Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,453,480	A→G	7.3%	intergenic (‑34/+79)	PP_3069 ← / ← ppiC‑I	outer membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,453,480	0	A	G	7.3%	187.6 / 4.6	69	intergenic (‑34/+79)	PP_3069/ppiC‑I	outer membrane autotransporter/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base A (35/28);  new base G (2/3);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

CCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACCACA  >  NC_002947/3453335‑3453625                                                                                                                                                      |                                                                                                                                                  ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTG‑‑‑‑CGCAg                                                                                                                                                <  6:71186/149‑1 (MQ=255) ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTG‑‑‑‑CGCAg                                                                                                                                                <  7:115760/149‑1 (MQ=255) ccgggccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGAGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAg                                                                                                                                                >  1:84714/1‑149 (MQ=255)     gccgggTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCAACAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCCCACCACGCGTGGTG‑‑‑‑CACAGCGt                                                                                                                                             >  6:155846/1‑148 (MQ=255)          gTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCg                                                                                                                                        <  2:74524/148‑1 (MQ=255)          gTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCg                                                                                                                                        >  1:74524/1‑148 (MQ=255)           tGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCggg                                                                                                                                      >  3:353246/1‑149 (MQ=255)              gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGtt                                                                                                                                   >  6:377814/1‑149 (MQ=255)              gaAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGtt                                                                                                                                   >  4:142707/1‑149 (MQ=255)              gaAGGTACAAGCCGCGTGGGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTCACCGGGGtt                                                                                                                                   <  5:344561/149‑1 (MQ=255)               aaGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCGATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTg                                                                                                                                  <  5:155846/149‑1 (MQ=255)                  gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGta                                                                                                                                >  2:315781/1‑148 (MQ=255)                  gTACAAGCCGCGTGTGCAGCGGGCGAGGCCATACCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGta                                                                                                                                >  1:242592/1‑148 (MQ=255)                    aCAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGta                                                                                                                                >  5:100271/1‑146 (MQ=255)                     cAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATaga                                                                                                                            <  6:338959/149‑1 (MQ=255)                      aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATagag                                                                                                                           >  7:156005/1‑149 (MQ=255)                      aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATagag                                                                                                                           <  5:377814/149‑1 (MQ=255)                      aaGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATagag                                                                                                                           >  1:341881/1‑149 (MQ=255)                       aGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAgagg                                                                                                                          <  4:343355/149‑1 (MQ=255)                        gCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATagag                                                                                                                           >  2:211468/1‑147 (MQ=255)                           gcgTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTG‑‑‑‑CGCAgg                                                                                                                                               >  7:166397/1‑124 (MQ=255)                           gcgTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGTGCACCACGCGTGGTG‑‑‑‑CGCAgg                                                                                                                                               <  8:166397/124‑1 (MQ=255)                           gcgTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTg                                                                                                                      >  4:313944/1‑149 (MQ=255)                            cgTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGc                                                                                                                     <  8:61137/149‑1 (MQ=255)                                  cAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCAc                                                                                                                >  2:364469/1‑148 (MQ=255)                                     cGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGAc                                                                                                             >  4:250986/1‑148 (MQ=255)                                               cATGCCCAGCAGCGGGAGCGAACCCAGGGTGAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACGTGCGCAGCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCa                                                                                                  <  6:78437/149‑1 (MQ=255)                                                aTGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCCCGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATaga                                                                                                                            >  2:46744/1‑122 (MQ=255)                                                    ccAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAAt                                                                                             <  7:319270/149‑1 (MQ=255)                                                      agcagcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGt                                                                                           <  8:78353/149‑1 (MQ=255)                                                        cagcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGAGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCa                                                                                         <  2:84714/149‑1 (MQ=255)                                                         agcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTGC‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCa                                                                                         <  1:305635/149‑1 (MQ=255)                                                         agcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAg                                                                                        <  3:313944/149‑1 (MQ=255)                                                          gcGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAgg                                                                                       <  3:269974/149‑1 (MQ=255)                                                           cGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGt                                                                                      >  3:67864/1‑149 (MQ=255)                                                           cGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGt                                                                                      >  1:174190/1‑149 (MQ=255)                                                            gggAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTg                                                                                     >  8:101775/1‑149 (MQ=255)                                                              gAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTgg                                                                                    >  2:241702/1‑148 (MQ=255)                                                                      ccAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGc                                                                            >  5:229954/1‑148 (MQ=255)                                                                       cAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTa                                                                          <  6:229954/149‑1 (MQ=255)                                                                        aGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTACCCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTAACGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAg                                                                         >  8:46046/1‑149 (MQ=255)                                                                          tgTCATCATCACGCGTAGACTCATCACTAAAACCTCAATTCAACAGTTAAACCTGCGCCACACCCGGGGTGGCTACCCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCt                                                                           <  1:315781/148‑1 (MQ=255)                                                                              aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGtt                                                                   <  3:142707/149‑1 (MQ=255)                                                                              aGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGt                                                                    <  1:211468/148‑1 (MQ=255)                                                                               gCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCTCCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTa                                                                  <  4:353246/149‑1 (MQ=255)                                                                               gCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGt                                                                    >  1:225555/1‑147 (MQ=255)                                                                                cATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAg                                                                 >  5:325054/1‑149 (MQ=255)                                                                                 aTCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGt                                                                >  4:213476/1‑149 (MQ=255)                                                                                 aTCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTGGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGt                                                                >  4:336105/1‑149 (MQ=255)                                                                                      gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCgg                                                           <  7:223857/149‑1 (MQ=255)                                                                                      gCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCgg                                                           <  3:213476/149‑1 (MQ=255)                                                                                       cGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCTAAGGGCTAGCGCGTTAGTCGCGGt                                                          <  3:336105/149‑1 (MQ=255)                                                                                         tAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCgg                                                           >  2:351628/1‑146 (MQ=255)                                                                                              gCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGt                                                   <  2:341881/149‑1 (MQ=255)                                                                                                          ccTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtg                                       <  6:325054/149‑1 (MQ=255)                                                                                                           cTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAggtgg                                      >  1:64595/1‑149 (MQ=255)                                                                                                              cATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAg                                   >  6:36774/1‑149 (MQ=255)                                                                                                                   aCCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCGAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGaa                              >  6:385149/1‑149 (MQ=255)                                                                                                                   aCCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGaa                              >  5:359382/1‑149 (MQ=255)                                                                                                                     cAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAAtt                            <  6:100271/149‑1 (MQ=255)                                                                                                                         gAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGct                        >  2:58110/1‑149 (MQ=255)                                                                                                                         gAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGct                        <  1:241702/149‑1 (MQ=255)                                                                                                                          aaaCCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGctc                       <  3:250986/149‑1 (MQ=255)                                                                                                                              cTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGa                   <  7:46046/149‑1 (MQ=255)                                                                                                                               tgtgcACCACGCGTGGTG‑‑‑‑CGCAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGAt                  >  8:161011/4‑149 (MQ=255)                                                                                                                               tGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGAt                  >  8:165694/1‑149 (MQ=255)                                                                                                                                             ggtg‑‑‑‑CACAGGGTTTCAGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGAAATAATGTCAGGTGGCAAAGGGCTAGCGCGTTTGt                                                                >  6:114102/1‑89 (MQ=255)                                                                                                                                             ggtg‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCGAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACca    <  5:385149/149‑1 (MQ=255)                                                                                                                                               tg‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACCACTGGCAAAAATGTCAGCTGGCAAAGGGCTAGCTCGTTAGTCTCGGTAGAACGTTTGCAGCAGGTGGTCGACGAAGTTGGTCTTGATCGGCCAGTGTACcaca  >  6:222068/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CCGGGCCGGGTGAGAAGGTACAAGCCGCGTGTGCAGCGGGCGAGGCCATGCCCAGCAGCGGGAGCGAACCCAGGGTCAGCATCAGGCGTAGACGCATCAGGAAAACCTCCATTCACCAGGGAAACCTGCGCACCACGCGTGGTG‑‑‑‑CACAGGGTTACCGGGGTTGTATAGAGGAGTGCGTCACGACCACAACTGGCAAAAATGTCAGGTGGCAAAGGGCTAGCGCGTTAGTCGCGGTAGAACGTTTGCACCAGGTGGTAGCCGAATTTGCTCTTGATCGGCCCGTGTACCACA  >  NC_002947/3453335‑3453625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑