Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,463,544 G→C 8.0% L272V (CTG→GTG)  PP_3078 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,463,5440GC 8.0% 191.0 / 4.7 61L272V (CTG→GTG) PP_3078ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (27/29);  new base C (2/3);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGC  >  NC_002947/3463396‑3463689
                                                                                                                                                     |                                                                                                                                                 
ccGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAg                                                                                                                                                   <  3:107770/149‑1 (MQ=255)
ccGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAg                                                                                                                                                   <  5:96413/149‑1 (MQ=255)
   ttGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGt                                                                                                                                                 >  6:298841/1‑148 (MQ=255)
      tAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCAc                                                                                                                                              <  8:178333/148‑1 (MQ=255)
        gctcAAGGTGTAGGCCGTGGCGGAGAACTCGCGGGTGTAGTCGTCGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCACGTGAcct                                                                                                                                            <  6:373798/145‑3 (MQ=255)
          ccAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACAGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATCCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TCGCGGGAAGGTCACGTGcc                                                                                                                                         >  1:257678/1‑149 (MQ=255)
           cAAGGTGTAGGCCGTGGCTGAGAAGTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGccc                                                                                                                                        <  4:79269/149‑1 (MQ=255)
           cAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCATGGTGGGCACGTGACCTGcc                                                                                                                                         >  6:37614/1‑149 (MQ=255)
               gtgtAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCccacca                                                                                                                                    <  7:57587/149‑1 (MQ=255)
                      ccGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTTGGCGGGCAGGTCACGTGCCCACCATGCCgg                                                                                                                              <  1:34780/149‑1 (MQ=255)
                       cGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCATGGTGGGCACGTGACCTGCccgccagccgtgg                                                                                                                             <  5:37614/149‑8 (MQ=38)
                          ggCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTcgc                                                                                                                         >  5:188947/1‑149 (MQ=255)
                             ggAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGtt                                                                                                                      <  6:273798/149‑1 (MQ=255)
                             ggAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGtt                                                                                                                      <  5:298841/149‑1 (MQ=255)
                                 aaCTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTGCGGTGTCGCCGAAGTACTCGATGCGGAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGaa                                                                                                                  <  4:47926/149‑1 (MQ=255)
                                  aCTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAg                                                                                                                 <  2:75227/149‑1 (MQ=255)
                                  aCTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAg                                                                                                                 <  6:303374/149‑1 (MQ=255)
                                  aCTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGACACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAg                                                                                                                 <  5:88783/149‑1 (MQ=255)
                                    tCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGt                                                                                                               >  7:197441/1‑149 (MQ=255)
                                       ctgTTTTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGAt                                                                                                            <  2:257678/147‑1 (MQ=255)
                                                ttgTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGcg                                                                                                   >  4:357725/3‑148 (MQ=255)
                                                  gTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCg                                                                                                  <  5:347076/148‑1 (MQ=255)
                                                   tAGCCACCGCCTTTTAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGAtgctgc                                                                                                       <  4:352429/142‑1 (MQ=255)
                                                   tAGCCACCGCCTTTAAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGAtgctgc                                                                                                       >  3:352429/1‑142 (MQ=255)
                                                        aCCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGt                                                                                                                                                 >  8:149702/1‑95 (MQ=255)
                                                        aCCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGt                                                                                                                                                 <  7:149702/95‑1 (MQ=255)
                                                        aCCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGa                                                                                           >  4:117081/1‑149 (MQ=255)
                                                         ccGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGAt                                                                                          <  8:197441/149‑1 (MQ=255)
                                                             cTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGGCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATgcgc                                                                                      >  7:138061/1‑149 (MQ=255)
                                                             cTTTGAGGACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAACTTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATgcgc                                                                                      <  4:46758/149‑1 (MQ=255)
                                                                  aGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGGTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAAt                                                                                 >  8:111828/1‑149 (MQ=255)
                                                                      ccTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCgg                                                                             >  4:160356/1‑149 (MQ=255)
                                                                       cTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTc                                                                                             <  2:204680/132‑1 (MQ=255)
                                                                       cTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTc                                                                                             >  1:204680/1‑132 (MQ=255)
                                                                       cTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCg                                                                                            >  7:243269/1‑133 (MQ=255)
                                                                          gcgcgcCACTTCGGAGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATg                                                                         <  8:138061/149‑1 (MQ=255)
                                                                               ccACTTCGGTGTCGCCGAAGGACTCGATGCGCAGTGTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGGCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCg                                                                    >  2:285800/1‑149 (MQ=255)
                                                                                cACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGc                                                                   <  6:188947/149‑1 (MQ=255)
                                                                                  cTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCTCGTGCCCACGATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGccc                                                                                    <  4:10504/130‑1 (MQ=255)
                                                                                  cTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGccc                                                                                    >  3:10504/1‑130 (MQ=255)
                                                                                        tgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACt                                                           >  4:256325/1‑149 (MQ=255)
                                                                                        tgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACt                                                           >  8:169298/1‑149 (MQ=255)
                                                                                         gtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTg                                                          <  3:160356/149‑1 (MQ=255)
                                                                                            gCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCt                                                       >  2:367827/1‑149 (MQ=255)
                                                                                             ccGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCtt                                                      >  8:254761/1‑149 (MQ=255)
                                                                                              cGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGGAAAATGGCTTg                                                     >  1:134392/1‑149 (MQ=255)
                                                                                               gAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGt                                                    <  3:117081/149‑1 (MQ=255)
                                                                                               gAAGTACTCGATGCGCAGTTTGGCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGt                                                    <  4:130037/149‑1 (MQ=255)
                                                                                                gcatACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGTC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTc                                                   <  1:228618/146‑1 (MQ=255)
                                                                                                           gcgcAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCAc                                        >  4:89801/1‑149 (MQ=255)
                                                                                                             gcAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCg                                      >  7:101998/1‑149 (MQ=255)
                                                                                                               aGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGtt                                    <  7:254761/149‑1 (MQ=255)
                                                                                                                 tttGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTgg                                  <  8:101998/149‑1 (MQ=255)
                                                                                                                     tCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCaaa                              <  3:89801/149‑1 (MQ=255)
                                                                                                                       gAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGt                            >  7:363894/1‑149 (MQ=255)
                                                                                                                       gAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGt                            >  5:217385/1‑149 (MQ=255)
                                                                                                                                      aCGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGt             >  1:390179/1‑149 (MQ=255)
                                                                                                                                             ggtgggCACGTGACCTGCCCGCCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCAc                                        >  2:275884/4‑116 (MQ=255)
                                                                                                                                             ggtgggCACGTGACCTGCCCGCCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCAc                                        <  1:275884/113‑1 (MQ=255)
                                                                                                                                              gCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTc      >  6:167349/1‑149 (MQ=255)
                                                                                                                                                gggCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCgg    <  7:169298/149‑1 (MQ=255)
                                                                                                                                                gggCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCgg    >  1:158499/1‑149 (MQ=255)
                                                                                                                                                  gCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCTGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGc  <  7:323036/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCGTTGTAGCCCAAGGTGTAGGCCGTGGCGGAGAACTCGCGGTTGTAGTCGTAGCCACCGCCTTTGAGTACCTGGCGCGCCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGC‑TGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGATGCGCCCAATGCGGTATGGCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTGGCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGC  >  NC_002947/3463396‑3463689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑