Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,610,270 G→T 10.0% intergenic (‑38/+18) PP_3183 ← / ← mcoA SCO1/SenC family protein/Mn(II) copper oxidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,610,2700GT10.0% 147.2 / 7.0 50intergenic (‑38/+18)PP_3183/mcoASCO1/SenC family protein/Mn(II) copper oxidase A
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base T (2/3);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GAGGCCGCTGCTTCGGCCACCGTTCGTTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTAGTCGTGG  >  NC_002947/3610129‑3610415
                                                                                                                                             |                                                                                                                                                 
gagGCCGCTGCTTCGGCCACCGTTCGTTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCCATGCGGccc                                                                                                                                            <  3:299107/149‑1 (MQ=255)
       ctgctTCGGCCACCGTTCGTTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCCATGCGGcccccatcag                                                                                                                                     <  1:73241/149‑7 (MQ=255)
          ctTCGGCCACCGTTCGTTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGca                                                                                                                                   <  1:229551/148‑1 (MQ=255)
            tCGGCCACCGTTCGGTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACCACGCCAGTACCAGCAGGAATCCCCAGGAGCCGGACATGATGCGGTCCTCGTGGGTGGGCTGATGGCGGCCGCATGCCGGCCCCGCTATGcact                                                                                                                                 >  2:97219/1‑148 (MQ=255)
               gCCACCGTTCGTTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGcactcact                                                                                                                             >  6:63919/1‑149 (MQ=255)
                              ggTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGgcg                                                                                                              >  4:180939/1‑149 (MQ=255)
                                ttGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGGATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCt                                                                                                            <  7:303502/149‑1 (MQ=255)
                                         ccAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTAtgt                                                                                                                      >  4:354243/1‑130 (MQ=255)
                                         ccAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTAtgt                                                                                                                      <  3:354243/130‑1 (MQ=255)
                                           aGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTg                                                                                                 >  4:82234/1‑149 (MQ=255)
                                            gAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCGTGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTgg                                                                                                <  3:38235/149‑1 (MQ=255)
                                             aaCGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGc                                                                                               <  1:178875/149‑1 (MQ=255)
                                                ggAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCACATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGAcc                                                                                              >  6:333684/1‑147 (MQ=255)
                                                  aaTGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGt                                                                                                                          >  6:241151/1‑117 (MQ=255)
                                                  aaTGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGt                                                                                                                          <  5:241151/117‑1 (MQ=255)
                                                      cTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTc                                                                                       <  5:63919/148‑1 (MQ=255)
                                                              gAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCAcc                                                                              >  7:180373/1‑149 (MQ=255)
                                                                 caacGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGAGCTGTCACCGTCTGGCCCACCGTCGATTTCACCGTg                                                                           >  7:346866/1‑149 (MQ=255)
                                                                  aacGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGa                                                                          >  2:171135/1‑149 (MQ=255)
                                                                               gcagGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGt                                                             >  4:102260/1‑149 (MQ=255)
                                                                                   gAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCATCGTGACCGTCGCTGGCGTGGcc                                                         >  1:386324/1‑149 (MQ=255)
                                                                                        gCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGc                                                     <  3:180939/148‑1 (MQ=255)
                                                                                        gCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGc                                                     >  7:127154/1‑148 (MQ=255)
                                                                                         ccATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGcc                                                    >  8:167697/1‑148 (MQ=255)
                                                                                         ccATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGcc                                                    >  6:72818/1‑148 (MQ=255)
                                                                                         ccATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGcc                                                    >  5:56055/1‑148 (MQ=255)
                                                                                               gCCGGGCATGCTGCGGTACTCGTGGGTGGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGAGTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCg                                             <  4:280412/149‑1 (MQ=255)
                                                                                               gCCGGACATGATGCGGTCCTCGTGGGTGGGCTGATGGGGGCCGCAGGGCGGCCCCGCTGTGCACTCACTTGTAGGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCg                                             <  1:97219/149‑1 (MQ=255)
                                                                                                ccGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTGTGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCgg                                            >  6:321969/1‑149 (MQ=255)
                                                                                                   ggCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCggtgg                                         <  5:72818/149‑1 (MQ=255)
                                                                                                   ggCATGATGCGGTCCTCGTGGGTTGGCTGAAGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCggtgg                                         >  1:33331/1‑149 (MQ=255)
                                                                                                     cATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCggtggtg                                       <  6:56055/149‑1 (MQ=255)
                                                                                                             ggTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTg                                                                 <  8:261769/115‑1 (MQ=255)
                                                                                                             ggTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTg                                                                 >  7:261769/1‑115 (MQ=255)
                                                                                                              gTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGc                               >  8:183626/1‑148 (MQ=255)
                                                                                                                 cTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTAcc                           >  8:12180/1‑149 (MQ=255)
                                                                                                                  tCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTAccc                          >  2:288990/1‑149 (MQ=255)
                                                                                                                     tGGGTTGGCTGATGGGGGCCACATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGACCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGcc                       <  5:333684/149‑1 (MQ=255)
                                                                                                                             cTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCggtggtg                                       <  4:273850/125‑1 (MQ=255)
                                                                                                                              tGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAAc                    >  2:53844/1‑143 (MQ=255)
                                                                                                                              tGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAAc                    <  1:53844/143‑1 (MQ=255)
                                                                                                                              tGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGccc              >  5:307215/1‑149 (MQ=255)
                                                                                                                              aaagcggggCCGCCATGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGccc              >  6:76478/6‑149 (MQ=255)
                                                                                                                                aTGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGccccc            <  3:102260/149‑1 (MQ=255)
                                                                                                                                   ggggCCGCCATGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCg           >  7:311920/1‑147 (MQ=255)
                                                                                                                                   ggggCCGCCATGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTa         <  5:76478/149‑1 (MQ=255)
                                                                                                                                    gggCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCATCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTAg        <  2:386324/149‑1 (MQ=255)
                                                                                                                                        cGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTAGTCGt    <  7:183626/149‑1 (MQ=255)
                                                                                                                                         gCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTAGTCGTg   >  8:57508/1‑149 (MQ=255)
                                                                                                                                          cATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGCCCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTAGTCGTgg  >  4:231502/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
GAGGCCGCTGCTTCGGCCACCGTTCGTTGGGGTTGCAACGGCCAGAACGGAATGCTGGCGGCGAACAACGCCAGTACCAGCAGGAATCGCCATGAGCCGGGCATGATGCGGTCCTCGTGGGTTGGCTGATGGGGGCCGCATGGCGGCCCCGCTTTGCACTCACTTGTATGTGACTGGCGCTGTCACCGTCTGGCCCAGCGTCGATTTCACCGTGACCGTCGCTGGCGTGGCCGGGCCAAAGCCGGTGGTGGTCACCGCTACCCGCCAACGGGCCCCCGTAGTCGTGG  >  NC_002947/3610129‑3610415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑