Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,470,866 T→G 8.5% I556L (ATC→CTC) ‡ PP_3084 ← ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,470,8660TG 8.5% 175.6 / 5.0 57I556L (ATC→CTC) ‡PP_3084ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (29/23);  new base G (3/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCCTGGGCCAGGCCCTCGATGCGGTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAGGTATCGGTCGGGCGCGG  >  NC_002947/3470719‑3471013
                                                                                                                                                    |                                                                                                                                                   
gCCCCTGGGCCAGGCCCTCGATGCGGGTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATc                                                                                                                                                    <  8:77318/149‑1 (MQ=255)
  cccTGGGCCAGGCCCTCGATGCGGTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGa                                                                                                                                                  <  7:214278/149‑1 (MQ=255)
    cTGGGCCAGGCCCTCGATGCGGTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGAGCGACt                                                                                                                                                >  1:194033/1‑149 (MQ=255)
     tGGGCCAGGCCCTCGATGCGGTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTg                                                                                                                                               >  8:96739/1‑149 (MQ=255)
         ccAGGCCCTCGATGCGGTTGTCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGATCAGC‑GTCGACAGCTGGGCGACTGccac                                                                                                                                           <  6:261769/149‑3 (MQ=38)
                        ggTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTc                                                                                                                            <  4:49102/149‑1 (MQ=255)
                         gTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTc                                                                                                                            >  1:133155/1‑148 (MQ=255)
                          ttGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCaa                                                                                                                          <  2:211147/149‑1 (MQ=255)
                            ggCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAAc                                                                                                                         <  6:58006/148‑1 (MQ=255)
                             gCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACa                                                                                                                        <  3:187306/148‑1 (MQ=255)
                                   aaCGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGATCAGC‑GTCGACAGCTGGGCGACTGc                                                                                                                                              >  7:190927/1‑120 (MQ=38)
                                   aaCGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGATCAGC‑GTCGACAGCTGGGCGACTGc                                                                                                                                              <  8:190927/120‑1 (MQ=38)
                                    aCGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCgg                                                                                                                >  1:378307/1‑149 (MQ=255)
                                      gACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGcc                                                                                                                                             <  4:350411/118‑1 (MQ=255)
                                      gACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGcc                                                                                                                                             >  3:350411/1‑118 (MQ=255)
                                           aTGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCca                                                                                                         <  7:96739/149‑1 (MQ=255)
                                               tCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCcagcag                                                                                                     >  4:205176/1‑149 (MQ=255)
                                                cccAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCagcagc                                                                                                    <  6:48933/149‑1 (MQ=255)
                                                         tGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGgtg                                                                                           >  1:176691/1‑149 (MQ=255)
                                                           aTCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGAGCGTc                                                                                         >  2:145073/1‑149 (MQ=255)
                                                               ccAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGAGTCGGa                                                                                      >  4:40017/1‑148 (MQ=255)
                                                                    ccATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAAcgc                                                                                <  2:133155/149‑1 (MQ=255)
                                                                      aTACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGa                                                                              <  2:176691/149‑1 (MQ=255)
                                                                          acCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGct                                                                          >  8:255883/1‑149 (MQ=255)
                                                                              gggTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCttg                                                                      >  5:141120/1‑149 (MQ=255)
                                                                                   gtgATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCg                                                                                        <  6:94178/126‑1 (MQ=255)
                                                                                     gATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCg                                                                 <  7:81442/147‑1 (MQ=255)
                                                                                     gATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCg                                                                 >  8:81442/1‑147 (MQ=255)
                                                                                     gATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGt                                                               >  7:21509/1‑149 (MQ=255)
                                                                                     gATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGt                                                               >  4:247435/1‑149 (MQ=255)
                                                                                          aCTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTg                                                          >  8:338303/1‑149 (MQ=255)
                                                                                           cTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGc                                                         <  3:247435/149‑1 (MQ=255)
                                                                                                 gTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGCCGCTCTTGTTGCGGTTCTTGCcgacga                                                   >  1:141706/1‑149 (MQ=255)
                                                                                                  tAAGGCGTGTTGCGCACACGTGTGGCAGTCGCCCAGCTGTCGAC‑GCGGATCGACTGCCGGGGCACTCCCAATGTCAACAGAACGCGGTCATCAAGCAGCCGCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGAt                                                  <  8:355027/149‑1 (MQ=255)
                                                                                                        gtgtTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCgg                                            >  1:335827/1‑149 (MQ=255)
                                                                                                             gcgcACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAAcgc                                                                                >  7:162028/1‑108 (MQ=255)
                                                                                                             gcgcACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAAcgc                                                                                <  8:162028/108‑1 (MQ=255)
                                                                                                             gcgcACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGt                                       >  1:55129/1‑149 (MQ=255)
                                                                                                              cgcACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTg                                      >  5:60434/1‑149 (MQ=255)
                                                                                                              cgcACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGAACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTg                                      >  1:289964/1‑149 (MQ=255)
                                                                                                               gcACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTg                                      >  8:68303/1‑148 (MQ=255)
                                                                                                                 acacCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCa                                    >  7:305162/1‑148 (MQ=255)
                                                                                                                     cTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGCCTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCg                               >  7:23709/1‑149 (MQ=255)
                                                                                                                     cTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCg                               >  7:246987/1‑149 (MQ=255)
                                                                                                                       gtgGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCGAGCAGCCCGAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTATTGCCGACGATGCGCCGGTCGTGCAAATCGCt                             >  6:89035/1‑149 (MQ=255)
                                                                                                                        tgGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCt                             <  3:205176/148‑1 (MQ=255)
                                                                                                                        tgGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGAGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCt                             <  3:40017/148‑1 (MQ=255)
                                                                                                                          gCAGTCGATCAGC‑GTCGACAGCTGGGCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGcc                          >  4:336086/1‑149 (MQ=255)
                                                                                                                             gTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGa                        >  5:367708/1‑148 (MQ=255)
                                                                                                                              tCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGaaa                      <  7:338303/149‑1 (MQ=255)
                                                                                                                               cGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAAt                     <  8:246987/149‑1 (MQ=255)
                                                                                                                                 cccAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAg                   >  5:256534/1‑149 (MQ=255)
                                                                                                                                  ccAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAgg                  >  4:177972/1‑149 (MQ=255)
                                                                                                                                    aGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCTCGCGGTCATCCAGCAGCCCCATGGTGTCGGACAACGCGACGCTCTTGTTGCTGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAGGTa                <  5:44448/149‑1 (MQ=255)
                                                                                                                                    aGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAGGTa                >  3:380693/1‑149 (MQ=255)
                                                                                                                                    aGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAGGTa                <  2:7364/149‑1 (MQ=255)
                                                                                                                                                   aTCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAGGTATCGGTCGGGCGCgg  <  2:335827/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCCTGGGCCAGGCCCTCGATGCGGTTGGCATAGAACGACACATGCTCCCAGGGTTTGATCACCAGGCCATACACCGGGGTTGTGATCGACTCATCGTAAGGCGTGTTGCGCACACCTGTGGCAGTCGCCCAGCTGTCGAC‑GCTGATCGACTGCCGGCGCACGCCCACGGTCAACAGCACGCGGTCATCCAGCAGCCCCAGGGTGTCGGACAACGCGACGCTCTTGTTGCGGTTCTTGCCGACGATGCGCGGGTCGTGCAAATCGCTGCCGAAATAGGTATCGGTCGGGCGCGG  >  NC_002947/3470719‑3471013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑