Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 234,463 G→A 6.9% D10D (GAC→GAT)  PP_0182 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947234,4630GA 6.9% 169.1 / 2.3 58D10D (GAC→GAT) PP_0182hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base A (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

ATGGGCCATCAGGTTCAGAATGTCTTCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCAGTCTATA  >  NC_002947/234316‑234599
                                                                                                                                                    |                                                                                                                                        
aTGGGCCATCAGGTTCAGAATGTCTTCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTcgtcgtcgt                                                                                                                                         >  5:179259/1‑149 (MQ=255)
 tGGGCCATCAGGTTCAGAATGTCTTCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCgtcgtcgtc                                                                                                                                        <  1:67699/149‑1 (MQ=255)
                aGAATGTCTTCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGt                                                                                                                         <  3:345469/149‑1 (MQ=255)
                       cTTCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCGTGGGTCAGCGTTGCCTCGGCGACGGCTTCGTCGTCGGAATTGt                                                                                                                                   >  8:248052/1‑132 (MQ=255)
                        ttCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGGTTCGTCGTCGTCATTGTCGATGACGGTGGgctcgc                                                                                                                 >  7:64103/1‑149 (MQ=255)
                             gCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TCTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATc                                                                                                            >  7:223446/1‑149 (MQ=255)
                                cATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATcgcg                                                                                                         >  7:68892/1‑149 (MQ=255)
                                  tAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCgg                                                                                                        >  5:281619/1‑148 (MQ=255)
                                     ccGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACt                                                                                                    >  6:108934/1‑149 (MQ=255)
                                       gACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGCGTTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTc                                                                                                   >  1:290941/1‑149 (MQ=255)
                                            gCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAgga                                                                                             <  2:290941/149‑1 (MQ=255)
                                               tCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAggatgg                                                                                          >  8:190659/1‑149 (MQ=255)
                                                  gCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAggatgggat                                                                                       <  8:68892/149‑1 (MQ=255)
                                                  gCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAggatgggat                                                                                       <  3:357425/149‑1 (MQ=255)
                                                   cTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TCTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAggatgggatg                                                                                      <  8:223446/149‑1 (MQ=255)
                                                         gAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATAGCGGTACTCCTAGGATGGGATGCAACAg                                                                                >  8:166618/1‑149 (MQ=255)
                                                           aCGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAgtg                                                                              <  7:98946/149‑1 (MQ=255)
                                                            cGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCATCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAggatg                                                                                           >  3:212620/1‑135 (MQ=255)
                                                            cGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCATCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAggatg                                                                                           <  4:212620/135‑1 (MQ=255)
                                                                     ggCCGCAGGCGGCTCGCCGC‑TTTCGTTCTGGTTTTCGATGGCTTGGGTCAGCGTGGCCTCGTCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATgg                                                                      <  7:331794/147‑1 (MQ=255)
                                                                     ggCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGc                                                                     <  4:144104/148‑1 (MQ=255)
                                                                           aGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATggcg                                                              <  8:247286/149‑1 (MQ=255)
                                                                                gcTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAAt                                                         <  2:67344/149‑1 (MQ=255)
                                                                                    gccgc‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGccc                                                     >  4:29256/1‑149 (MQ=255)
                                                                                        c‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGAGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGc                                                 <  1:117464/149‑1 (MQ=255)
                                                                                                tCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGTTGGGCTCGATCATCGCGGTACTCCTAGGATTGGATGAAACAGTGTGCCATGGATATCGCGGCAATTCCCGGGCATTGACg                                          >  8:330931/1‑149 (MQ=255)
                                                                                                    gTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCTCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGAc                                           <  7:172855/144‑1 (MQ=255)
                                                                                                    gTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGTGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACg                                          <  7:104366/145‑1 (MQ=255)
                                                                                                    gTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGa                                            <  3:29256/143‑1 (MQ=255)
                                                                                                    gTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGCCgcc                                        <  6:166435/147‑3 (MQ=255)
                                                                                                    gTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACgg                                         <  7:190659/146‑1 (MQ=255)
                                                                                                     ttttCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTg                                     >  7:30507/1‑149 (MQ=255)
                                                                                                     ttttCGATGGCTTGGGTCAGCGTTGCCTCGCCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTg                                     >  5:373114/1‑149 (MQ=255)
                                                                                                       ttCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGc                                    <  5:161403/148‑1 (MQ=255)
                                                                                                       ttCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCa                                   >  5:10045/1‑149 (MQ=255)
                                                                                                          gATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGa                                <  5:108934/149‑1 (MQ=255)
                                                                                                           aTGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGAc                               >  5:189529/1‑149 (MQ=255)
                                                                                                               cTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTg                           <  6:10045/149‑1 (MQ=255)
                                                                                                                ttGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGt                                      <  8:309872/137‑1 (MQ=255)
                                                                                                                ttGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGt                                      >  7:309872/1‑137 (MQ=255)
                                                                                                                  gggTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGAAACAGTGTTCCATGGCTATGGCGGCAATGACCGGGAATTGACGGGGTGc                                    >  8:125399/1‑137 (MQ=255)
                                                                                                                  gggTCAGCGTTGCCGCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGc                                    <  7:125399/137‑1 (MQ=255)
                                                                                                                   ggTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTc                        <  8:30507/148‑1 (MQ=255)
                                                                                                                    gTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAg                      >  5:362601/1‑149 (MQ=255)
                                                                                                                     tCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGt                     >  3:358819/1‑149 (MQ=255)
                                                                                                                     tCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTACTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCCGCAATGCCCGGGCATTGACGGTGTGCATGACGCTGGTAAGt                     >  6:291118/1‑149 (MQ=255)
                                                                                                                         cGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTgg                   >  8:145591/1‑147 (MQ=255)
                                                                                                                           ttGCCTCGGCGTAGGCTTCGTCGGCGTCATTGTCGATGTCGGAGGGCTCGCCCATCGCGGTACTCCTCGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAgg               <  8:64103/149‑1 (MQ=255)
                                                                                                                               cTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGgtgt            >  3:80281/1‑148 (MQ=255)
                                                                                                                               cTCGGCGAAGGCTTCGTCGTCATCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGgtgt            >  5:204102/1‑148 (MQ=255)
                                                                                                                                tCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGtt           >  7:268709/1‑148 (MQ=255)
                                                                                                                                 cGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCa         >  2:198372/1‑149 (MQ=255)
                                                                                                                                   gCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGt                         <  5:298226/131‑1 (MQ=255)
                                                                                                                                   gCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGt                         >  6:298226/1‑131 (MQ=255)
                                                                                                                                    cGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCAGTc      <  7:166618/149‑1 (MQ=255)
                                                                                                                                      aaGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCAGTCta    >  4:274645/1‑149 (MQ=255)
                                                                                                                                       aGGCTTCGTCGTCATCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCAGTCtat   <  6:204102/149‑1 (MQ=255)
                                                                                                                                        ggCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCAGTCtata  <  6:281619/149‑1 (MQ=255)
                                                                                                                                          cTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAgg                                                                                              <  7:304305/55‑1 (MQ=255)
                                                                                                                                          cTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAgg                                                                                              >  8:304305/1‑55 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
ATGGGCCATCAGGTTCAGAATGTCTTCACGCTCATAGCCGACCAGCGTCAGCTTGTTGAACGTGGCCTTGGCCGCAGGCGGCTCGCCGC‑TTTCGATCTGGTTTTCGATGGCTTGGGTCAGCGTTGCCTCGGCGAAGGCTTCGTCGTCGTCATTGTCGATGTCGGTGGGCTCGCTCATCGCGGTACTCCTAGGATGGGATGCAACAGTGTGCCATGGCTATGGCGGCAATGCCCGGGCATTGACGGGGTGCATGACGCTGGTCAGTGGCAGGTGTTCAGTCTATA  >  NC_002947/234316‑234599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑