Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,491,241	G→T	6.6%	H50Q (CAC→CAA)	clpV ←	protein ClpV1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,491,241	0	G	T	6.6%	196.0 / 3.4	61	H50Q (CAC→CAA)	clpV	protein ClpV1
Reads supporting (aligned to +/- strand):  ref base G (30/27);  new base T (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCG  >  NC_002947/3491093‑3491387                                                                                                                                                     |                                                                                                                                                   gcgACAGCGCCGGGGTGGGGGTATTACCTTTCTGGAAGGTGCCCAGGCCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACTACCTGGTAGTGGCGCAGGATCGGCGCGAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                                    >  2:135469/1‑149 (MQ=255) gcgACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAgg                                                                                                                                                    <  2:53758/149‑1 (MQ=255)      aGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                               <  1:94829/149‑1 (MQ=255)      aGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTc                                                                                                                                               <  6:351094/149‑1 (MQ=255)        cgcCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCa                                                                                                                                             >  5:345233/1‑149 (MQ=255)            ggggTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCCGTTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGGCGAGCAACTGCAGCAGCAGGTGCTCCACTTc                                                                                                                                         <  2:3464/149‑1 (MQ=255)                      tATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTaa                                                                                                                               >  5:86619/1‑149 (MQ=255)                        ttACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATg                                                                                                                             <  6:86619/149‑1 (MQ=255)                          aCCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATgg                                                                                                                            <  4:347282/148‑1 (MQ=255)                             tttCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCt                                                                                                                        >  2:97966/1‑149 (MQ=255)                                cTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTcc                                                                                                                      >  8:302341/1‑148 (MQ=255)                                     aGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                                   <  1:127764/146‑1 (MQ=255)                                     aGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGAc                                                                                                                   >  2:127764/1‑146 (MQ=255)                                      ggTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAgg                                                                                                                >  5:337544/1‑148 (MQ=255)                                        tGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                               <  7:32322/115‑1 (MQ=255)                                        tGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCACCTGCTGCTGCAGTTGCTc                                                                                                                                               >  8:32322/1‑115 (MQ=255)                                          cccAGTGCGGTGACCAGCTGCGATGGCAGGCGTTCGGCGACGACCTGGTAGTGGAGCAGGATCGGCGCCAGGTCGCTGGCGTTGTTGGCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAg                                                                                                           <  5:179739/149‑1 (MQ=255)                                           ccAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAgg                                                                                                          <  5:178549/149‑1 (MQ=255)                                            cAGGGCGGTGACCAGCTGCGCTTGCTGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAggg                                                                                                         <  7:170134/149‑1 (MQ=255)                                            cAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAggg                                                                                                         >  6:212530/1‑149 (MQ=255)                                                  ggTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGccgccg                                                                                                   <  8:233854/149‑1 (MQ=255)                                                          gCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATGGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCTCGCTTTCg                                                                                           <  8:202926/149‑1 (MQ=255)                                                                   gCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTg                                                                                  >  2:49841/1‑149 (MQ=255)                                                                    cAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGc                                                                                 <  7:302341/149‑1 (MQ=255)                                                                     aGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCg                                                                                >  2:70179/1‑149 (MQ=255)                                                                               cgacgaCCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCg                                                                      <  6:345233/149‑1 (MQ=255)                                                                                           aGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                           >  8:33003/1‑148 (MQ=255)                                                                                             tGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGaa                                                        <  5:212530/149‑1 (MQ=255)                                                                                                 gcAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                           <  1:196993/142‑1 (MQ=255)                                                                                                 gcAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                           >  2:196993/1‑142 (MQ=255)                                                                                                     gATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACt                                                >  6:205348/1‑149 (MQ=255)                                                                                                         ggCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCa                                             <  1:97966/148‑1 (MQ=255)                                                                                                          gcgcCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAgg                                           >  5:345820/1‑149 (MQ=255)                                                                                                                 gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcac                                      >  2:345660/1‑147 (MQ=255)                                                                                                                 gTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACaa                                    <  3:115004/149‑1 (MQ=255)                                                                                                                    gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAgg                                  <  1:70179/148‑1 (MQ=255)                                                                                                                    gCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGc                                 <  6:337544/149‑1 (MQ=255)                                                                                                                    gCTGTCGGTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAgg                                  <  8:133452/148‑1 (MQ=255)                                                                                                                      tGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGa                               >  6:154204/1‑149 (MQ=255)                                                                                                                        tCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGAc                              <  1:49841/148‑1 (MQ=255)                                                                                                                             gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                                     >  4:193777/1‑136 (MQ=255)                                                                                                                             gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTcaca                                     <  3:193777/136‑1 (MQ=255)                                                                                                                             gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCAt                         <  7:33003/148‑1 (MQ=255)                                                                                                                             gttgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATc                        >  8:77174/1‑149 (MQ=255)                                                                                                                               tgtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGCCTTCAGGTTCACAAGGCGCCCCCATCg                       >  4:246417/1‑148 (MQ=255)                                                                                                                                gtCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGt                      >  1:362626/1‑148 (MQ=255)                                                                                                                                  ggAGCACCTGCTGCTGCAGTTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAg                   >  6:308479/2‑149 (MQ=255)                                                                                                                                  cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGc                                                                <  6:195571/104‑1 (MQ=255)                                                                                                                                  cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGc                                                                >  5:195571/1‑104 (MQ=255)                                                                                                                                  cGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTGCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAg                   <  7:287439/149‑1 (MQ=255)                                                                                                                                    aGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGc                                                           >  2:135272/1‑107 (MQ=255)                                                                                                                                    aGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCAGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAgg                  >  6:219347/1‑148 (MQ=255)                                                                                                                                       aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtg              <  5:205348/149‑1 (MQ=255)                                                                                                                                       aaCTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGGGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtg              <  1:345660/149‑1 (MQ=255)                                                                                                                                         cTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAtgtg            <  5:154204/149‑1 (MQ=255)                                                                                                                                            cagcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTgg           >  1:38552/1‑147 (MQ=255)                                                                                                                                             agcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCTAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTgg           >  8:138241/1‑146 (MQ=255)                                                                                                                                              gcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGa       >  2:152099/1‑149 (MQ=255)                                                                                                                                              gcagcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGa       >  7:153702/1‑149 (MQ=255)                                                                                                                                                 gcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACgg    >  8:108115/1‑149 (MQ=255)                                                                                                                                                 gcagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACgg    >  6:195499/1‑149 (MQ=255)                                                                                                                                                  cagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGgc   >  6:216348/1‑149 (MQ=255)                                                                                                                                                  cagGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGATGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGCCGgc   >  4:220133/1‑149 (MQ=255)                                                                                                                                                    ggTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAAAGTGGAAGACGgcg  >  3:166765/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCGACAGCGCCGGGGTGCGGGTATTACCTTTCTTGAAGGTGCCCAGGGCGGTGACCAGCTGCGCTTGCAGGCGTTCGGCGACGACCTGGTAGTGGCGCAGGATCGGCGCCAGGTCGCTGTCGTTGTTGTCGAGCAACTGCAGCAGCAGGTGCTCCACTTCCACCTCGTAATGGTGCTCCGACAGGCACAGGGCCGCCGCGCTTTCGGTGGCCGTGCGGCTGGTTTCGTTGAGCTTGGCGAACAGAGACTTCAGGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCG  >  NC_002947/3491093‑3491387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑