Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,717,846 C→A 6.9% V249V (GTG→GTT)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,717,8460CA 6.9% 176.3 / 3.9 58V249V (GTG→GTT) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base C (30/24);  new base A (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

CGGGGTGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGT  >  NC_002947/3717702‑3717991
                                                                                                                                                 |                                                                                                                                                  
cGGGGTGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGc                                                                                                                                                >  2:272637/1‑149 (MQ=255)
     tGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTGAACGCCTTca                                                                                                                                           <  5:338381/149‑2 (MQ=255)
     tGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTGAACGCCTTca                                                                                                                                           <  2:77915/149‑2 (MQ=255)
      ggccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTcgc                                                                                                                                          >  4:278508/1‑149 (MQ=255)
        ccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCgg                                                                                                                                        <  1:175550/149‑1 (MQ=255)
         cggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCAGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCgg                                                                                                                                        >  1:269393/1‑149 (MQ=255)
                       aCCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTgcgc                                                                                                                        <  3:38771/149‑1 (MQ=255)
                        ccTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTgcgc                                                                                                                        <  7:310949/148‑1 (MQ=255)
                        ccTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTgcgc                                                                                                                        >  8:310949/1‑148 (MQ=255)
                                   ccGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGc                                                                                                                             >  4:64539/1‑132 (MQ=255)
                                   ccGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGc                                                                                                                             <  3:64539/132‑1 (MQ=255)
                                    gggCGCCGTTGGCGCC‑GGCACCGATCACGGCCACCTGCACATTGCTTGCGAGTGGGGCCATTTTGAGCGCCCCTTGATGGCTGGCGTGCGTTTGTTCATGAAGGCGTTc                                                                                                                                                    <  6:381310/108‑1 (MQ=255)
                                    cggcggCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCt                                                                                                           <  4:248792/149‑1 (MQ=255)
                                    cGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCt                                                                                                           >  2:1430/1‑149 (MQ=255)
                                    cGGCCCCCATGGCCCC‑GGCACCGATCACAGCCACCTGCACAATGCATGAGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTc                                                                                                                                                    >  5:381310/1‑109 (MQ=255)
                                     ggCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTg                                                                                                          <  3:39762/149‑1 (MQ=255)
                                       cgcCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCa                                                                                                        <  8:170929/149‑1 (MQ=255)
                                       cgcCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCa                                                                                                        <  2:23367/149‑1 (MQ=255)
                                          ccATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGt                                                                                                     >  3:368789/1‑149 (MQ=255)
                                          ccATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGt                                                                                                     >  4:35656/1‑149 (MQ=255)
                                          ccATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGACCCTTGAAGGCTGGCGTGCGTTTGGTCATGAAGGCGGGCCCGCAGTCGCGGTCGTC‑GGCGCGGAGCCCGGCCCGGCGCGGCAggg                                                                                                     >  4:304185/1‑148 (MQ=255)
                                            aTGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTcg                                                                                                   >  2:195016/1‑149 (MQ=255)
                                               gcgcC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCgcgc                                                                                                <  3:35656/149‑1 (MQ=255)
                                                 gcC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCgctc                                                                                              <  3:278508/149‑1 (MQ=255)
                                                        aCCGATCACCGCCACCTGCAAATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTA‑ATCGCTGCGCCCTGCCAGGAGATGCAGGTCGCgctccatctc                                                                                        >  6:227024/1‑149 (MQ=255)
                                                               aCCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCTGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTgc                                                                                 >  8:193486/1‑149 (MQ=255)
                                                                    cACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTc                                                                            <  6:255585/149‑1 (MQ=255)
                                                                             aTTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCAtt                                                                   >  8:142765/1‑149 (MQ=255)
                                                                                 cttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAg                                                                                                       <  6:176181/109‑1 (MQ=255)
                                                                                 cttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAg                                                                                                       >  5:176181/1‑109 (MQ=255)
                                                                                 cttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTc                                                                >  5:240577/1‑148 (MQ=255)
                                                                                  ttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCg                                                               >  6:33967/1‑148 (MQ=255)
                                                                                            ggCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCAtt                                                    >  5:196912/1‑149 (MQ=255)
                                                                                            ggCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCAtt                                                    <  7:193486/149‑1 (MQ=255)
                                                                                               cATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAg                                                 <  1:195016/149‑1 (MQ=255)
                                                                                                aTTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAgg                                                >  5:338329/1‑149 (MQ=255)
                                                                                                  tttCAGCGCCCCTTGAAGGCTGGCGTGCGTGTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGGC‑CTCGCTGCGCCCTGCCAGGCGCTGCATGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAgg                                                <  8:319413/147‑1 (MQ=255)
                                                                                                   ttCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCt                                              <  7:99831/148‑1 (MQ=255)
                                                                                                       gcgcCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCt                                         >  5:75859/1‑149 (MQ=255)
                                                                                                            ccTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGt                                                                 >  7:347904/1‑120 (MQ=255)
                                                                                                            ccTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGt                                                                 <  8:347904/120‑1 (MQ=255)
                                                                                                                gAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAgt                                 <  1:272637/148‑1 (MQ=255)
                                                                                                                   ggCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTgcgc                                          <  1:288854/136‑1 (MQ=255)
                                                                                                                   ggCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTgcgc                                          >  2:288854/1‑136 (MQ=255)
                                                                                                                   ggCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAgtgt                               >  1:4768/1‑147 (MQ=255)
                                                                                                                        gcgtgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCTCTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGcc                         >  1:74550/1‑149 (MQ=255)
                                                                                                                          gtgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTa                      <  3:53140/149‑1 (MQ=255)
                                                                                                                           tgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAg                     <  6:75859/149‑1 (MQ=255)
                                                                                                                            gcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAgg                           >  4:72993/1‑142 (MQ=255)
                                                                                                                            gcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAgg                           <  3:72993/142‑1 (MQ=255)
                                                                                                                             cgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGt                   <  4:368789/149‑1 (MQ=255)
                                                                                                                                   ttCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAgt                                 >  7:306187/1‑129 (MQ=255)
                                                                                                                                   ttCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAgt                                 <  8:306187/129‑1 (MQ=255)
                                                                                                                                    tCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGtgggctgg            >  3:90719/1‑149 (MQ=255)
                                                                                                                                      aTGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGtgggctgggc          >  6:112123/1‑149 (MQ=255)
                                                                                                                                       cgAAGGCGTGAACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGggcg         >  8:236908/2‑149 (MQ=255)
                                                                                                                                       cgAAGGCGTGAACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGggcg         >  3:154344/2‑149 (MQ=255)
                                                                                                                                        gAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGAGGGCTGggcgg        >  6:26558/1‑149 (MQ=255)
                                                                                                                                          aGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGggcggc       >  8:84498/1‑148 (MQ=255)
                                                                                                                                              gTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGt  >  2:94870/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGGGGTGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCC‑GGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTC‑CTCGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGT  >  NC_002947/3717702‑3717991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑