Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,770,396 C→G 7.7% A661A (GCC→GCG)  PP_3330 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,770,3960CG 7.7% 161.1 / 4.4 52A661A (GCC→GCG) PP_3330ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base G (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

AGACCATCGGCGGCGTGGTGTACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGACCAACACC  >  NC_002947/3770251‑3770543
                                                                                                                                                 |                                                                                                                                                   
agaCCATCGGCGGCGTGGTGTACTCGCGCAAGGGGAAGAAGCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGAACTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAac                                                                                                                                                  <  7:147495/149‑1 (MQ=255)
     aTCGGCGGCGTGGTGTACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGc                                                                                                                                             >  7:103054/1‑149 (MQ=255)
        ggcggcGTGGTGTACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTTGGCGTACACCgat                                                                                                                                            >  2:308503/1‑145 (MQ=255)
           ggcgTGGTGTACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTTGGCGTACACCgatgagac                                                                                                                                       <  1:308503/149‑8 (MQ=255)
           ggcgTGGTGTACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATAc                                                                                                                                       <  3:184235/149‑1 (MQ=255)
                 gtgtACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCAt                                                                                                                                  <  2:277117/148‑1 (MQ=255)
                 gtgtACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATg                                                                                                                                 <  7:1123/149‑1 (MQ=255)
                 gtgtACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATg                                                                                                                                 >  1:351550/1‑149 (MQ=255)
                    tACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATggg                                                                                                                               >  4:330884/1‑148 (MQ=255)
                      cTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTa                                                                                                                             <  7:306518/148‑1 (MQ=255)
                       tCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTAcc                                                                                                                           >  4:181647/1‑149 (MQ=255)
                                 gCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACAcc                                                                                                                 >  5:143571/1‑149 (MQ=255)
                                          cGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACggg                                                                                                        >  7:141818/1‑149 (MQ=255)
                                          cGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACggg                                                                                                        <  2:351550/149‑1 (MQ=255)
                                          cGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACggg                                                                                                        <  3:181647/149‑1 (MQ=255)
                                                  aTCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCAt                                                                                                <  6:143571/149‑1 (MQ=255)
                                                   tCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTTGGCGTACACCgatg                                                                                                                                           <  3:331619/105‑4 (MQ=255)
                                                   tCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTTGGCGTACACCgatg                                                                                                                                           >  4:331619/1‑102 (MQ=255)
                                                     cccGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGa                                                                                              <  5:99280/148‑1 (MQ=255)
                                                        gACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCa                                                                                          <  1:83464/149‑1 (MQ=255)
                                                                   cAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCCCGACCTGGAc                                                                                >  4:314777/1‑148 (MQ=255)
                                                                        tctGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCAc                                                                          >  5:46586/1‑149 (MQ=255)
                                                                               gACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCaa                                                                    <  8:141818/148‑1 (MQ=255)
                                                                                  cTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCa                                                                <  8:103054/149‑1 (MQ=255)
                                                                                    aCGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTcc                                                                                                  <  3:258376/113‑1 (MQ=255)
                                                                                    aCGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTcc                                                                                                  >  4:258376/1‑113 (MQ=255)
                                                                                    aCGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGTACAACCACGTGCAAGTCacc                                                              >  5:207560/1‑147 (MQ=255)
                                                                                     cGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAg                                                               <  7:297284/147‑1 (MQ=255)
                                                                                      gACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAgcgc                                                            >  5:41923/1‑149 (MQ=255)
                                                                                       aCCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAgcgcg                                                           >  1:334184/1‑149 (MQ=255)
                                                                                         cTGGCGCCGGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAgcgcgc                                                          <  8:270992/148‑1 (MQ=255)
                                                                                               cccGGCTGGCAGGTCGGGGCCGATGCCCGGTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGGATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTgcg                                                   >  2:371354/1‑149 (MQ=255)
                                                                                                         aGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCAcc                                         >  8:345108/1‑149 (MQ=255)
                                                                                                         aGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCAcc                                         >  6:177333/1‑149 (MQ=255)
                                                                                                         aGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCAcc                                         >  6:362401/1‑149 (MQ=255)
                                                                                                              ggggCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACcgacga                                    <  2:130568/149‑1 (MQ=255)
                                                                                                                ggCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAgg                                  <  6:46586/149‑1 (MQ=255)
                                                                                                                 gCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGgt                                 >  1:169711/1‑149 (MQ=255)
                                                                                                                  ccGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGgtg                                <  2:169711/149‑1 (MQ=255)
                                                                                                                         ccGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGccc                         >  8:123121/1‑149 (MQ=255)
                                                                                                                             gaTGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCtt                     <  7:365509/148‑1 (MQ=255)
                                                                                                                                gTCTCTTCGGTGTACGCCAACACCGCCAATACCGCTTGGGTACCGCCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTTGACAAGCACGTGCATGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCa                   <  6:207560/148‑1 (MQ=255)
                                                                                                                                 tctcATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACcg                                        >  7:61189/1‑126 (MQ=255)
                                                                                                                                 tctcATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACcg                                        <  8:61189/126‑1 (MQ=255)
                                                                                                                                 tctcATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATc                 >  2:49300/1‑149 (MQ=255)
                                                                                                                                   tcATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATcca               >  7:96567/1‑149 (MQ=255)
                                                                                                                                     aTCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAg                                   >  7:52494/1‑127 (MQ=255)
                                                                                                                                     aTCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAg                                   <  8:52494/127‑1 (MQ=255)
                                                                                                                                        ggTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAg           <  1:371354/148‑1 (MQ=255)
                                                                                                                                          tgtACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGAc         >  6:61167/1‑148 (MQ=255)
                                                                                                                                           gtACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGAcc        >  5:71160/1‑148 (MQ=255)
                                                                                                                                              cGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGACCAac     <  2:311631/148‑1 (MQ=255)
                                                                                                                                                ccAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGACCAACAcc  >  2:292004/1‑149 (MQ=255)
                                                                                                                                                ccAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGACCAACAcc  >  1:235749/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGACCATCGGCGGCGTGGTGTACTCGCGCAAGGGCAAGAACCCGGTCAACATCCCCGACCGCGTGGCCAACCTCTGGTTGACCTACGACCTGGCGCCCGGCTGGCAGGTCGGGGCCGATGCCCGCTATGTCTCATCGGTGTACGCCAACACCGCCAATACCGCATGGGTACCGTCGTACACCGTCTACGGGCTGTCCATGACCCACGACCTGGACAAGCACGTGCAAGTCAGCGCGCGGCTGCGCAACCTCACCGACGAGGTGTATGCCCGCTTCATCCACCAGACCAACACC  >  NC_002947/3770251‑3770543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑