Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,447,284 C→T 7.0% A17V (GCC→GTC)  PP_5556 → bacteriophage lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,447,2840CT 7.0% 164.9 / 2.0 57A17V (GCC→GTC) PP_5556bacteriophage lysis protein
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base T (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

CCTGGCGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGC  >  NC_002947/3447148‑3447431
                                                                                                                                        |                                                                                                                                                    
ccTGGCGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggcgg                                                                                                                                          >  1:84067/1‑149 (MQ=255)
    gCGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGcc                                                                                                                                      >  7:204103/1‑149 (MQ=255)
     cGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGccc                                                                                                                                     <  7:103225/149‑1 (MQ=255)
       aTCACCAAAGTTATCAATGGGGGTCGCAACGGTCTGGAGGATCGCAAGGCCCTCTACACGCGAGCGCTCGAGGTGCTGCCGGGCGCGCTTGGGCCACCCGCCTGGGCGCCGTGGGGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCgg                                                                                                                                   <  1:120333/149‑1 (MQ=255)
       aTCACCAAACTTATCAATGGTGGTAGCAAGGGTCTGGCGGATCGCAAGGCCCTCTACAAGCGAGCGCTGGAGGTGCTGCTGTGAGCGCTTGGGCCACCCGCCGGGTCGCGGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCgg                                                                                                                                   <  4:336590/149‑1 (MQ=255)
          aCCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGAAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCa                                                                                                                                <  8:644/149‑1 (MQ=255)
            cAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGc                                                                                                                              >  5:94940/1‑149 (MQ=255)
             aaaCGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGcc                                                                                                                             >  7:289214/1‑149 (MQ=255)
              aaCGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggc                                                                                                                                            <  1:351387/133‑1 (MQ=255)
              aaCGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggc                                                                                                                                            >  2:351387/1‑133 (MQ=255)
                      attgggggTAGCAACGGTCTGCAAGATCGCAAGGCCCTCTACAAGCGCGGGCTCGAGGGGCTGCTGTGAGCGGTTGGCCCACCCGCCTGGTCGCCGTGGCGCGGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTgg                                                                                                                    <  2:241227/147‑1 (MQ=255)
                      aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTgg                                                                                                                    >  1:372596/1‑149 (MQ=255)
                        tggtggTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTgg                                                                                                                    >  7:21083/1‑147 (MQ=255)
                        tgggggTAGCAATGGTCTGGCGCATCGCAAGGCCCTCTACAAGCGCGCGCTCGTGGGGCTGCAGGGCGCGCTTGGGCCACCCGCCTGGGCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCa                                                                                                                  <  7:223568/149‑1 (MQ=255)
                                         tGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggcggc                                                                                                                                         <  3:119818/109‑1 (MQ=255)
                                         tGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTggcggc                                                                                                                                         >  4:119818/1‑109 (MQ=255)
                                           gaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGgcg                                                                                              >  3:127084/1‑149 (MQ=255)
                                           gaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGgcg                                                                                              >  3:255384/1‑149 (MQ=255)
                                            cggaCCGCATGCCCCTCTACACGCGCGCGCTCGCGGTGCTGCTGTCAGCGCTTGGGCCACCCGCGTGGGCGCCGGGGCGCTGCTGCGGGCCGCCTGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGgcgc                                                                                             <  4:174838/148‑1 (MQ=255)
                                            aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGTCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGgcg                                                                                              >  8:45227/1‑148 (MQ=255)
                                            aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGTCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGgcg                                                                                              >  6:345424/1‑148 (MQ=255)
                                             ggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGgcg                                                                                              >  7:36060/1‑147 (MQ=255)
                                              gaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAg                                                                                           >  8:204828/1‑149 (MQ=255)
                                                tCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGTCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCt                                                                                         <  5:345424/149‑1 (MQ=255)
                                                tCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGTCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCt                                                                                         <  7:45227/149‑1 (MQ=255)
                                                   cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGc                                                                                      <  4:255384/149‑1 (MQ=255)
                                                   cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCA‑ACGCCGATAAGGCGCCGCTCGc                                                                                      >  2:337548/1‑149 (MQ=255)
                                                         ccTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCa                                                                                <  2:5098/149‑1 (MQ=255)
                                                          ctctACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAg                                                                               >  2:282904/1‑149 (MQ=255)
                                                               cAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGa                                                                          <  8:204103/149‑1 (MQ=255)
                                                                aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGAt                                                                         <  6:94940/149‑1 (MQ=255)
                                                                      gcgcTCTAGGGGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTc                                                                   <  5:279936/149‑1 (MQ=255)
                                                                      gcgcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCAGGGTGTGGCACGCCA‑ACGCCTATAAGGCGCCGCTCGCTGATCAGGCCGATGACGTc                                                                   >  6:290746/1‑149 (MQ=255)
                                                                         gtCGTGGTGCTGAAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGc                                                                <  2:84067/148‑1 (MQ=255)
                                                                            gAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAg                                                             >  3:238603/1‑149 (MQ=255)
                                                                              ggggcggcAGGGAGCGGTTGGGCCCCCCGCCTGGTCGCCGTGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCa                                                           <  1:337548/143‑1 (MQ=255)
                                                                                  ctgcAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg                                                       >  5:75878/1‑149 (MQ=255)
                                                                                   tgcAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTgg                                                      >  8:9166/1‑149 (MQ=255)
                                                                                       gTGAGGGCTTGGGCCACCCGCCTGGGCGCCGTGGCGGTGCTGCGGGCCGCCAGCGCTGGGTGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGga                                                  <  1:77538/149‑1 (MQ=255)
                                                                                          cgcgcTTGGGCCACCCGCCTGGGCGCCGTGGCGCTGGGGCGGCCCGCCAGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGaa                                               <  8:289214/148‑1 (MQ=255)
                                                                                          aGCGGTTGGGCCACCCGCGTGGTCGGGGTGGCGCTGATGCTGGCCGCCAGCGGGGGGGGCGCCCGGGCAGCCTGTGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGaa                                               <  5:189663/149‑1 (MQ=255)
                                                                                            cgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAgg                                             <  8:21083/149‑1 (MQ=255)
                                                                                            cgcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAg                                              >  1:28362/1‑148 (MQ=255)
                                                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAATGCGCAGGTCGCTCATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGCAgg                                             >  3:226609/1‑148 (MQ=255)
                                                                                                   gCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                      <  4:127084/149‑1 (MQ=255)
                                                                                                        ccGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGaa                                               <  6:117922/135‑1 (MQ=255)
                                                                                                        ccGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGaa                                               >  5:117922/1‑135 (MQ=255)
                                                                                                        ccGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACg                                 >  3:53650/1‑149 (MQ=255)
                                                                                                          gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGt                               >  8:267830/1‑149 (MQ=255)
                                                                                                               gTCGCCGGGGGGCTGCTGCGGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg                            <  5:185093/147‑1 (MQ=255)
                                                                                                                  gCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCg                       <  6:75878/149‑1 (MQ=255)
                                                                                                                   ccGTGGCGCGGCTGCTGGCGGCCTGGGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCg                       <  4:226609/148‑1 (MQ=255)
                                                                                                                    cGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCggg                     <  6:199876/149‑1 (MQ=255)
                                                                                                                      tGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCGATAAGGCGCCGCTCGCGGATCAGGCCGCTGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACgg                                >  7:333053/1‑136 (MQ=255)
                                                                                                                        gggctggtgctGGCCGCCTGGGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                 <  5:290746/147‑1 (MQ=255)
                                                                                                                                gctgGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcag         >  5:254053/1‑149 (MQ=255)
                                                                                                                                  tgGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAGACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcag         >  1:90306/1‑148 (MQ=255)
                                                                                                                                     ccgccAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑CCGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGACAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcag      >  8:113877/1‑147 (MQ=255)
                                                                                                                                       gccAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCagcagcagc  >  2:110261/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                                    
CCTGGCGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCA‑ACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGC  >  NC_002947/3447148‑3447431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑