Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,490,552	A→T	5.3%	L280* (TTA→TAA)	clpV ←	protein ClpV1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,490,552	0	A	T	5.3%	229.4 / 2.5	75	L280* (TTA→TAA)	clpV	protein ClpV1
Reads supporting (aligned to +/- strand):  ref base A (33/38);  new base T (2/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

CCCGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCGGGTTGTTCTGCCGGCGG  >  NC_002947/3490413‑3490698                                                                                                                                             |                                                                                                                                                   cccGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGcc                                                                                                                                             >  8:95543/1‑147 (MQ=255) cccGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGcc                                                                                                                                             >  5:175476/1‑147 (MQ=255) cccGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGcc                                                                                                                                             >  3:79370/1‑147 (MQ=255)   cGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAgg                                                                                                                                         <  8:238841/149‑1 (MQ=255)   cGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGAGCCGGCCTGTAACAGGCCCAgg                                                                                                                                         >  7:141703/1‑149 (MQ=255)      ccTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTTACAGGccggcgccg                                                                                                                                      <  2:132487/149‑8 (MQ=255)      ccTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTc                                                                                                                                       >  2:40660/1‑148 (MQ=255)       cTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGa                                                                                                                                     <  1:40660/149‑1 (MQ=255)       cTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGa                                                                                                                                     <  7:101780/149‑1 (MQ=255)         ggCCGCCGGAGCCGATCAGGGGGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAgg                                                                                                                                   <  6:241642/149‑1 (MQ=255)         ggCAGCAGGACCCGAACAGGGTCTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGCCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGATCTCGAACTCGCCCTTGACCCCGCCGCCGGCCTGAAACAGGCCCACGTCGAgg                                                                                                                                   >  8:16333/1‑149 (MQ=255)          gccgccGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAgg                                                                                                                                   <  5:24425/148‑1 (MQ=255)          gccgccGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAgg                                                                                                                                   <  7:193612/148‑1 (MQ=255)          gccgccGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAgg                                                                                                                                   <  3:172521/148‑1 (MQ=255)          gccgccGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCG‑CCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAgg                                                                                                                                   <  3:157365/147‑1 (MQ=255)                ggAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCa                                                                                                                            <  7:95543/149‑1 (MQ=255)                 gAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGTCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAg                                                                                                                           <  2:338693/149‑1 (MQ=255)                      gATCAGGGTGTGGGCTTCGTCGATGAACAGGATGAGTCGGGTGCAGGCTGCGCTTGCCATCTTCGATCACAGCGTTGAGGCGGTTATCGAACTCGCCATTGCCCCCGGCCCCGGCCTGTAACAGGCCAAGGTCGAGGGTTTGCAGGGc                                                                                                                        >  1:58809/1‑148 (MQ=255)                       aTCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAAc                                                                                                                     <  7:67935/149‑1 (MQ=255)                           gggTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTcc                                                                                                                 <  5:151513/149‑1 (MQ=255)                             gtgtGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCtt                                                                                                               <  2:293473/149‑1 (MQ=255)                             gtgtGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCtt                                                                                                               >  3:231981/1‑149 (MQ=255)                             gtgtGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCtt                                                                                                               >  5:261084/1‑149 (MQ=255)                             gtgtGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCtt                                                                                                               <  1:110611/149‑1 (MQ=255)                             gtgtGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCtt                                                                                                               <  4:231981/149‑1 (MQ=255)                              tgtgGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCtt                                                                                                               >  8:224253/1‑148 (MQ=255)                                tgGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCTAgg                                                                                                                                   >  1:343172/1‑126 (MQ=255)                                tgGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCGGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAg                                                                                                            >  7:312570/1‑149 (MQ=255)                                  ggCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGc                                                                                                           <  5:167295/148‑1 (MQ=255)                                   gCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCAc                                                                                                         <  6:175476/149‑1 (MQ=255)                                            aTGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCAc                                                                                                <  2:58809/149‑1 (MQ=255)                                                    gatga‑tCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCt                                                                                        >  8:254695/1‑149 (MQ=255)                                                    gatga‑tCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCt                                                                                        >  7:56348/1‑149 (MQ=255)                                                        a‑tCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGGCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTggg                                                                                     <  8:141703/148‑1 (MQ=255)                                                            gggTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAAGAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGAt                                                                                 <  7:16333/149‑1 (MQ=255)                                                            gggTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGa                                                                                  <  3:203578/148‑1 (MQ=255)                                                                  aGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAg                                                                           >  5:22592/1‑149 (MQ=255)                                                                    gCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAgcg                                                                         >  2:316189/1‑149 (MQ=255)                                                                         gcTTGACCTCTTCGATCACATCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCGGGCCTGTAACAGGCCCAGGTCGAGtgtg                                                                                                                                <  2:41354/89‑1 (MQ=255)                                                                         gcTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAgg                                                                    >  2:336622/1‑149 (MQ=255)                                                                         gcTTGACCTATTCGATCACAGCCTTGAGGCGGTTCTCCAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGgtg                                                                                                                                >  1:41354/1‑89 (MQ=255)                                                                          cTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGc                                                                   >  6:382104/1‑149 (MQ=255)                                                                             gACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCt                                                                 <  6:22592/148‑1 (MQ=255)                                                                               ccTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTc                                                               <  5:292439/148‑1 (MQ=255)                                                                               ccTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCg                                                              <  1:316189/149‑1 (MQ=255)                                                                                ctctTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGGCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCTATGTGCAGCGCCAGTCCTTCGa                                                             <  8:312570/149‑1 (MQ=255)                                                                                 tctTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGAc                                                            >  3:252006/1‑149 (MQ=255)                                                                                 tctTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGAc                                                            >  8:291592/1‑149 (MQ=255)                                                                                       aTCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCAcggc                                                      >  3:8548/1‑149 (MQ=255)                                                                                        tCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCAcggcg                                                     >  5:125914/1‑149 (MQ=255)                                                                                           caGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGtt                                                  <  5:189604/149‑1 (MQ=255)                                                                                             gCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGtttt                                                <  2:166040/149‑1 (MQ=255)                                                                                                  gAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCAcgg                                                       <  7:143075/137‑1 (MQ=255)                                                                                                  gAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCAcgg                                                       >  8:143075/1‑137 (MQ=255)                                                                                                     gCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCt                                                                                                                >  6:264605/1‑77 (MQ=255)                                                                                                     gCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCt                                                                                                                <  5:264605/77‑1 (MQ=255)                                                                                                     gCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGAccc                                        >  1:38573/1‑149 (MQ=255)                                                                                                     gCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGAccc                                        <  7:224253/149‑1 (MQ=255)                                                                                                       ggTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCg                                      >  5:24277/1‑149 (MQ=255)                                                                                                           ctcGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCtt                                  >  5:143722/1‑149 (MQ=255)                                                                                                             cGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCg                                >  7:33023/1‑149 (MQ=255)                                                                                                               aaCTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCTGACCCCGGCTTCGcc                              <  8:267206/149‑1 (MQ=255)                                                                                                               aaCTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGcc                              >  1:367510/1‑149 (MQ=255)                                                                                                                 cTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCg                            >  2:275105/1‑149 (MQ=255)                                                                                                                    gCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGt                           <  6:24277/147‑1 (MQ=255)                                                                                                                    gCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGa                         <  5:98384/149‑1 (MQ=255)                                                                                                                    gCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGa                         <  6:125914/149‑1 (MQ=255)                                                                                                                            ccccGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCggg                 <  2:38573/149‑1 (MQ=255)                                                                                                                            ccccGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCggg                 <  4:8548/149‑1 (MQ=255)                                                                                                                                 gcgcCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCGGgttgtt            <  1:336622/149‑1 (MQ=255)                                                                                                                                   ctgggCCTGTTACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCg                   <  5:314758/137‑1 (MQ=255)                                                                                                                                   ctgggCCTGTTACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCg                   >  6:314758/4‑140 (MQ=255)                                                                                                                                   ctgggCCTGTTACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCGGGTTGTTCt          >  6:242865/4‑149 (MQ=255)                                                                                                                                           gTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCGGGTTGTTCTGCcggcgg  >  6:246254/1‑149 (MQ=255)                                                                                                                                           gTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCGGGTTGTTCTGCcggcgg  >  6:282040/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                   CCCGGCCTGGCCGCCGGAGCCGATCAGGGTGTGGGCTTCGTCGATGAACAGGATGA‑TCGGGTGCAGGCTGCGCTTGACCTCTTCGATCACAGCCTTGAGGCGGTTCTCGAACTCGCCCTTGACCCCGGCGCCGGCCTGTAACAGGCCCAGGTCGAGGGTGTGCAGGGCAACGTCCTTGAGCACCGCCGGCACGTCACCCTGGGCGATGCGCAGCGCCAGGCCTTCGACCACGGCGGTTTTGCCGACCCCGGCTTCGCCCGTGAGGATCGGGTTGTTCTGCCGGCGG  >  NC_002947/3490413‑3490698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑