Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,491,236 A→T 7.3% L52Q (CTG→CAG)  clpV ← protein ClpV1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,491,2360AT 7.3% 162.5 / 2.8 55L52Q (CTG→CAG) clpVprotein ClpV1
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

CGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGA  >  NC_002947/3491091‑3491382
                                                                                                                                                 |                                                                                                                                                  
cggcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTgcagcagc                                                                                                                                                  <  2:287637/148‑1 (MQ=255)
  gcgACAGCGCCGGGGTGGGGGTATTACCTTTCTGGAAGGTGCCCAGGCCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACTACCTGGTAGTGGCGCAGGATCGGCGCGAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                               >  2:135469/1‑149 (MQ=255)
  gcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                               <  2:53758/149‑1 (MQ=255)
       aGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                          <  1:94829/149‑1 (MQ=255)
       aGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTc                                                                                                                                          <  6:351094/149‑1 (MQ=255)
         cgcCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCa                                                                                                                                        >  5:345233/1‑149 (MQ=255)
             ggggTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCCGTTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGGCGAGCAACTGCAGCAGCAGGTGCTCCACTTc                                                                                                                                    <  2:3464/149‑1 (MQ=255)
                       tATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTaa                                                                                                                          >  5:86619/1‑149 (MQ=255)
                         ttACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATg                                                                                                                        <  6:86619/149‑1 (MQ=255)
                           aCCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATgg                                                                                                                       <  4:347282/148‑1 (MQ=255)
                              tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCt                                                                                                                   >  2:97966/1‑149 (MQ=255)
                                 cTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTcc                                                                                                                 >  8:302341/1‑148 (MQ=255)
                                      aGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                              <  1:127764/146‑1 (MQ=255)
                                      aGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                              >  2:127764/1‑146 (MQ=255)
                                       ggTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAgg                                                                                                           >  5:337544/1‑148 (MQ=255)
                                         tGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                          <  7:32322/115‑1 (MQ=255)
                                         tGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                          >  8:32322/1‑115 (MQ=255)
                                           cccAGTGCGGTGACCAGCTGCGATGGCAGGCGTTCGGCGACGACCTGGTAGTGGAGCAGGATCGGCGCCAGGTCGCTGGCGTTGTTGGCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAg                                                                                                      <  5:179739/149‑1 (MQ=255)
                                            ccAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAgg                                                                                                     <  5:178549/149‑1 (MQ=255)
                                             cAGGGCGGTGACCAGCTGCGCTTGCTGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAggg                                                                                                    <  7:170134/149‑1 (MQ=255)
                                             cAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAggg                                                                                                    >  6:212530/1‑149 (MQ=255)
                                                   ggTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGccgccg                                                                                              <  8:233854/149‑1 (MQ=255)
                                                           gCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATGGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCTCGCTTTCg                                                                                      <  8:202926/149‑1 (MQ=255)
                                                                    gCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTg                                                                             >  2:49841/1‑149 (MQ=255)
                                                                     cAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGc                                                                            <  7:302341/149‑1 (MQ=255)
                                                                      aGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCg                                                                           >  2:70179/1‑149 (MQ=255)
                                                                                cgacgaCCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCg                                                                 <  6:345233/149‑1 (MQ=255)
                                                                                            aGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                      >  8:33003/1‑148 (MQ=255)
                                                                                              tGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGaa                                                   <  5:212530/149‑1 (MQ=255)
                                                                                                  gcAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                      <  1:196993/142‑1 (MQ=255)
                                                                                                  gcAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                      >  2:196993/1‑142 (MQ=255)
                                                                                                      gATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACt                                           >  6:205348/1‑149 (MQ=255)
                                                                                                          ggCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCa                                        <  1:97966/148‑1 (MQ=255)
                                                                                                           gcgcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAgg                                      >  5:345820/1‑149 (MQ=255)
                                                                                                                  gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcac                                 >  2:345660/1‑147 (MQ=255)
                                                                                                                  gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACaa                               <  3:115004/149‑1 (MQ=255)
                                                                                                                     gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAgg                             <  1:70179/148‑1 (MQ=255)
                                                                                                                     gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGc                            <  6:337544/149‑1 (MQ=255)
                                                                                                                     gCTGTCGGTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAgg                             <  8:133452/148‑1 (MQ=255)
                                                                                                                       tGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGa                          >  6:154204/1‑149 (MQ=255)
                                                                                                                         tCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGAc                         <  1:49841/148‑1 (MQ=255)
                                                                                                                              gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                                >  4:193777/1‑136 (MQ=255)
                                                                                                                              gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                                <  3:193777/136‑1 (MQ=255)
                                                                                                                              gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCAt                    <  7:33003/148‑1 (MQ=255)
                                                                                                                              gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATc                   >  8:77174/1‑149 (MQ=255)
                                                                                                                                tgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGCCTTCAGGTTCACAAGGCGCCCCCATCg                  >  4:246417/1‑148 (MQ=255)
                                                                                                                                 gtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGt                 >  1:362626/1‑148 (MQ=255)
                                                                                                                                   ggAGCACCTGCTGCTGCAGTTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAg              >  6:308479/2‑149 (MQ=255)
                                                                                                                                   cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGc                                                           <  6:195571/104‑1 (MQ=255)
                                                                                                                                   cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGc                                                           >  5:195571/1‑104 (MQ=255)
                                                                                                                                   cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTGCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAg              <  7:287439/149‑1 (MQ=255)
                                                                                                                                     aGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                      >  2:135272/1‑107 (MQ=255)
                                                                                                                                     aGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCAGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAgg             >  6:219347/1‑148 (MQ=255)
                                                                                                                                        aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtg         <  5:205348/149‑1 (MQ=255)
                                                                                                                                        aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGGGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtg         <  1:345660/149‑1 (MQ=255)
                                                                                                                                          cTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtgtg       <  5:154204/149‑1 (MQ=255)
                                                                                                                                             cagcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTgg      >  1:38552/1‑147 (MQ=255)
                                                                                                                                              agcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCTAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTgg      >  8:138241/1‑146 (MQ=255)
                                                                                                                                               gcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGa  >  7:153702/1‑149 (MQ=255)
                                                                                                                                               gcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGa  >  2:152099/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGGCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGA  >  NC_002947/3491091‑3491382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑