Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,686,436	C→T	8.4%	L282L (CTG→TTG)	PP_3251 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,686,436	0	C	T	8.4%	205.3 / 5.7	71	L282L (CTG→TTG)	PP_3251	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/34);  new base T (4/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTC  >  NC_002947/3686288‑3686584                                                                                                                                                     |                                                                                                                                                     cgccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGAcc                                                                                                                                                      <  3:56108/149‑1 (MQ=255)  gccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCt                                                                                                                                                     <  8:307749/149‑1 (MQ=255)  gccAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCACCTGAATGACCGGGGTATCACCACGCCAGACCTCATCCGCCAGTTCGACCt                                                                                                                                                     <  2:376546/149‑1 (MQ=255)      gCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                                 <  3:138064/149‑1 (MQ=255)           cACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCAGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCg                                                                                                                                             >  6:274203/1‑148 (MQ=255)            aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                           <  7:322526/149‑1 (MQ=255)              gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAAc                                                                                                                                          <  8:310102/148‑1 (MQ=255)              gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAAc                                                                                                                                          >  7:310102/1‑148 (MQ=255)              gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAgagatccg                                                                                                                                         >  3:291170/1‑142 (MQ=255)              gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACt                                                                                                                                         >  7:18281/1‑149 (MQ=255)              gcccgccTGCCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                                 <  4:291170/141‑1 (MQ=255)                    cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                           <  5:281588/141‑1 (MQ=255)                    cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                           >  6:281588/1‑141 (MQ=255)                           ttCATGAGCTGAACCAGCTAAGCCCCTCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                            <  8:202979/149‑1 (MQ=255)                           ttCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                            <  8:197641/149‑1 (MQ=255)                           ttCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                            <  7:258769/149‑1 (MQ=255)                               ggCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                        <  6:311602/149‑1 (MQ=255)                               ggCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                        <  8:149066/149‑1 (MQ=255)                                gcgcTGAACCAGCTAAGCCCCGCGCTTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGTCCGGGGTATCGCCACGCGAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTg                                                                                                                                    <  5:30090/136‑1 (MQ=255)                                gcgcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACGCTTTg                                                                                                                                    >  6:30090/1‑136 (MQ=255)                                     gAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGc                                                                                                                  >  3:157455/1‑149 (MQ=255)                                      aaCCAGCTAAGCCCCGCGCATGATCCGCTGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCg                                                                                                                 >  1:222275/1‑149 (MQ=255)                                      aaCCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAg                                                                                                                   <  8:18281/147‑1 (MQ=255)                                                gCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGc                                                                                                       <  7:100663/149‑1 (MQ=255)                                                gCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGc                                                                                                       >  6:364803/1‑149 (MQ=255)                                                 ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGACCGCAAGCgg                                                                                                                <  3:227569/139‑1 (MQ=255)                                                 ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                <  3:232358/139‑1 (MQ=255)                                                 ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                >  4:232358/1‑139 (MQ=255)                                                 ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                >  4:227569/1‑139 (MQ=255)                                                        cATGATCTGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCTCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                               <  5:274203/149‑1 (MQ=255)                                                        cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                               <  2:371623/149‑1 (MQ=255)                                                        cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                               <  8:32021/149‑1 (MQ=255)                                                         aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatg                                                                                              <  4:157455/149‑1 (MQ=255)                                                         aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                               >  3:238583/1‑148 (MQ=255)                                                                gCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGcc                                                                                        >  4:138644/1‑148 (MQ=255)                                                                 cAGGCGGTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGa                                                                                      <  2:140644/149‑1 (MQ=255)                                                                    gCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCa                                                                                   <  3:138644/149‑1 (MQ=255)                                                                      gTTCTACGCCAGGGCTCGCTTAGGCTACCGCCTGAATGAGCGGGGTATCGCCACGCCAGACCTGATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                 <  6:202932/149‑1 (MQ=255)                                                                      gTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                 >  6:156950/1‑149 (MQ=255)                                                                           aCGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                            >  4:126360/1‑149 (MQ=255)                                                                            cGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgcc                                                                           <  1:262582/149‑1 (MQ=255)                                                                               cAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTg                                                                         >  3:101139/1‑148 (MQ=255)                                                                                     gcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                  >  4:194153/1‑149 (MQ=255)                                                                                        cTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCa                                                               <  5:364803/149‑1 (MQ=255)                                                                                           aGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                            >  8:25797/1‑149 (MQ=255)                                                                                           aGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                            >  2:102534/1‑149 (MQ=255)                                                                                                    ccTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                   >  2:368870/1‑149 (MQ=255)                                                                                                          tGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggctgg                                             <  4:238583/149‑1 (MQ=255)                                                                                                             ccGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCTGCCTGCAGCAGCGCACGATCAATCAATGGCTGGcc                                           >  1:19672/1‑148 (MQ=255)                                                                                                             ccGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCg                                          >  6:60993/1‑149 (MQ=255)                                                                                                                 ggTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAAcc                                      <  5:60993/149‑1 (MQ=255)                                                                                                                    aTCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGCTGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCgg                                   >  5:199484/1‑149 (MQ=255)                                                                                                                     tCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCgg                                   <  5:156950/148‑1 (MQ=255)                                                                                                                     tCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgc                                  <  7:25797/149‑1 (MQ=255)                                                                                                                      cgccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgcg                                 >  5:153650/1‑149 (MQ=255)                                                                                                                        ccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGACCCCGgcgc                                >  6:222768/1‑148 (MQ=255)                                                                                                                         cacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCa                              >  7:292660/1‑149 (MQ=255)                                                                                                                           cgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATg                            <  7:102816/149‑1 (MQ=255)                                                                                                                            gccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                           <  8:292660/149‑1 (MQ=255)                                                                                                                              caGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGGGCCATGc                           >  2:153738/1‑147 (MQ=255)                                                                                                                                      tgcaaacAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGt                 >  1:103697/7‑149 (MQ=255)                                                                                                                                      aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTAGATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGt                 >  1:105993/1‑149 (MQ=255)                                                                                                                                        ccGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                >  4:341944/1‑52 (MQ=255)                                                                                                                                        ccGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                <  3:341944/52‑1 (MQ=255)                                                                                                                                           ccAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg            >  1:199974/1‑149 (MQ=255)                                                                                                                                            cAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg            >  2:315203/1‑148 (MQ=255)                                                                                                                                               ttCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCAtt                    <  5:377813/137‑1 (MQ=255)                                                                                                                                               ttCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCAtt                    >  6:377813/1‑137 (MQ=255)                                                                                                                                                 cGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTa       <  6:153650/148‑1 (MQ=255)                                                                                                                                                  gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTa       >  3:271833/1‑147 (MQ=255)                                                                                                                                                  gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACa     <  2:222275/149‑1 (MQ=255)                                                                                                                                                   aCCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAg    >  1:318894/1‑149 (MQ=255)                                                                                                                                                     cTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGtc  <  3:104920/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CGCCAGCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTC  >  NC_002947/3686288‑3686584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑