Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,793,271 T→C 6.0% H16R (CAC→CGC)  PP_3354 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,793,2710TC 6.0% 193.2 / 2.6 66H16R (CAC→CGC) PP_3354acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base C (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

TGGCGATCGCCCGAACTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATAGCGCAAGT  >  NC_002947/3793130‑3793417
                                                                                                                                             |                                                                                                                                                  
tGGCGATCGCCCGAACTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTg                                                                                                                                                >  5:245908/1‑146 (MQ=255)
  gCGATCGCCCGAACTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCa                                                                                                                                              <  6:245908/146‑1 (MQ=255)
          ccGAACTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTg                                                                                                                                    >  8:143171/1‑148 (MQ=255)
               cTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTc                                                                                                                              <  3:356978/149‑1 (MQ=255)
               cTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTc                                                                                                                              <  7:239205/149‑1 (MQ=255)
                  ttCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTcgcg                                                                                                                           >  3:275017/1‑149 (MQ=255)
                   taaacggggCCAGCACCTGCTCGCAGACACGCGCCGCCTCTTCCAGTACCTGGGACTCAAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCATGCCTGATCGTCCAGCAGCCAGTGTTGCAGGACGAACTGCATGTcgcg                                                                                                                           <  6:368795/143‑1 (MQ=255)
                       aGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAggg                                                                                                                      <  1:94887/149‑1 (MQ=255)
                       aGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAggg                                                                                                                      <  5:36300/149‑1 (MQ=255)
                       aGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAggg                                                                                                                      >  2:94887/1‑149 (MQ=255)
                         gggCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGgc                                                                                                                    <  7:296759/149‑1 (MQ=255)
                             cAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCt                                                                                                                >  5:284367/1‑149 (MQ=255)
                             cAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCt                                                                                                                >  4:300440/1‑149 (MQ=255)
                                cACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTc                                                                                                              >  3:365991/1‑148 (MQ=255)
                                 aCCTGCTCCCATAACCGGCCCGCCTCTTCCAGACCCGGGGCCGCCCGGTGGTGGCCCACGGCGGCAAATGCCGGTAATTGCGCCCTGTCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCa                                                                                                            <  1:306449/149‑1 (MQ=255)
                                  ccTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAg                                                                                                           <  7:143171/149‑1 (MQ=255)
                                   cTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAgg                                                                                                          <  7:104115/149‑1 (MQ=255)
                                               aCGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATAcc                                                                                              >  2:95949/1‑149 (MQ=255)
                                                 gggCCCCCTCTTCCAGTACCTCGGCCGCCAGGTCGAGGTCGACGTCGGCATAAGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTAGCAGGACGAACTGCATCTCGCGCAGGGGCGCCTTCCAGGTCATCGGATAccg                                                                                             >  4:207687/1‑147 (MQ=255)
                                                   gccgccTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATAcc                                                                                              >  4:294079/1‑145 (MQ=255)
                                                   gccgccTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATAcc                                                                                              <  3:294079/145‑1 (MQ=255)
                                                   gccgccTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCAACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCg                                                                                          >  5:172761/1‑149 (MQ=255)
                                                       ccTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGTCTTCCAGGTCATCGGATACCTCCGGGTc                                                                                      <  1:24129/149‑1 (MQ=255)
                                                       ccTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTc                                                                                      <  6:284367/149‑1 (MQ=255)
                                                         tctTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAg                                                                                    >  3:226525/1‑149 (MQ=255)
                                                           ttCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAg                                                                                    <  1:95949/147‑1 (MQ=255)
                                                             ccAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAgg                                                                                   <  4:226525/146‑1 (MQ=255)
                                                             ccAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCaa                                                                                >  6:138983/1‑149 (MQ=255)
                                                                   ccTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAAt                                                                          >  5:45931/1‑149 (MQ=255)
                                                                   ccTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGCGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAAt                                                                          >  1:57888/1‑149 (MQ=255)
                                                                   ccTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATACCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGCGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAAt                                                                          >  1:63484/1‑149 (MQ=255)
                                                                      gggCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCGGGCAACGAAATGAg                                                                       >  4:335873/1‑149 (MQ=255)
                                                                       ggCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAg                                                                       <  3:245177/148‑1 (MQ=255)
                                                                        gccgccAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAggg                                                                     >  7:299317/1‑149 (MQ=255)
                                                                            ccAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGtttt                                                                 <  3:300440/149‑1 (MQ=255)
                                                                              aggtcgaggtcgACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCg                                                               >  8:143104/1‑149 (MQ=255)
                                                                               ggtcgaggtcgaCGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGa                                                              <  6:45931/149‑1 (MQ=255)
                                                                                 tcgaggtcgaCGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGa                                                              <  1:137547/147‑1 (MQ=255)
                                                                                 tcgaggtcgaCGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGa                                                              >  2:137547/1‑147 (MQ=255)
                                                                                      gtcgaCGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTT‑CAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCagcagc                                                      >  3:18791/1‑149 (MQ=255)
                                                                                              cGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGc                                               >  8:365967/1‑149 (MQ=255)
                                                                                               ggCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGcc                                              <  8:299317/149‑1 (MQ=255)
                                                                                                      gCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCa                                       >  2:330075/1‑149 (MQ=255)
                                                                                                       ccGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCAc                                      >  4:325028/1‑149 (MQ=255)
                                                                                                          gTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATc                                                                                                     >  4:106143/1‑83 (MQ=255)
                                                                                                          gTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATc                                                                                                     <  3:106143/83‑1 (MQ=255)
                                                                                                           tAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGCGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCAccgcc                                  >  8:115731/1‑149 (MQ=255)
                                                                                                              ttGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCTCCGCCGat                               <  6:140922/149‑1 (MQ=255)
                                                                                                                  tcccAGGGCTGATCGGCGCGCAGCCAGTGTTGCAGGACGATCTGGATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCATCGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGatgcat                           <  3:207687/148‑1 (MQ=255)
                                                                                                                  gcCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGatgcat                           >  7:202850/1‑149 (MQ=255)
                                                                                                                  gcCCAGGCCTGATCGGCCCGCAGCCAGCGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGatgcat                           <  2:57888/149‑1 (MQ=255)
                                                                                                                  gcCCAGGCCTGATCGGCCCGCAGCCAGCGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGatgcat                           <  2:63484/149‑1 (MQ=255)
                                                                                                                    ccAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGatgcatgc                         >  2:308909/1‑149 (MQ=255)
                                                                                                                          cTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGGCATGCATCGGCGGTGGCCAAGGCATGGCGCTGc                   <  4:275017/149‑1 (MQ=37)
                                                                                                                            gATCGGCCCGCAGCCAGTGTTGCAGGCCGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGCTACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGACGCATGCCGCTGCGa                 >  2:303156/1‑149 (MQ=255)
                                                                                                                             aTCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGAt                >  3:355349/1‑149 (MQ=255)
                                                                                                                             aTCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGAt                <  1:330075/149‑1 (MQ=255)
                                                                                                                                   ccGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCa            >  6:112447/1‑147 (MQ=255)
                                                                                                                                   ccGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATa          <  6:172761/149‑1 (MQ=255)
                                                                                                                                   ccGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATa          <  5:138983/149‑1 (MQ=255)
                                                                                                                                    cGCAGCCAGTGTTGCAGGCCGAACTGCATGTCGCGCAGGGGCGCCGTCCAGGTCATCGGATCCCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATAg         <  7:135610/149‑1 (MQ=255)
                                                                                                                                       aGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATAgcgc      <  8:202850/149‑1 (MQ=255)
                                                                                                                                         ccAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCAt                                                 <  7:182342/104‑1 (MQ=255)
                                                                                                                                         ccAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCAt                                                 >  8:182342/1‑104 (MQ=255)
                                                                                                                                          cAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATAGCGCAAg   >  2:157975/1‑149 (MQ=255)
                                                                                                                                           aGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATAGCGCAAGt  >  3:174011/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TGGCGATCGCCCGAACTGTTCAAAGGGGCCAGCACCTGCTCGCAGAAACGGGCCGCCTCTTCCAGTACCTGGGCCGCCAGGTCGAGGTCGACGTCGGCATATGCCGGTAATTGCGCCCAGGCCTGATCGGCCCGCAGCCAGTGTTGCAGGACGAACTGCATGTCGCGCAGGGGCGCCTTCCAGGTCATCGGATACCTCCGGGTCAGGCAACGAAATGAGGGTTTTCGATCAGCAGCGCCATGCCTTGGCCACCGCCGATGCATGCCGCTGCGATGCCATAGCGCAAGT  >  NC_002947/3793130‑3793417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑