Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,614,722	A→C	5.3%	T199P (ACC→CCC)	pet →	protein Pet18

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,614,722	0	A	C	5.3%	237.6 / 2.4	85	T199P (ACC→CCC)	pet	protein Pet18
Reads supporting (aligned to +/- strand):  ref base A (42/38);  new base C (3/2);  total (45/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

GAAACCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAG  >  NC_002947/3614574‑3614865                                                                                                                                                       |                                                                                                                                                 gAAACCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCa                                                                                                                                                  >  1:92828/1‑149 (MQ=255)  aaaCCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCAc                                                                                                                                                 >  1:194344/1‑149 (MQ=255)   aaCCGATGCCGGACAACTTCGTCCATTCCGAGTGGATTACCCTGCATGACAACCCTGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGACGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACAAGCGCAcc                                                                                                                                                >  7:346128/1‑149 (MQ=255)     ccGATGCCGTACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAAACCCGCATTCAAGGCATTCGT‑CGCTTTCCTGCAGTCAGAACAGGACCGCGACCCACCTCAGGAGGACGCTTTT‑GACAGTGACTTCAACCACCGCACCGt                                                                                                                                              >  6:233284/1‑149 (MQ=255)       gATGCCGGAGTACTTCGTGCATGCCGTGTGGATTACCCTGCATGACAAGGCGGCATTCGAGGCATTCGT‑CGCTTTCCTGCAGGCCGATCTGGACCTCGTCGGCCCGCAGGAGGCCGCTTTG‑GGAAGTGACTTCTACCAGCGCAGCGTgg                                                                                                                                            <  2:340009/149‑1 (MQ=255)       gATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTgg                                                                                                                                            >  3:96/1‑149 (MQ=255)            cGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTg                                                                                                                                       >  3:33325/1‑149 (MQ=255)             ggACTACTTCGTCCATGCCGAGTGGATTAACCTGCATGACAACCCTGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCTAACTGGAACGCGTCGGCCCGCAGGATGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTgg                                                                                                                                      <  8:346128/149‑1 (MQ=255)                 aaCTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCt                                                                                                                                  >  5:342553/1‑149 (MQ=255)                   cTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCCACGGTGCGCTggtagaag                                                                                                                                <  3:257/149‑7 (MQ=255)                   cTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCCACGGTGCGCTggtagaag                                                                                                                                <  4:300766/149‑7 (MQ=255)                   cTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTgg                                                                                                                                >  5:126572/1‑149 (MQ=255)                          cATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAg                                                                                                                                    >  2:64461/1‑138 (MQ=255)                          cATGCCGAGTGGAATAACCTTCATGCCAACCCGGCATTCCAGGCATTCGT‑GGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCATGATGGCGCTTTGTGCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAg                                                                                                                                    <  1:64461/139‑1 (MQ=255)                                gAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGc                                                                                                                  >  8:100336/1‑149 (MQ=255)                                 aGTGGAATACCCTGCATGACAACCCGGCATTCCAGGCAATCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAACTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCt                                                                                                                 >  2:116789/1‑149 (MQ=255)                                  gTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCtg                                                                                                                <  3:290899/149‑1 (MQ=255)                                  gTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCtg                                                                                                                <  6:66616/149‑1 (MQ=255)                                   tGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCtgt                                                                                                               <  7:189400/149‑1 (MQ=255)                                   tGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCtgt                                                                                                               <  6:193958/149‑1 (MQ=255)                                    ggATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCtgtg                                                                                                              >  1:52309/1‑149 (MQ=255)                                      aTTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTGTG‑GCAAGTGACTTCTACCAGCGCCCCGTGGCGCTGCAGCTGG‑CGTTCTTCGCTGCTGTTTa                                                                                                            >  6:368013/1‑149 (MQ=255)                                          cccTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCg                                                                                                        >  1:329677/1‑149 (MQ=255)                                           ccTGCATGACAACCCGGCATTCCAGGCATTCGTACGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCg                                                                                                        >  1:46021/1‑149 (MQ=255)                                            cTGCATGTCAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCg                                                                                                        >  5:211090/1‑147 (MQ=255)                                            cTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGa                                                                                                      <  4:33325/149‑1 (MQ=255)                                               cATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAg                                                                                                     <  2:52309/147‑1 (MQ=255)                                                 tGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTgg                                                                                                                                            >  4:19058/1‑107 (MQ=255)                                                 tGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTgg                                                                                                                                            <  3:19058/107‑1 (MQ=255)                                                   aCAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCaa                                                                                                <  2:92828/148‑1 (MQ=255)                                                     aaCCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGGCGAACTGGACCGCGTGGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCg                                                                                                                      <  8:340455/124‑1 (MQ=255)                                                     aaCCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCCCCGTGGCGCTGGCGCTGG‑CTTTCGTCGAGGCTGTGTACCCGGAGGGCAAAt                                                                                              >  8:341300/1‑148 (MQ=255)                                                     aaCCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCg                                                                                                                      >  7:340455/1‑124 (MQ=255)                                                     aaCCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATg                                                                                             <  2:46021/149‑1 (MQ=255)                                                      aCCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGa                                                                                            <  5:33176/149‑1 (MQ=255)                                                        ccGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGc                                                                                          <  2:339503/149‑1 (MQ=255)                                                        ccGGCATTCCAGGCACTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAg                                                                                           >  8:222865/1‑148 (MQ=255)                                                                 cAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATc                                                                                 >  3:341641/1‑149 (MQ=255)                                                                  aGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCt                                                                                >  5:182062/1‑149 (MQ=255)                                                                    gCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTc                                                                              <  8:317291/149‑1 (MQ=255)                                                                      aTTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGa                                                                            <  6:126572/149‑1 (MQ=255)                                                                         cgt‑cgCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAgcg                                                                         >  7:297498/1‑149 (MQ=255)                                                                         cgt‑cgCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAgc                                                                          <  4:140884/148‑1 (MQ=255)                                                                           t‑cgCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                       >  7:270401/1‑149 (MQ=255)                                                                           t‑cgCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                       >  4:331420/1‑149 (MQ=255)                                                                           t‑cgCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGcc                                                                       <  7:100336/149‑1 (MQ=255)                                                                                tttCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTc                                                                     >  7:138961/1‑147 (MQ=255)                                                                                  tCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAg                                                                  <  6:211090/148‑1 (MQ=255)                                                                                   ccTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGATCGCCTCAAgg                                                                 <  6:342553/148‑1 (MQ=255)                                                                                       cAGGCCGCACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCg                                                              <  7:341300/147‑1 (MQ=255)                                                                                        aGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTg                                                           <  8:138961/149‑1 (MQ=255)                                                                                                 tGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccacgcca                                                   >  4:144653/1‑148 (MQ=255)                                                                                                 tGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgccacgcca                                                   >  3:351826/1‑148 (MQ=255)                                                                                                   gACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CGTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACaa                                                <  4:96/149‑1 (MQ=255)                                                                                                       gcgTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGa                                            <  6:182062/149‑1 (MQ=255)                                                                                                         gTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAAc                                          <  2:329677/149‑1 (MQ=255)                                                                                                              cccGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATGTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTa                                     >  2:216482/1‑149 (MQ=255)                                                                                                               ccGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCACGGCCGCTGCCACGCCACAATGGAACCGCTAc                                    >  8:167549/1‑149 (MQ=255)                                                                                                               ccGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTAc                                    >  6:142259/1‑149 (MQ=255)                                                                                                               ccGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTAc                                    <  7:222865/149‑1 (MQ=255)                                                                                                               ccGCAGGAAGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTAc                                    >  2:280175/1‑149 (MQ=255)                                                                                                                  cAGGAGGCCGCTTTG‑TCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTa                                 <  6:133810/149‑1 (MQ=255)                                                                                                                  cAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTa                                 >  7:20506/1‑149 (MQ=255)                                                                                                                      aggCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGAcc                             <  4:351826/149‑1 (MQ=255)                                                                                                                        gCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCAt                           <  2:194344/149‑1 (MQ=255)                                                                                                                         ccGCTTTG‑GCATGTGACTTCTACCATCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTAACCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTAAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCAt                           <  5:233284/148‑1 (MQ=255)                                                                                                                         ccGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCAt                           >  8:140772/1‑148 (MQ=255)                                                                                                                               tG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTc                     <  4:341641/148‑1 (MQ=255)                                                                                                                                  gCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTg                  >  5:383183/1‑149 (MQ=255)                                                                                                                                   cAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGc                 <  1:280175/149‑1 (MQ=255)                                                                                                                                    aaGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgcca                                                        >  6:109018/1‑109 (MQ=255)                                                                                                                                    aaGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTgcca                                                        <  5:109018/109‑1 (MQ=255)                                                                                                                                    aaGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCg                <  1:190533/149‑1 (MQ=255)                                                                                                                                    aaGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCg                >  6:238967/1‑149 (MQ=255)                                                                                                                                      gTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg               >  7:29009/1‑148 (MQ=255)                                                                                                                                      gTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCgg               >  3:59879/1‑148 (MQ=255)                                                                                                                                       tGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGc              >  2:174889/1‑148 (MQ=255)                                                                                                                                       tGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGc              <  1:174889/148‑1 (MQ=255)                                                                                                                                          ccagctccAGCGCCACGGTGCGCTGGAGCTGG‑CTTTCTACGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAAAGGAACCGCTACGTAGACAATGACTTCGTGCGGCAGAt          >  2:301027/7‑149 (MQ=255)                                                                                                                                          cTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATg                                                                                       <  4:361008/72‑1 (MQ=255)                                                                                                                                          cTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATg                                                                                       >  3:361008/1‑72 (MQ=255)                                                                                                                                           ttCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATg         >  7:311695/1‑149 (MQ=255)                                                                                                                                           ttCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATg         >  3:54447/1‑149 (MQ=255)                                                                                                                                            tCTACCAGCGCACCGTGGCGCTGGAGCTGGACTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATg         >  1:36577/1‑149 (MQ=255)                                                                                                                                              tACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGgc      >  1:362030/1‑149 (MQ=255)                                                                                                                                                  aGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAg  <  2:362030/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                 GAAACCGATGCCGGACAACTTCGTCCATGCCGAGTGGATTACCCTGCATGACAACCCGGCATTCCAGGCATTCGT‑CGCTTTCCTGCAGGCCGAACTGGACCGCGTCGGCCCGCAGGAGGCCGCTTTG‑GCAAGTGACTTCTACCAGCGCACCGTGGCGCTGGAGCTGG‑CTTTCTTCGATGCTGTGTACCCGGAGGGCAAATGAGCATGGATATCTTCGAGCGCCTCAAGGCCGCTGCCACGCCACAATGGAACCGCTACGTAGACCATGACTTCGTGCGGCAGATGGGCGCAG  >  NC_002947/3614574‑3614865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑