Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,491,233 A→T 7.3% L53Q (CTG→CAG)  clpV ← protein ClpV1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,491,2330AT 7.3% 163.9 / 2.8 55L53Q (CTG→CAG) clpVprotein ClpV1
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

ATGTGCGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGG  >  NC_002947/3491086‑3491378
                                                                                                                                                   |                                                                                                                                                 
aTGTGCGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCTCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTgcag                                                                                                                                                  <  8:1220/149‑1 (MQ=255)
     cggcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTgcagcagc                                                                                                                                              <  2:287637/148‑1 (MQ=255)
       gcgACAGCGCCGGGGTGGGGGTATTACCTTTCTGGAAGGTGCCCAGGCCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACTACCTGGTAGTGGCGCAGGATCGGCGCGAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                           >  2:135469/1‑149 (MQ=255)
       gcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                           <  2:53758/149‑1 (MQ=255)
            aGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                      <  1:94829/149‑1 (MQ=255)
            aGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTc                                                                                                                                      <  6:351094/149‑1 (MQ=255)
              cgcCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCa                                                                                                                                    >  5:345233/1‑149 (MQ=255)
                  ggggTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCCGTTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGGCGAGCAACTGCAGCAGCAGGTGCTCCACTTc                                                                                                                                <  2:3464/149‑1 (MQ=255)
                            tATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTaa                                                                                                                      >  5:86619/1‑149 (MQ=255)
                              ttACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATg                                                                                                                    <  6:86619/149‑1 (MQ=255)
                                aCCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATgg                                                                                                                   <  4:347282/148‑1 (MQ=255)
                                   tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCt                                                                                                               >  2:97966/1‑149 (MQ=255)
                                      cTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTcc                                                                                                             >  8:302341/1‑148 (MQ=255)
                                           aGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                          >  2:127764/1‑146 (MQ=255)
                                           aGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                          <  1:127764/146‑1 (MQ=255)
                                            ggTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAgg                                                                                                       >  5:337544/1‑148 (MQ=255)
                                              tGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                      <  7:32322/115‑1 (MQ=255)
                                              tGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                      >  8:32322/1‑115 (MQ=255)
                                                cccAGTGCGGTGACCAGCTGCGATGGCAGGCGTTCGGCGACGACCTGGTAGTGGAGCAGGATCGGCGCCAGGTCGCTGGCGTTGTTGGCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAg                                                                                                  <  5:179739/149‑1 (MQ=255)
                                                 ccAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAgg                                                                                                 <  5:178549/149‑1 (MQ=255)
                                                  cAGGGCGGTGACCAGCTGCGCTTGCTGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAggg                                                                                                <  7:170134/149‑1 (MQ=255)
                                                  cAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAggg                                                                                                >  6:212530/1‑149 (MQ=255)
                                                        ggTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGccgccg                                                                                          <  8:233854/149‑1 (MQ=255)
                                                                gCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATGGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCTCGCTTTCg                                                                                  <  8:202926/149‑1 (MQ=255)
                                                                         gCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTg                                                                         >  2:49841/1‑149 (MQ=255)
                                                                          cAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGc                                                                        <  7:302341/149‑1 (MQ=255)
                                                                           aGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCg                                                                       >  2:70179/1‑149 (MQ=255)
                                                                                     cgacgaCCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCg                                                             <  6:345233/149‑1 (MQ=255)
                                                                                                 aGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                  >  8:33003/1‑148 (MQ=255)
                                                                                                   tGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGaa                                               <  5:212530/149‑1 (MQ=255)
                                                                                                       gcAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                  >  2:196993/1‑142 (MQ=255)
                                                                                                       gcAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                  <  1:196993/142‑1 (MQ=255)
                                                                                                           gATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACt                                       >  6:205348/1‑149 (MQ=255)
                                                                                                               ggCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCa                                    <  1:97966/148‑1 (MQ=255)
                                                                                                                gcgcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAgg                                  >  5:345820/1‑149 (MQ=255)
                                                                                                                       gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcac                             >  2:345660/1‑147 (MQ=255)
                                                                                                                       gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACaa                           <  3:115004/149‑1 (MQ=255)
                                                                                                                          gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAgg                         <  1:70179/148‑1 (MQ=255)
                                                                                                                          gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGc                        <  6:337544/149‑1 (MQ=255)
                                                                                                                          gCTGTCGGTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAgg                         <  8:133452/148‑1 (MQ=255)
                                                                                                                            tGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGa                      >  6:154204/1‑149 (MQ=255)
                                                                                                                              tCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGAc                     <  1:49841/148‑1 (MQ=255)
                                                                                                                                   gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                            <  3:193777/136‑1 (MQ=255)
                                                                                                                                   gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                            >  4:193777/1‑136 (MQ=255)
                                                                                                                                   gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCAt                <  7:33003/148‑1 (MQ=255)
                                                                                                                                   gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATc               >  8:77174/1‑149 (MQ=255)
                                                                                                                                     tgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGCCTTCAGGTTCACAAGGCGCCCCCATCg              >  4:246417/1‑148 (MQ=255)
                                                                                                                                      gtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGt             >  1:362626/1‑148 (MQ=255)
                                                                                                                                        ggAGCACCTGCTGCTGCAGTTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAg          >  6:308479/2‑149 (MQ=255)
                                                                                                                                        cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGc                                                       <  6:195571/104‑1 (MQ=255)
                                                                                                                                        cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGc                                                       >  5:195571/1‑104 (MQ=255)
                                                                                                                                        cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTGCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAg          <  7:287439/149‑1 (MQ=255)
                                                                                                                                          aGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                  >  2:135272/1‑107 (MQ=255)
                                                                                                                                          aGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCAGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAgg         >  6:219347/1‑148 (MQ=255)
                                                                                                                                             aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtg     <  5:205348/149‑1 (MQ=255)
                                                                                                                                             aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGGGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtg     <  1:345660/149‑1 (MQ=255)
                                                                                                                                               cTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtgtg   <  5:154204/149‑1 (MQ=255)
                                                                                                                                                  cagcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTgg  >  1:38552/1‑147 (MQ=255)
                                                                                                                                                   agcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCTAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTgg  >  8:138241/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATGTGCGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGG  >  NC_002947/3491086‑3491378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑