Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,686,441	A→G	8.4%	Q283Q (CAA→CAG)	PP_3251 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,686,441	0	A	G	8.4%	198.5 / 6.8	71	Q283Q (CAA→CAG)	PP_3251	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/33);  new base G (4/2);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCATG  >  NC_002947/3686293‑3686589                                                                                                                                                     |                                                                                                                                                     gCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                                      <  3:138064/149‑1 (MQ=255)      cACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCAGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCg                                                                                                                                                  >  6:274203/1‑148 (MQ=255)       aCGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                <  7:322526/149‑1 (MQ=255)         gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAAc                                                                                                                                               <  8:310102/148‑1 (MQ=255)         gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGCCTTGCAGGTCGAAc                                                                                                                                               >  7:310102/1‑148 (MQ=255)         gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAgagatccg                                                                                                                                              >  3:291170/1‑142 (MQ=255)         gcccgccTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACt                                                                                                                                              >  7:18281/1‑149 (MQ=255)         gcccgccTGCCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCaa                                                                                                                                                      <  4:291170/141‑1 (MQ=255)               cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                >  6:281588/1‑141 (MQ=255)               cTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGaa                                                                                                                                                <  5:281588/141‑1 (MQ=255)                      ttCATGAGCTGAACCAGCTAAGCCCCTCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                                 <  8:202979/149‑1 (MQ=255)                      ttCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                                 <  7:258769/149‑1 (MQ=255)                      ttCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCtt                                                                                                                                 <  8:197641/149‑1 (MQ=255)                          ggCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                             <  8:149066/149‑1 (MQ=255)                          ggCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAAc                                                                                                                             <  6:311602/149‑1 (MQ=255)                           gcgcTGAACCAGCTAAGCCCCGCGCTTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGTCCGGGGTATCGCCACGCGAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTg                                                                                                                                         <  5:30090/136‑1 (MQ=255)                           gcgcTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACGCTTTg                                                                                                                                         >  6:30090/1‑136 (MQ=255)                                gAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGc                                                                                                                       >  3:157455/1‑149 (MQ=255)                                 aaCCAGCTAAGCCCCGCGCATGATCCGCTGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCg                                                                                                                      >  1:222275/1‑149 (MQ=255)                                 aaCCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAg                                                                                                                        <  8:18281/147‑1 (MQ=255)                                           gCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGc                                                                                                            >  6:364803/1‑149 (MQ=255)                                           gCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGc                                                                                                            <  7:100663/149‑1 (MQ=255)                                            ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGACCGCAAGCgg                                                                                                                     <  3:227569/139‑1 (MQ=255)                                            ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                     >  4:227569/1‑139 (MQ=255)                                            ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                     >  4:232358/1‑139 (MQ=255)                                            ccccGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                     <  3:232358/139‑1 (MQ=255)                                                   cATGATCTGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCTCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                                    <  5:274203/149‑1 (MQ=255)                                                   cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                                    <  8:32021/149‑1 (MQ=255)                                                   cATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                                    <  2:371623/149‑1 (MQ=255)                                                    aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGatg                                                                                                   <  4:157455/149‑1 (MQ=255)                                                    aTGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGat                                                                                                    >  3:238583/1‑148 (MQ=255)                                                           gCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGcc                                                                                             >  4:138644/1‑148 (MQ=255)                                                            cAGGCGGTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGa                                                                                           <  2:140644/149‑1 (MQ=255)                                                               gCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCa                                                                                        <  3:138644/149‑1 (MQ=255)                                                                 gTTCTACGCCAGGGCTCGCTTAGGCTACCGCCTGAATGAGCGGGGTATCGCCACGCCAGACCTGATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                      <  6:202932/149‑1 (MQ=255)                                                                 gTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCAtt                                                                                      >  6:156950/1‑149 (MQ=255)                                                                      aCGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgc                                                                                 >  4:126360/1‑149 (MQ=255)                                                                       cGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTgccgcc                                                                                <  1:262582/149‑1 (MQ=255)                                                                          cAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTg                                                                              >  3:101139/1‑148 (MQ=255)                                                                                gcgcTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCagcagc                                                                       >  4:194153/1‑149 (MQ=255)                                                                                   cTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCa                                                                    <  5:364803/149‑1 (MQ=255)                                                                                      aGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                                 >  8:25797/1‑149 (MQ=255)                                                                                      aGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGa                                                                 >  2:102534/1‑149 (MQ=255)                                                                                               ccTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATcaatcaat                                                        >  2:368870/1‑149 (MQ=255)                                                                                                     tGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAAtggctgg                                                  <  4:238583/149‑1 (MQ=255)                                                                                                        ccGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCTGCCTGCAGCAGCGCACGATCAATCAATGGCTGGcc                                                >  1:19672/1‑148 (MQ=255)                                                                                                        ccGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCg                                               >  6:60993/1‑149 (MQ=255)                                                                                                            ggTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAAcc                                           <  5:60993/149‑1 (MQ=255)                                                                                                               aTCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGCTGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCgg                                        >  5:199484/1‑149 (MQ=255)                                                                                                                tCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCgg                                        <  5:156950/148‑1 (MQ=255)                                                                                                                tCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgc                                       <  7:25797/149‑1 (MQ=255)                                                                                                                 cgccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGgcg                                      >  5:153650/1‑149 (MQ=255)                                                                                                                   ccacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGACCCCGgcgc                                     >  6:222768/1‑148 (MQ=255)                                                                                                                    cacgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCa                                   >  7:292660/1‑149 (MQ=255)                                                                                                                      cgccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATg                                 <  7:102816/149‑1 (MQ=255)                                                                                                                       gccaGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGc                                <  8:292660/149‑1 (MQ=255)                                                                                                                         caGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGGGCCATGc                                >  2:153738/1‑147 (MQ=255)                                                                                                                                 tgcaaacAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGt                      >  1:103697/7‑149 (MQ=255)                                                                                                                                 aTCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTAGATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGt                      >  1:105993/1‑149 (MQ=255)                                                                                                                                   ccGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                     >  4:341944/1‑52 (MQ=255)                                                                                                                                   ccGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCgg                                                                                                                     <  3:341944/52‑1 (MQ=255)                                                                                                                                      ccAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg                 >  1:199974/1‑149 (MQ=255)                                                                                                                                       cAGTTCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCg                 >  2:315203/1‑148 (MQ=255)                                                                                                                                          ttCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCAtt                         >  6:377813/1‑137 (MQ=255)                                                                                                                                          ttCGCCTTGCAGGTCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCAtt                         <  5:377813/137‑1 (MQ=255)                                                                                                                                            cGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTa            <  6:153650/148‑1 (MQ=255)                                                                                                                                             gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTa            >  3:271833/1‑147 (MQ=255)                                                                                                                                             gACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACa          <  2:222275/149‑1 (MQ=255)                                                                                                                                              aCCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAg         >  1:318894/1‑149 (MQ=255)                                                                                                                                                cTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGtc       <  3:104920/149‑1 (MQ=255)                                                                                                                                                  gCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCTATGGCTGGCCGAACCCGGCGCCATGccc                              <  1:219249/124‑1 (MQ=255)                                                                                                                                                  gCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGccc                              >  2:219249/1‑124 (MQ=255)                                                                                                                                                   cAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCTATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGtctc     <  1:102534/148‑1 (MQ=255)                                                                                                                                                     aGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCATg  <  2:337280/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GCGGCCACGCCCGCCTGGCAATTCAGGCGCTGAACCAGCTAAGCCCCGCGCATGATCCGCAGGCGTTCTACGCCAGGGCGCGCTTAGGCTACCGCCTGAATGACCGGGGTATCGCCACGCCAGACCTCATCCGCCAGTTCGACCTGCAAGGCGAACTGTTTGCAGCCCTTGAACGCAAGCGGGTCTATGGCCAATTGATGCATGCCGACCATTGCCGCCTGCAGCAGCGCACGATCAATCAATGGCTGGCCGAACCCGGCGCCATGCCCGCATTCGTTGCCGCATTACAGTCTCATG  >  NC_002947/3686293‑3686589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑