Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 255,264 A→G 5.2% intergenic (‑148/+15) PP_0203 ← / ← PP_t74 dipeptidase/tRNA‑Arg

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947255,2640AG 5.2% 236.6 / 2.4 77intergenic (‑148/+15)PP_0203/PP_t74dipeptidase/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base A (33/40);  new base G (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

TGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCTGTG  >  NC_002947/255122‑255410
                                                                                                                                              |                                                                                                                                                  
tgCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCtt                                                                                                                                              <  3:4475/149‑1 (MQ=255)
   gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTTGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAt                                                                                                                                           >  5:72323/1‑149 (MQ=255)
   gcATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAt                                                                                                                                           >  3:93246/1‑149 (MQ=255)
    cATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGATAAAGCCCTGGGCCTGAGCCCGGGGCtttttc                                                                                                                                          <  7:176394/149‑4 (MQ=255)
    cATTCCTTTGCCAATCATTGTTCAGGGTTACACGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                          <  6:120922/149‑1 (MQ=255)
    cATTACTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACTCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                          <  5:81672/149‑1 (MQ=255)
     aTTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTAtt                                                                                                                                          >  3:104570/1‑148 (MQ=255)
     aTTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTg                                                                                                                                         <  2:317854/149‑1 (MQ=255)
        ccTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCTGGGCCTGAGCCCGGGGCtttttcgtt                                                                                                                                       <  5:53907/148‑7 (MQ=255)
          tttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCAAGGGCTTTATTGATATg                                                                                                                                    >  7:143106/1‑149 (MQ=255)
           ttGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATgg                                                                                                                                   >  7:6056/1‑149 (MQ=255)
               cAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACCGGACTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGgcgc                                                                                                                                >  5:291003/1‑148 (MQ=255)
                 aTCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACt                                                                                                                             >  1:326922/1‑149 (MQ=255)
                    aTTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcgg                                                                                                                          >  5:312931/1‑149 (MQ=255)
                       gTTCAGGGTTACCCGTTCGGTAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTcggcg                                                                                                                        <  4:104570/148‑1 (MQ=255)
                           aGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGAt                                                                                                                    <  8:6056/148‑1 (MQ=255)
                            gggTTACCCGTTCGGGAAACGGTAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTc                                                                                                                  <  5:35584/149‑1 (MQ=255)
                                 aCCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAc                                                                                                              <  8:293250/148‑1 (MQ=255)
                                   ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTTGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                           <  6:72323/149‑1 (MQ=255)
                                   ccGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCa                                                                                                           >  5:2423/1‑149 (MQ=255)
                                    cGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                          <  6:295694/149‑1 (MQ=255)
                                     gTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCAc                                                                                                          >  4:118380/1‑148 (MQ=255)
                                        cGGGAAACGGGAAGACGCCCACGCAAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAc                                                                                                       <  8:165107/148‑1 (MQ=255)
                                         gggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAccc                                                                                                     >  7:131720/1‑149 (MQ=255)
                                          ggAAACGTGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAcccc                                                                                                    <  5:201568/149‑1 (MQ=255)
                                          ggAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGAcccc                                                                                                    >  8:96550/1‑149 (MQ=255)
                                            aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                                  >  1:23664/1‑149 (MQ=255)
                                            aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                                  >  2:343637/1‑149 (MQ=255)
                                            aaaCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTg                                                                                                  >  6:85787/1‑149 (MQ=255)
                                              aCGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGCACCAGAAAGGTGACATGTCGCACAAACGAAAACGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGcc                                                                                                <  4:306921/149‑1 (MQ=255)
                                                 ggAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTc                                                                                             <  2:326922/149‑1 (MQ=255)
                                                      aCGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCCGCCTTCGGAgg                                                                                        <  4:322589/149‑1 (MQ=255)
                                                          ccACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTAGCACAAACGAAAAAGCCCCGGGCTCAGCCCCAGGCCGTTATTGATATGGCGCCCTCGGCTGCATTCGAACCAACGAACCCAGCCATCCGAgcg                                                                                       >  7:270194/1‑144 (MQ=255)
                                                           cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGa                                                                                                                >  4:348044/1‑120 (MQ=255)
                                                           cACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGa                                                                                                                <  3:348044/120‑1 (MQ=255)
                                                             cGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTAc                                                                                 >  8:183317/1‑149 (MQ=255)
                                                                tAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTActc                                                                               >  1:333576/1‑148 (MQ=255)
                                                                  acacCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTAt                                                                            <  6:360844/149‑1 (MQ=255)
                                                                    acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAccc                                                                                                            <  8:248037/115‑1 (MQ=255)
                                                                    acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATc                                                                           >  3:198921/1‑148 (MQ=255)
                                                                    acCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGCGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAAccc                                                                                                            >  7:248037/1‑115 (MQ=255)
                                                                        aTGACCCGTGGTACGGGAGTACCAGAAAGGTTACTTGTCGCACATACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                      <  3:372740/149‑1 (MQ=255)
                                                                        aTGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCt                                                                      >  4:27763/1‑149 (MQ=255)
                                                                           aCCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTc                                                                                                                            >  5:383716/1‑92 (MQ=255)
                                                                           aCCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTc                                                                                                                            <  6:383716/92‑1 (MQ=255)
                                                                           aCCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAg                                                                   >  1:189327/1‑149 (MQ=255)
                                                                             ccGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCt                                                                 <  2:23664/149‑1 (MQ=255)
                                                                               gTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCCCGACCCCTGCCTGCGGAGGGCAGTACTCTATCCAGCTGAGCtcc                                                               >  6:259428/1‑147 (MQ=255)
                                                                                tGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACg                                                              <  7:96550/149‑1 (MQ=255)
                                                                                     cGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGc                                                         <  4:93246/149‑1 (MQ=255)
                                                                                          gTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAAcgc                                                    <  2:333576/149‑1 (MQ=255)
                                                                                             ccAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATACGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgca                                                 <  5:202672/149‑1 (MQ=255)
                                                                                                 aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcacgca                                             >  5:218416/1‑149 (MQ=255)
                                                                                                 aaaGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTATATCCAGCTGAGCTACGAGTGCAACGcgcacgca                                             >  7:310106/1‑149 (MQ=255)
                                                                                                   aGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATg                                           <  6:218416/149‑1 (MQ=255)
                                                                                                    ggTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTTATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGa                                          <  6:291003/149‑1 (MQ=255)
                                                                                                      tGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATa                                        <  6:2423/149‑1 (MQ=255)
                                                                                                           tGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCAt                                   <  3:118380/149‑1 (MQ=255)
                                                                                                                  caAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTc                            <  2:189327/149‑1 (MQ=255)
                                                                                                                   aaaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcac                                                <  7:78848/128‑1 (MQ=255)
                                                                                                                   aaaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGcgcac                                                >  8:78848/1‑128 (MQ=255)
                                                                                                                    aaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGt                          >  2:86322/1‑149 (MQ=255)
                                                                                                                    aaCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGt                          <  1:154783/149‑1 (MQ=255)
                                                                                                                     aCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGtt                         <  7:183317/149‑1 (MQ=255)
                                                                                                                     aCGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGtt                         <  8:143106/149‑1 (MQ=255)
                                                                                                                        ataaaGCCCTGGGCCTGAGCCCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                      >  8:38271/3‑149 (MQ=39)
                                                                                                                        ataaaGCCCTGGGCCTGAGCCCGGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                      >  6:173524/3‑149 (MQ=39)
                                                                                                                        aaaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACGCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGAc                      <  6:389948/149‑1 (MQ=255)
                                                                                                                         aaaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACg                     <  8:131720/149‑1 (MQ=255)
                                                                                                                          aaaGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGt                    <  1:343637/149‑1 (MQ=255)
                                                                                                                                  gggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGcc            <  4:198921/149‑1 (MQ=255)
                                                                                                                                   ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACGCATGTGTGTCGTTGACGTCGATGGCCt           >  4:23049/1‑149 (MQ=255)
                                                                                                                                   ggCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCt           >  4:28088/1‑149 (MQ=255)
                                                                                                                                      tCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGCCCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGa        >  2:175561/1‑149 (MQ=255)
                                                                                                                                         ggCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCt     <  6:312931/149‑1 (MQ=255)
                                                                                                                                          gCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCtg    >  2:120892/1‑149 (MQ=255)
                                                                                                                                            ccAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCtgtg  <  5:85787/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TGCGCATTCCTTTGCCAATCATTGTTCAGGGTTACCCGTTCGGGAAACGGGAAGACGCCCACGCTAACACCAATGACCCGTGGTACGGGAGTACCAGAAAGGTGACATGTCGCACAAACGAAAAAGCCCCGGGCTCAGGCCCAGGGCTTTATTGATATGGCGCACTCGGCGGGATTCGAACCCACGACCCCTGCCTTCGGAGGGCAGTACTCTATCCAGCTGAGCTACGAGTGCAACGCGCACGCATGATACCCATCTGTGTCGTTGACGTCCATCGCCTTGATCTGTG  >  NC_002947/255122‑255410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑