Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,525,858 T→C 7.6% L102L (TTG→CTG) ‡ lexA‑II → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,525,8580TC 7.6% 180.6 / 4.7 67L102L (TTG→CTG) ‡lexA‑IItranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base C (2/3);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

TTACATCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGTT  >  NC_002947/3525716‑3526004
                                                                                                                                              |                                                                                                                                                  
ttACATCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTtgctgc                                                                                                                                              <  8:191302/149‑1 (MQ=255)
    aTCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGa                                                                                                                                          >  3:214337/1‑149 (MQ=255)
       gATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGCAACTGCTCgtgga                                                                                                                                       <  7:365478/149‑5 (MQ=255)
           tCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGc                                                                                                                                   >  5:143109/1‑149 (MQ=255)
           tCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGCAACTGCTCgtggatgc                                                                                                                                    <  5:18150/148‑8 (MQ=255)
             aCGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTTCTGGGCCATGTGGCGGCGGGTGCCCCAATCGGCCCGTACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGcc                                                                                                                                  <  2:273772/148‑1 (MQ=255)
               gCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGt                                                                                                                               >  6:178670/1‑149 (MQ=255)
               gCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGt                                                                                                                               >  1:336483/1‑149 (MQ=255)
                ccAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCCCGAGCAGATGCTGCTCGACCCCAGCCTGtt                                                                                                                              >  5:254824/1‑149 (MQ=255)
                    aCCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCTCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTccgc                                                                                                                          >  2:264245/1‑149 (MQ=255)
                     ccAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTccgcc                                                                                                                         >  5:352598/1‑149 (MQ=255)
                        ggCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACCAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTa                                                                                                                      >  4:326405/1‑149 (MQ=255)
                         gcgcGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTAc                                                                                                                     >  7:102559/1‑149 (MQ=255)
                         gcgcGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTAc                                                                                                                     >  5:376914/1‑149 (MQ=255)
                              gggCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCg                                                                                                                <  8:102559/149‑1 (MQ=255)
                               ggCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGa                                                                                                               <  2:168533/149‑1 (MQ=255)
                               ggCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGa                                                                                                               <  5:178670/149‑1 (MQ=255)
                                  aTACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTtgctg                                                                                                                                               <  7:169535/114‑1 (MQ=255)
                                  aTACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTtgctg                                                                                                                                               >  8:169535/1‑114 (MQ=255)
                                   tACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCCGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTAc                                                                                                           <  7:105521/149‑1 (MQ=255)
                                     cGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTtgctgc                                                                                                                                              <  6:334007/112‑1 (MQ=255)
                                     cGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTtgctgc                                                                                                                                              >  5:334007/1‑112 (MQ=255)
                                      gCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctg                                                                                                        >  4:245753/1‑149 (MQ=255)
                                        cTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctgct                                                                                                      >  3:87075/1‑149 (MQ=255)
                                        cTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctgct                                                                                                      >  2:251084/1‑149 (MQ=255)
                                         tGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACctgct                                                                                                      >  6:225658/1‑148 (MQ=255)
                                            ccGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAg                                                                                                  <  1:65717/149‑1 (MQ=255)
                                                           ggccggAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTc                                                                                    >  5:256558/1‑148 (MQ=255)
                                                           ggccggAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCAT‑CACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCga                                                                                  <  4:274758/149‑1 (MQ=255)
                                                            gccggAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCga                                                                                  <  6:352598/149‑1 (MQ=255)
                                                            gccggAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCga                                                                                  <  6:86786/149‑1 (MQ=255)
                                                                gAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCgatgat                                                                              <  3:245753/149‑1 (MQ=255)
                                                                   aTCCTCGAAATCCCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATcga                                                                           <  7:71014/149‑1 (MQ=255)
                                                                   aTCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATcga                                                                           <  1:197171/149‑1 (MQ=255)
                                                                       tCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCgacgac                                                                       >  7:106734/1‑149 (MQ=255)
                                                                       tCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACACga                                                                                  <  1:349595/138‑1 (MQ=255)
                                                                       tCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACACga                                                                                  >  2:349595/1‑138 (MQ=255)
                                                                                ccGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCttt                                                              >  6:153892/1‑149 (MQ=255)
                                                                                ccGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCAGGACCTGGGAATCCACGAGCAGTTGCTGCTCGCCCCCAGCCTGTTCCGACGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCttt                                                              >  6:208067/1‑149 (MQ=255)
                                                                                 cGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATcgacga                                                                        <  8:254593/138‑1 (MQ=255)
                                                                                    gCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACg                                                          <  4:214337/149‑1 (MQ=255)
                                                                                     cTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACg                                                          <  3:128886/148‑1 (MQ=255)
                                                                                         gCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGAc                                                     <  6:256558/149‑1 (MQ=255)
                                                                                          ccAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGAcc                                                    >  4:3311/1‑149 (MQ=255)
                                                                                           cAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCt                                                   <  6:46659/149‑1 (MQ=255)
                                                                                                ggcggcGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                              <  8:106734/149‑1 (MQ=255)
                                                                                                 gcggcgGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                              <  2:336483/148‑1 (MQ=255)
                                                                                                  cggcggGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGc                                            <  3:326405/149‑1 (MQ=255)
                                                                                                     cggGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATc                                         <  6:376914/149‑1 (MQ=255)
                                                                                                       ggTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCt                                       <  5:225658/149‑1 (MQ=255)
                                                                                                             cccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                              >  7:189020/1‑136 (MQ=255)
                                                                                                             cccATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCg                                              <  8:189020/136‑1 (MQ=255)
                                                                                                                    gCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGAc                          >  8:212701/1‑149 (MQ=255)
                                                                                                                       cGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGcc                       >  2:265716/1‑149 (MQ=255)
                                                                                                                        ggACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGccc                      <  1:251084/149‑1 (MQ=255)
                                                                                                                              gggCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACg                >  4:38851/1‑149 (MQ=255)
                                                                                                                               ggCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACgg               >  1:244078/1‑149 (MQ=255)
                                                                                                                                 cATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGc             <  1:264245/149‑1 (MQ=255)
                                                                                                                                    ccACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGc             <  5:338704/146‑1 (MQ=255)
                                                                                                                                    ccACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGc             >  6:338704/1‑146 (MQ=255)
                                                                                                                                     cACGAGCAGTTGCTGCTCGACCGCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAAt         >  7:60352/1‑149 (MQ=255)
                                                                                                                                      tcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCt                                       >  2:18858/2‑118 (MQ=255)
                                                                                                                                      tcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCt                                       <  1:18858/117‑1 (MQ=255)
                                                                                                                                      tcGAGCAGCAACTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATc        >  6:332895/2‑149 (MQ=255)
                                                                                                                                      aCGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGAACGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATc        >  1:305101/1‑149 (MQ=255)
                                                                                                                                         aGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTg     <  1:265716/149‑1 (MQ=255)
                                                                                                                                            aGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGtt  <  6:143109/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TTACATCGATGTCACGCCAAACCAGGCGCGGGGCATACGCCTGGCCGAGCCGCTGCGCCGGCCGGAAATCCTCGAAATACCCGTGCTGGGCCAGGTGGCGGCGGGTGCCCCCATCGGCCCGGACCTGGGCATCCACGAGCAGTTGCTGCTCGACCCCAGCCTGTTCCGCCGTACCCCCGACTACCTGCTGAAGGTGCGGGGCGACTCGATGATCGACGACGGCATCTTTGACGGTGACCTGGTCGGCATCCTGCAACAGGCCGACGCCCGCGACGGGCAAATCGTGGTT  >  NC_002947/3525716‑3526004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑