302E (AAG→GAG) PP_3045ATP‑dependent protease ClpP
Reads supporting (aligned to +/- strand):  ref base A (28/26);  new base G (2/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCGAGATCGAC  >  NC_002947/3430539‑3430826
                                                                                                                                                   |                                                                                                                                            
gctccGAATTCAAAAGACTCAGCAGCCTTGGCCTTTAAGATCACCCACGCATGCATCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAATCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCaa                                                                                                                                             <  3:41102/146‑1 (MQ=255)
     gAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCgg                                                                                                                                        <  2:208388/149‑1 (MQ=255)
      aaTGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCgg                                                                                                                                        >  3:112663/1‑148 (MQ=255)
                 cTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACctct                                                                                                                            >  7:202307/1‑149 (MQ=255)
                  tCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTg                                                                                                                           >  8:11250/1‑149 (MQ=255)
                  tCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTg                                                                                                                           >  8:135931/1‑149 (MQ=255)
                  tCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTg                                                                                                                           >  3:28689/1‑149 (MQ=255)
                    agcagcCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCg                                                                                                                         >  6:341628/1‑149 (MQ=255)
                      cagcCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGtt                                                                                                                       <  3:332270/149‑1 (MQ=255)
                       agcCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTg                                                                                                                      <  8:202307/149‑1 (MQ=255)
                        gccttggccttgTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGc                                                                                                                     <  7:161771/149‑1 (MQ=255)
                         ccttggccttgTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCa                                                                                                                    <  4:69932/149‑1 (MQ=255)
                             ggccttgTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGccc                                                                                                                >  6:349083/1‑149 (MQ=255)
                                 ttgttgATCACCCAGGCCCGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCtt                                                                                                            >  5:303493/1‑149 (MQ=255)
                                   gttgATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGc                                                                                                          >  1:181996/1‑149 (MQ=255)
                                        tCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGccc                                                                                                        >  6:38620/1‑146 (MQ=255)
                                         cACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGccc                                                                                                        >  2:142697/1‑145 (MQ=255)
                                            ccAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAATCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCGAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCACGAGATGa                                                                                                 >  3:212350/1‑149 (MQ=255)
                                            ccAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCGAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGa                                                                                                 >  3:207340/1‑149 (MQ=255)
                                            ccAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGa                                                                                                 >  8:26122/1‑149 (MQ=255)
                                                         aaGGCGGTGATCAGCAACCTGGTGGAGCCTATGATTGCATCGACCAAGATTGCGTATGTAGCCACCGTGCAGGCAGCACTGACCCGCTCCGAGTCGGTACTGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGt                                                                                    <  4:212350/149‑1 (MQ=255)
                                                         aaGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCGAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGt                                                                                    <  4:207340/149‑1 (MQ=255)
                                                          aGGCGGGGATAAGCAACCTGCTTGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCCGTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTc                                                                                   <  4:61356/149‑1 (MQ=255)
                                                            gCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTc                                                                                   >  1:75326/1‑147 (MQ=255)
                                                            gCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCa                                                                                 <  7:11250/149‑1 (MQ=255)
                                                                  aTCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAggccgg                                                                           >  7:299051/1‑149 (MQ=255)
                                                                   tCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAggccggc                                                                          >  6:200057/1‑149 (MQ=255)
                                                                      gCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGAGCGCTGAGTTCGTCCAGGCCGGCGTg                                                                       >  2:385324/1‑149 (MQ=255)
                                                                       cAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAggccggc                                                                          >  2:370473/1‑145 (MQ=255)
                                                                       cAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAggccggc                                                                          <  1:370473/145‑1 (MQ=255)
                                                                       cAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAg                                                                                >  5:273462/1‑139 (MQ=255)
                                                                       cAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAg                                                                                <  6:273462/139‑1 (MQ=255)
                                                                       cAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTg                                                                       >  3:193238/1‑148 (MQ=255)
                                                                              gtggAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGacgaact                                                               <  4:28689/149‑7 (MQ=255)
                                                                                 gAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACgg                                                             <  5:349083/148‑1 (MQ=255)
                                                                                  aGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGcc                                                           <  7:135931/149‑1 (MQ=255)
                                                                                              cATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCgg                                               >  8:274656/1‑149 (MQ=255)
                                                                                              cATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCgg                                               <  7:35640/149‑1 (MQ=255)
                                                                                                tCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGgtt                                             <  7:26122/149‑1 (MQ=255)
                                                                                                   aCCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGgttgtt                                          <  6:153614/149‑1 (MQ=255)
                                                                                                   aCCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGgttgtt                                          <  5:200057/149‑1 (MQ=255)
                                                                                                    ccAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTc                                         <  2:75326/149‑1 (MQ=255)
                                                                                                    ccAAGCTTGCTGATGAAGCCACCGTCCACGCAGCACTGACCCGCGCCAAGGCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTc                                         <  8:139079/149‑1 (MQ=255)
                                                                                                              tgatgaAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTgg                               >  8:245110/1‑149 (MQ=255)
                                                                                                                atgaAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTc                             >  1:145917/1‑149 (MQ=255)
                                                                                                                   aaGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGt                          <  4:112663/149‑1 (MQ=255)
                                                                                                                    aGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTg                         <  7:274656/149‑1 (MQ=255)
                                                                                                                     gCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTgg                        <  4:193238/149‑1 (MQ=255)
                                                                                                                       cACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTggcg                      <  5:38620/149‑1 (MQ=255)
                                                                                                                        aCCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTggcgg                     >  4:168870/1‑149 (MQ=255)
                                                                                                                          cGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTggcggc                    >  3:167040/1‑148 (MQ=255)
                                                                                                                               aggcagCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTgg                               >  4:367452/1‑132 (MQ=255)
                                                                                                                               aggcagCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTgg                               <  3:367452/132‑1 (MQ=255)
                                                                                                                                   agcaCTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCg          <  1:142697/149‑1 (MQ=255)
                                                                                                                                        tGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCgaga       >  7:302021/1‑147 (MQ=255)
                                                                                                                                        tGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCgaga       <  8:302021/147‑1 (MQ=255)
                                                                                                                                          ccccTCGACAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCGAGATc     <  8:299051/146‑1 (MQ=255)
                                                                                                                                          aCCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCGAGATCGa   >  7:265456/1‑149 (MQ=255)
                                                                                                                                            ccGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGACCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCGAGATCGac  >  7:138094/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
GCGCCGAATGCAAACGACTCAGCAGCCTTGGCCTTGTTGATCACCCAGGCCTGCAGCAAGGCGGGGATCAGCAACCTGGTGGAGCCGCTGATTGCATCGACCAAGCTTGCTGATGAAGCCACCGTGCAGGCAGCACTGACCCGCGCCAAGTCGGTACGGGACCTCTGCGTTGCAGCCCGCTTGCCCGAGATGACCGCTGAGTTCGTCCAGGCCGGCCTGGATGCAACGGCCGTACGTGCGCGGTTGTTCGACAAATTGGTCGGTGGCGGCAAGGGATTCGAGATCGAC  >  NC_002947/3430539‑3430826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑