Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,633,146 A→G 8.2% Q206Q (CAA→CAG)  PP_3201 → BNR domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,633,1460AG 8.2% 172.5 / 4.8 61Q206Q (CAA→CAG) PP_3201BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (25/31);  new base G (3/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

GAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTCTGCTGCTCGGC  >  NC_002947/3633002‑3633290
                                                                                                                                                |                                                                                                                                                
gAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAgg                                                                                                                                                >  1:194786/1‑147 (MQ=255)
 aaTTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                                                                                                                                               <  3:371196/147‑1 (MQ=255)
   tttCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                                                                                                                            >  6:24868/1‑148 (MQ=255)
       aCCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGgt                                                                                                                                       >  1:113320/1‑149 (MQ=255)
         cgctGCCCGAACGGGCCGGGTCGTCGGTGCCTAGGGGGGGGCAATGCCCAGCTAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg                                                                                                                                                 <  1:381347/134‑1 (MQ=255)
          gATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGGTTGCCTTGGCGCGCCAGGGCATACAGgtgca                                                                                                                                    <  4:104361/149‑3 (MQ=38)
           ctGCCCGCCACGGCCTGGTCGTCGGTGCCTACGGGGGGGCCATGCGCACCGACGACGGCGTGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTAc                                                                                                                                   <  6:77211/148‑1 (MQ=255)
               ccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCg                                                                                                                               >  3:297685/1‑149 (MQ=255)
               ccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGGTTGCCTTGGCGCGCCAGGGCATACAGgtg                                                                                                                                      >  1:8503/1‑142 (MQ=39)
               ccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGGTTGCCTTGGCGCGCCAGGGCATACAGgtg                                                                                                                                      <  2:8503/142‑1 (MQ=39)
                cGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGc                                                                                                                              >  6:40846/1‑149 (MQ=255)
                    aCGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGc                                                                                                                          <  5:24868/149‑1 (MQ=255)
                         cTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAg                                                                                                                       >  8:153774/1‑147 (MQ=255)
                         cTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAg                                                                                                                       >  3:353941/1‑147 (MQ=255)
                            gtcgtcGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAgg                                                                                                                   <  5:322281/148‑1 (MQ=255)
                             tcgtcgGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGc                                                                                                                 <  6:191098/149‑1 (MQ=255)
                             tcgtcgGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGc                                                                                                                 <  1:368240/149‑1 (MQ=255)
                               gtcgGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCct                                                                                                               >  6:62993/1‑149 (MQ=255)
                                       cTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTATATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTtc                                                                                                       <  4:210464/149‑1 (MQ=255)
                                        tACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTtcg                                                                                                      >  5:184319/1‑149 (MQ=255)
                                               tGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGt                                                                                               <  7:123129/149‑1 (MQ=255)
                                                ggCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGt                                                                                               >  2:328932/1‑148 (MQ=255)
                                                   cATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATg                                                                                           <  5:311934/149‑1 (MQ=255)
                                                   cATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATg                                                                                           <  5:40846/149‑1 (MQ=255)
                                                    aTGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATgg                                                                                          >  3:138630/1‑149 (MQ=255)
                                                       cgcACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCg                                                                                        <  4:353941/148‑1 (MQ=255)
                                                        gcACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGt                                                                                      >  7:201289/1‑149 (MQ=255)
                                                                         cgcAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCt                                                                     <  2:95017/149‑1 (MQ=255)
                                                                              cTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACg                                                                >  4:371487/1‑149 (MQ=255)
                                                                               tGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACgg                                                               >  2:250264/1‑149 (MQ=255)
                                                                                ggCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACggg                                                              >  2:169718/1‑149 (MQ=255)
                                                                                 gCAGTCGGAGCTCGGCCGACGGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGc                                                             <  5:265918/149‑1 (MQ=255)
                                                                                 gCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACggg                                                              <  8:201289/148‑1 (MQ=255)
                                                                                    gTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCt                                                          <  4:297685/149‑1 (MQ=255)
                                                                                       ggAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTAc                                                        <  5:212438/148‑1 (MQ=255)
                                                                                        gagaTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGa                                                      <  6:184319/149‑1 (MQ=255)
                                                                                         agaTGGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCGTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGAt                                                     <  6:82689/149‑1 (MQ=255)
                                                                                         agaTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGAt                                                     <  3:203129/149‑1 (MQ=255)
                                                                                         agaTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGAt                                                     <  3:371487/149‑1 (MQ=255)
                                                                                           aTCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATgg                                                   >  7:270368/1‑149 (MQ=255)
                                                                                              ggCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCa                                                 <  3:22108/148‑1 (MQ=255)
                                                                                                ccGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGc                                               >  4:94825/1‑148 (MQ=255)
                                                                                                          aaCGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTcgccgc                                    >  3:256030/1‑149 (MQ=255)
                                                                                                                    gACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGtt                           <  1:250264/148‑1 (MQ=255)
                                                                                                                    gACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGtt                           <  3:94825/148‑1 (MQ=255)
                                                                                                                     aCTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGc                         >  6:57189/1‑149 (MQ=255)
                                                                                                                      cTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGcc                        <  2:113320/149‑1 (MQ=255)
                                                                                                                       tGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGccc                       >  2:218380/1‑149 (MQ=255)
                                                                                                                           ccTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATg                   <  7:153774/149‑1 (MQ=255)
                                                                                                                            ctggttGCCTTGGCGCGCCAGGGCATACAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATgg                  >  1:229005/6‑149 (MQ=255)
                                                                                                                            ctggttGCCTTGGCGCGCCAGGGCATACAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCAGATgg                  >  6:211853/6‑149 (MQ=255)
                                                                                                                               tATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCg               <  4:138630/149‑1 (MQ=255)
                                                                                                                                 tGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTc             <  1:218380/149‑1 (MQ=255)
                                                                                                                                 tGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTc             >  2:266212/1‑149 (MQ=255)
                                                                                                                                    ccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCAt                                                                                >  2:54251/1‑79 (MQ=255)
                                                                                                                                    ccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCAt                                                                                <  1:54251/79‑1 (MQ=255)
                                                                                                                                    ccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCACTGCACGGGCCCTACGATGGCAGCTACTTcgccg                                     <  7:226955/122‑1 (MQ=255)
                                                                                                                                    ccTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCACTGCACGGGCCCTACGATGGCAGCTACTTcgccg                                     >  8:226955/1‑122 (MQ=255)
                                                                                                                                        gcgcgcCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTctgctg        <  5:62993/147‑1 (MQ=255)
                                                                                                                                        gcgcgcCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTCTgctgct      >  7:72058/1‑149 (MQ=255)
                                                                                                                                            gcCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTCTGCTGCTcggc  >  1:83632/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGCTGCTTCGCTCGCGTGATGGCGGTGAGCATTACGAGGCGCTGCACGGGCCCTACGATGGCAGCTACTTCGCCGCCACCGTGTTGCCCGATGGCCGTCTGCTGCTCGGC  >  NC_002947/3633002‑3633290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑