Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,793,897	A→T	7.5%	I209N (ATT→AAT)	PP_3355 ←	beta‑ketothiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,793,897	0	A	T	7.5%	145.6 / 3.1	53	I209N (ATT→AAT)	PP_3355	beta‑ketothiolase
Reads supporting (aligned to +/- strand):  ref base A (22/27);  new base T (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

TGCCGCCGCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCA  >  NC_002947/3793749‑3794043                                                                                                                                                     |                                                                                                                                                   tGCCGCCGCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGgcaatgcaa                                                                                                                                                    >  2:14593/1‑149 (MQ=255)   ccgccgCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGcaatgcaatg                                                                                                                                                  <  4:308787/149‑1 (MQ=255)    cgccgcGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCaatgcaatgc                                                                                                                                                 <  5:88002/149‑1 (MQ=255)         cgcCATCGACCACCGCGCAACTGTTGCCAGCCGGCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGt                                                                                                                                            <  5:119107/149‑1 (MQ=255)          gcCTTCGACCACCGGGCCTCTGTTGCGAGCCGTCTGCCCGCCACCGGCGTGCTCCGGGCGCAGTCGGGGCAGTGCGGCGGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTGACTCACGCCAGGCGGCATTGCATTGCCGCGtg                                                                                                                                           <  8:331648/149‑1 (MQ=255)          gcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtg                                                                                                                                           <  1:98602/149‑1 (MQ=255)          gcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtg                                                                                                                                           <  8:242412/149‑1 (MQ=255)            cATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCATTGCATTGCCGCGtggc                                                                                                                                         <  6:134930/149‑1 (MQ=255)                gACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCATGCCACGCGGCAATGCAATGCCGCGtggctgg                                                                                                                                      >  1:337522/1‑148 (MQ=255)                gACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggctgg                                                                                                                                      <  3:38212/148‑1 (MQ=255)                 accaccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggctgg                                                                                                                                      >  2:375937/1‑147 (MQ=255)                 accaccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCa                                                                                                                                    <  1:347083/149‑1 (MQ=255)                           aaCTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTAcc                                                                                                                          <  1:381551/149‑1 (MQ=255)                           aaCTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTAcc                                                                                                                          <  6:275084/149‑1 (MQ=255)                                ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCa                                                                                                                      <  6:141529/148‑1 (MQ=255)                                ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAg                                                                                                                     <  4:26155/149‑1 (MQ=255)                                  gCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGt                                                                                                                    <  1:19953/148‑1 (MQ=255)                                   ccAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCCGTGc                                                                                                                  >  3:244563/1‑149 (MQ=255)                                     aGCTGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGGGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCGACGCGGCAATGCAATGCCGCGTGTCTGGCAGCCTTCTACCTCCAGTGCCt                                                                                                                <  1:359874/149‑1 (MQ=255)                                         gTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTgcgc                                                                                                            <  5:334174/149‑1 (MQ=255)                                          ggtGCACGCCGGTGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGCCCTCACTCACCCCACCCGGCAATGCAATTCCGCCTGGGTGGCAGCCGTCTACCTCCCGTGCCTGcgcg                                                                                                           >  2:386140/3‑149 (MQ=255)                                              cACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGa                                                                                                       >  5:23729/1‑149 (MQ=255)                                              cACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGa                                                                                                       >  1:253081/1‑149 (MQ=255)                                                cGCCACCGGCGTGCACCGGGCGCAGTAGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACg                                                                                                     <  8:158787/149‑1 (MQ=255)                                                        gCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGCCGCTGCCGAt                                                                                             >  3:226569/1‑149 (MQ=255)                                                              aCCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtc                                                                                       >  1:180121/1‑149 (MQ=255)                                                              aCCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtc                                                                                       >  3:310615/1‑149 (MQ=255)                                                              aCCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtc                                                                                       <  7:203869/149‑1 (MQ=255)                                                                cGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                     <  4:237036/149‑1 (MQ=255)                                                                             gCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTg                                                                        <  1:14593/149‑1 (MQ=255)                                                                                aGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAc                                                                     >  5:97152/1‑149 (MQ=255)                                                                                            gcgggccgCCGTGGGTGGCGGGCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAg                                                         <  4:226569/142‑1 (MQ=255)                                                                                               gTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGc                                                      >  2:283871/1‑149 (MQ=255)                                                                                               gTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGc                                                      >  2:280971/1‑149 (MQ=255)                                                                                                    ccGTTGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAc                                                                     <  2:147282/129‑1 (MQ=255)                                                                                                    ccGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAc                                                                     >  1:147282/1‑129 (MQ=255)                                                                                                       tgggtggCTGTCCTGGCTGACCTCACTCATGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGAt                                              <  2:337522/149‑1 (MQ=255)                                                                                                       tgggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGAt                                              <  4:258933/149‑1 (MQ=255)                                                                                                         ggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATgg                                            >  6:210188/1‑149 (MQ=255)                                                                                                               tGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTgcg                                       >  2:382174/1‑148 (MQ=255)                                                                                                                   cTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAgg                                   <  1:283871/148‑1 (MQ=255)                                                                                                                   cTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGGGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAggg                                  <  4:111622/149‑1 (MQ=255)                                                                                                                         gACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCAcg                            >  7:101478/1‑149 (MQ=255)                                                                                                                           ccTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGa                                               <  4:219007/128‑1 (MQ=255)                                                                                                                           ccTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGa                                               >  3:219007/1‑128 (MQ=255)                                                                                                                            ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGt                         >  4:201090/1‑149 (MQ=255)                                                                                                                                ctcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCAc                     <  2:253081/149‑1 (MQ=255)                                                                                                                                ctcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCAc                     <  6:23729/149‑1 (MQ=255)                                                                                                                                 tcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCAcc                    >  7:200948/1‑149 (MQ=255)                                                                                                                                 tcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCAcc                    >  7:195661/1‑149 (MQ=255)                                                                                                                                   acgccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCAcc                    <  8:92861/147‑1 (MQ=255)                                                                                                                                   acgccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGt                  <  1:375937/149‑1 (MQ=255)                                                                                                                                   acgccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGt                  >  6:151545/1‑149 (MQ=255)                                                                                                                                       cacgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCa              >  3:237436/1‑149 (MQ=255)                                                                                                                                        acgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                 >  2:201931/1‑97 (MQ=255)                                                                                                                                          gcgGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCAcgc           >  2:290401/1‑149 (MQ=255)                                                                                                                                              caatgcaatgCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACgcgc         >  3:61977/1‑147 (MQ=255)                                                                                                                                              caatgcaatgCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACgcgc         >  6:101221/1‑147 (MQ=255)                                                                                                                                               aatgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCTCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAg      <  1:280971/149‑1 (MQ=255)                                                                                                                                                 tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGGGCAGGGCCCACGCGTCCACCGTTTCCCGCGCCAGGc    >  6:14044/1‑149 (MQ=255)                                                                                                                                                 tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGc    <  4:310615/149‑1 (MQ=255)                                                                                                                                                 tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGc    <  8:195661/149‑1 (MQ=255)                                                                                                                                                 tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGc    <  8:200948/149‑1 (MQ=255)                                                                                                                                                    aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGAGGATTTCCTCGTCGAACCCGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCa  >  7:159438/1‑148 (MQ=255)                                                                                                                                                    aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAg      >  3:223395/1‑144 (MQ=255)                                                                                                                                                    aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAg      <  4:223395/144‑1 (MQ=255)                                                                                                                                                    aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCa  >  6:302558/1‑148 (MQ=255)                                                                                                                                                    aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCa  >  3:166734/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TGCCGCCGCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCA  >  NC_002947/3793749‑3794043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑