Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,446,643	A→G	10.0%	Q346R (CAG→CGG)	gpD →	tail formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,446,643	0	A	G	10.0%	159.4 / 7.7	60	Q346R (CAG→CGG)	gpD	tail formation
Reads supporting (aligned to +/- strand):  ref base A (29/25);  new base G (3/3);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

GATGGCGAGTACCTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATGGGGAAGTTCGC  >  NC_002947/3446498‑3446787                                                                                                                                                  |                                                                                                                                                  gATGGCGAGTACCTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCt                                                                                                                                                <  8:28675/149‑1 (MQ=255)   tGGCGAGTACCTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCTGCTCAACCTTGAGCGGCttg                                                                                                                                              <  7:213699/149‑3 (MQ=255)            ccTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACg                                                                                                                                     >  2:294376/1‑149 (MQ=255)             cTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACgg                                                                                                                                    <  1:186629/149‑1 (MQ=255)             cTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACgg                                                                                                                                    >  3:105089/1‑149 (MQ=255)                 tggACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGt                                                                                                                                <  2:137214/149‑1 (MQ=255)                      ggggTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAACGACCCCAAGCCGCTCAAGCTTGAGAAGCTGTAACCCCCAGTCCGTacacc                                                                                                                           >  8:295267/1‑148 (MQ=255)                        ggTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGc                                                                                                                         >  8:251278/1‑149 (MQ=255)                        ggTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGc                                                                                                                         >  8:200796/1‑149 (MQ=255)                            gAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCAt                                                                                                                      >  1:100041/1‑148 (MQ=255)                              gCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCAc                                                                                                                   >  4:282951/1‑149 (MQ=255)                              gCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCAc                                                                                                                   >  4:19085/1‑149 (MQ=255)                              gCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCAc                                                                                                                   >  7:194595/1‑149 (MQ=255)                                aGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTg                                                                                                                 <  3:38464/149‑1 (MQ=255)                                  gTATTTACCCAGGCCGGCTGGACTACGACCGTCGAGTCCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCCGGCAAGAAAAACAAAGACCCCACGCCGCGCACGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGga                                                                                                               <  8:357450/149‑1 (MQ=255)                                    aTTTACACAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACACCACGGCCGTACACGGCCATCACTGgag                                                                                                              >  2:249108/1‑148 (MQ=255)                                          ccAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCaa                                                                                                       >  3:80784/1‑149 (MQ=255)                                                 ggCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGaa                                                                                                            <  1:93703/137‑1 (MQ=255)                                                 ggCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGaa                                                                                                            >  2:93703/1‑137 (MQ=255)                                                 ggCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTAt                                                                                                <  3:19085/149‑1 (MQ=255)                                                              ccGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGAAGCTGTAACCAAACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCAATATCAGTTCAACAg                                                                                   >  5:236580/1‑149 (MQ=255)                                                                 tCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTg                                                                                >  2:1131/1‑149 (MQ=255)                                                                   gAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCCCGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGc                                                                               >  6:112140/1‑148 (MQ=255)                                                                          aCGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGAtc                                                                       <  1:294376/149‑1 (MQ=255)                                                                             gcggcAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGAtcctc                                                                    <  3:284329/149‑1 (MQ=255)                                                                                  aagaagGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGCCACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑aa                                                              >  1:333273/1‑149 (MQ=255)                                                                                   agaagGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AAc                                                             <  4:105089/149‑1 (MQ=255)                                                                                    gaagGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACg                                                            >  6:219340/1‑149 (MQ=255)                                                                                    gaagGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACg                                                            >  6:224426/1‑149 (MQ=255)                                                                                      agGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACgcc                                                          >  1:298634/1‑149 (MQ=255)                                                                                         gCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCAACACTGGAGAAACCAATGGCTATCTCAGTTCACCAGCTGCAACACATCCTCCCC‑AACgccggc                                                       >  6:306483/1‑149 (MQ=255)                                                                                          caaggccaaggcCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACgccggcc                                                      <  2:100041/149‑1 (MQ=255)                                                                                            aggccaaggccTCGGGCAAGAAAAAGAAAGAGACCAAGCCTCTCAAGGTTGAGCAGCTGTAACCCCACGGGCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGc                                                    <  8:340209/149‑1 (MQ=255)                                                                                               ccaaggccTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGt                                                                                          >  4:52708/1‑109 (MQ=255)                                                                                               ccaaggccTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGt                                                                                          <  3:52708/109‑1 (MQ=255)                                                                                                      cTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCg                                          >  6:33466/1‑149 (MQ=255)                                                                                                      cTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGACCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCg                                          >  2:304212/1‑149 (MQ=255)                                                                                                         gggCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCTCAAAGCCGGCGttt                                       <  5:112140/149‑1 (MQ=255)                                                                                                          ggCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTa                                      <  3:282951/149‑1 (MQ=255)                                                                                                                 aaaagaaaGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTccc                               >  1:21080/1‑149 (MQ=255)                                                                                                                  aacgaaagaCACCAAGCCGCTCAAGGATGAGCAGCTGTAACCCCACCGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCg                              <  8:356075/146‑1 (MQ=255)                                                                                                                   aagaaagaCACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCCAAACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCg                              <  1:249108/149‑1 (MQ=255)                                                                                                                    agaaagaCACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGa                            <  1:1131/149‑1 (MQ=255)                                                                                                                        agaCACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCa                        <  8:194595/149‑1 (MQ=255)                                                                                                                            ttacaGCTGCTCAACCTTGAGCGGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCgg                                            <  6:178450/121‑1 (MQ=255)                                                                                                                            ttacaGCTGCTCAACCTTGAGCGGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCgg                                            >  5:178450/5‑125 (MQ=255)                                                                                                                              cAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGcta                  >  1:228836/1‑149 (MQ=255)                                                                                                                              cAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGcta                  >  1:231678/1‑149 (MQ=255)                                                                                                                              cAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGct                   >  5:225925/1‑148 (MQ=255)                                                                                                                              cAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGct                   >  5:179803/1‑148 (MQ=255)                                                                                                                                aGCTGCTCAACCTTGAGCGGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTccc                               <  1:354775/134‑1 (MQ=255)                                                                                                                                aGCTGCTCAACCTTGAGCGGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTccc                               >  2:354775/1‑134 (MQ=255)                                                                                                                                  ctgCTCAACCTTGAGCGGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTAt              >  3:166118/3‑149 (MQ=255)                                                                                                                                   cGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATg             <  2:298634/149‑1 (MQ=255)                                                                                                                                    gCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATgg            <  6:179803/149‑1 (MQ=255)                                                                                                                                     cTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACATGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATggg           <  6:300690/149‑1 (MQ=255)                                                                                                                                     cTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATggg           >  1:157653/1‑149 (MQ=255)                                                                                                                                        aaGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCGACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATGGGGaa        <  7:200796/149‑1 (MQ=255)                                                                                                                                        aaGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATGGGGaa        <  7:251278/149‑1 (MQ=255)                                                                                                                                              gAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGACACCAATGGCTATCTCAGTTCAACACCTGCAACAGATCCTCCCC‑AACGCCGGCCGGAAAGCCGGCGTTTATGTTCCCGGACTCCACGCTACTATGGGGAAGTTCg   <  2:333442/148‑1 (MQ=255)                                                                                                                                               agcagcTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATGGGGAAGTTCGc  <  2:333273/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GATGGCGAGTACCTGGTGGACGGGGTCGAGCAGGTATTTACCCAGTCCGGCTGGACTACGACCGTCGAGTGCAACGGCGGCAAGAAGGGCAAGGCCAAGGCCTCGGGCAAGAAAAAGAAAGACACCAAGCCGCTCAAGGTTGAGCAGCTGTAACCCCACGGCCGTACACGGCCATCACTGGAGAAACCAATGGCTATCTCAGTTCAACAGCTGCAACAGATCCTCCCC‑AACGCCGGCCGCAAAGCCGGCGTTTATGTTCCCGGACTCAACGCTACTATGGGGAAGTTCGC  >  NC_002947/3446498‑3446787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑