Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	255,258	A→G	5.1%	intergenic (‑142/+21)	PP_0203 ← / ← PP_t74	dipeptidase/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	255,258	0	A	G	5.1%	236.0 / 2.4	78	intergenic (‑142/+21)	PP_0203/PP_t74	dipeptidase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (35/39);  new base G (2/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

ACTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGA  >  NC_002947/255110‑255404                                                                                                                                                     |                                                                                                                                                   aCTGCATGGTTGTGCGCATTCCTTTTCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCa                                                                                                                                                    <  4:255879/149‑1 (MQ=255) aCTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACTGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCa                                                                                                                                                    <  1:223835/149‑1 (MQ=255) aCTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCa                                                                                                                                                    <  2:95759/149‑1 (MQ=255)  cTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAg                                                                                                                                                   >  5:360844/1‑149 (MQ=255)      aTGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGcc                                                                                                                                                >  6:35584/1‑148 (MQ=255)             tgCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCtt                                                                                                                                        <  3:4475/149‑1 (MQ=255)                gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTTGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAt                                                                                                                                     >  5:72323/1‑149 (MQ=255)                gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAt                                                                                                                                     >  3:93246/1‑149 (MQ=255)                 cATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGATAAAGCCCTGGGCCTGAGCCCGGGGCtttttc                                                                                                                                    <  7:176394/149‑4 (MQ=255)                 cATTCCTTTGCCAATCATTGTTCAGGGTTACACGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                    <  6:120922/149‑1 (MQ=255)                 cATTACTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACTCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                    <  5:81672/149‑1 (MQ=255)                  aTTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                    >  3:104570/1‑148 (MQ=255)                  aTTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTg                                                                                                                                   <  2:317854/149‑1 (MQ=255)                     ccTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttcgtt                                                                                                                                 <  5:53907/148‑7 (MQ=255)                       tttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCAAGGGCTTTATTGATATg                                                                                                                              >  7:143106/1‑149 (MQ=255)                        ttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATgg                                                                                                                             >  7:6056/1‑149 (MQ=255)                            cAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACCGGACTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGgcgc                                                                                                                          >  5:291003/1‑148 (MQ=255)                              aTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACt                                                                                                                       >  1:326922/1‑149 (MQ=255)                                 aTTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcgg                                                                                                                    >  5:312931/1‑149 (MQ=255)                                    gTTCAGGGTTACCCGTTCGGTAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcg                                                                                                                  <  4:104570/148‑1 (MQ=255)                                        aGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGAt                                                                                                              <  8:6056/148‑1 (MQ=255)                                         gggTTACCCGTTCGGGAAACGGTAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTc                                                                                                            <  5:35584/149‑1 (MQ=255)                                              aCCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAc                                                                                                        <  8:293250/148‑1 (MQ=255)                                                ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTTGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                     <  6:72323/149‑1 (MQ=255)                                                ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                     >  5:2423/1‑149 (MQ=255)                                                 cGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                    <  6:295694/149‑1 (MQ=255)                                                  gTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                    >  4:118380/1‑148 (MQ=255)                                                     cGGGAAACGGGAAGACGCCCACGCAAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAc                                                                                                 <  8:165107/148‑1 (MQ=255)                                                      gggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAccc                                                                                               >  7:131720/1‑149 (MQ=255)                                                       ggAAACGTGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAcccc                                                                                              <  5:201568/149‑1 (MQ=255)                                                       ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAcccc                                                                                              >  8:96550/1‑149 (MQ=255)                                                         aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                            >  1:23664/1‑149 (MQ=255)                                                         aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                            >  6:85787/1‑149 (MQ=255)                                                         aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                            >  2:343637/1‑149 (MQ=255)                                                           aCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGCACCAGAAAGGTGACATGTCGCACAAACGAAAACGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGcc                                                                                          <  4:306921/149‑1 (MQ=255)                                                              ggAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTc                                                                                       <  2:326922/149‑1 (MQ=255)                                                                   aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCCGCCTTCGGAgg                                                                                  <  4:322589/149‑1 (MQ=255)                                                                       ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTAGCACAAACGAAAAAGCCCCGGGCTCAGCCCCAGGCCGTTATTGATATGGCGCCCTCGGCTGCATTCGAACCAACGAACCCAGCCATCCGAgcg                                                                                 >  7:270194/1‑144 (MQ=255)                                                                        cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGa                                                                                                          >  4:348044/1‑120 (MQ=255)                                                                        cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGa                                                                                                          <  3:348044/120‑1 (MQ=255)                                                                          cGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTAc                                                                           >  8:183317/1‑149 (MQ=255)                                                                             tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTActc                                                                         >  1:333576/1‑148 (MQ=255)                                                                               acacCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTAt                                                                      <  6:360844/149‑1 (MQ=255)                                                                                 acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAccc                                                                                                      <  8:248037/115‑1 (MQ=255)                                                                                 acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATc                                                                     >  3:198921/1‑148 (MQ=255)                                                                                 acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGCGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAccc                                                                                                      >  7:248037/1‑115 (MQ=255)                                                                                     aTGACCCGTGGTACGGGAGTACCAGAAAGGTTACTTGTCGCACATACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                <  3:372740/149‑1 (MQ=255)                                                                                     aTGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                >  4:27763/1‑149 (MQ=255)                                                                                        aCCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTc                                                                                                                      >  5:383716/1‑92 (MQ=255)                                                                                        aCCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTc                                                                                                                      <  6:383716/92‑1 (MQ=255)                                                                                        aCCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAg                                                             >  1:189327/1‑149 (MQ=255)                                                                                          ccGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCt                                                           <  2:23664/149‑1 (MQ=255)                                                                                            gTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCCCGACCCCTGCCTGCGGAGGGCAGTACTCTATCCAGCTGAGCtcc                                                         >  6:259428/1‑147 (MQ=255)                                                                                             tGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACg                                                        <  7:96550/149‑1 (MQ=255)                                                                                                  cGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGc                                                   <  4:93246/149‑1 (MQ=255)                                                                                                   gggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAgg                                                                                                                                                  >  3:374885/1‑53 (MQ=255)                                                                                                   gggAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAgg                                                                                                                                                  <  4:374885/53‑1 (MQ=255)                                                                                                       gTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAcgc                                              <  2:333576/149‑1 (MQ=255)                                                                                                          ccAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATACGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgca                                           <  5:202672/149‑1 (MQ=255)                                                                                                              aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgca                                       >  5:218416/1‑149 (MQ=255)                                                                                                              aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTATATCCAGCTGAGCTACGAGTGCAACGcgcacgca                                       >  7:310106/1‑149 (MQ=255)                                                                                                                aGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATg                                     <  6:218416/149‑1 (MQ=255)                                                                                                                 ggTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTTATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGa                                    <  6:291003/149‑1 (MQ=255)                                                                                                                   tGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATa                                  <  6:2423/149‑1 (MQ=255)                                                                                                                        tGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCAt                             <  3:118380/149‑1 (MQ=255)                                                                                                                               caAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTc                      <  2:189327/149‑1 (MQ=255)                                                                                                                                aaaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcac                                          <  7:78848/128‑1 (MQ=255)                                                                                                                                aaaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcac                                          >  8:78848/1‑128 (MQ=255)                                                                                                                                 aaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGt                    >  2:86322/1‑149 (MQ=255)                                                                                                                                 aaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGt                    <  1:154783/149‑1 (MQ=255)                                                                                                                                  aCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGtt                   <  7:183317/149‑1 (MQ=255)                                                                                                                                  aCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGtt                   <  8:143106/149‑1 (MQ=255)                                                                                                                                     ataaaGCCCTGGGCCTGAGCCCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                >  6:173524/3‑149 (MQ=39)                                                                                                                                     ataaaGCCCTGGGCCTGAGCCCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                >  8:38271/3‑149 (MQ=39)                                                                                                                                     aaaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACGCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                <  6:389948/149‑1 (MQ=255)                                                                                                                                      aaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACg               <  8:131720/149‑1 (MQ=255)                                                                                                                                       aaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt              <  1:343637/149‑1 (MQ=255)                                                                                                                                               gggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGcc      <  4:198921/149‑1 (MQ=255)                                                                                                                                                ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACGCATGTGTGTCGTTGACGTCGATGGCCt     >  4:23049/1‑149 (MQ=255)                                                                                                                                                ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCt     >  4:28088/1‑149 (MQ=255)                                                                                                                                                   tCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGCCCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGa  >  2:175561/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACTGCATGGTTGTGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGA  >  NC_002947/255110‑255404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑