Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,804,021	C→G	8.2%	intergenic (‑105/‑39)	PP_3361 ← / → PP_3362	aminotransferase/AsnC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,804,021	0	C	G	8.2%	181.1 / 7.2	63	intergenic (‑105/‑39)	PP_3361/PP_3362	aminotransferase/AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/35);  new base G (2/3);  total (24/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-02

GCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAGTGC  >  NC_002947/3803879‑3804166                                                                                                                                                |                                                                                                                                                  gCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCTGACCgg                                                                                                                                             >  5:323787/1‑149 (MQ=255) gCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCg                                                                                                                                              <  3:327009/148‑1 (MQ=255)  ccGAAAGCCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCTGAGCGCGCGGATTGTTTTTCAATAGCTTGTCTTGCGCCGCCGAAAGGGACCCCCTGCATAGGGTCGGGCGCAACAGCCATTATGCCGGTTCCGCCCgg                                                                                                                                             >  1:290435/1‑149 (MQ=255)  ccGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCgg                                                                                                                                             >  3:117405/1‑148 (MQ=255)  ccGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGc                                                                                                                                            <  1:149772/149‑1 (MQ=255)    gAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTCGGAACCGGCAt                                                                                                                                          <  2:388556/149‑1 (MQ=255)      aaaCCGAGGTGCCGTGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGGTTGTCATGCGCCGCCGAAACGGACACCCTGCATTCGGTCGGGCGCAACAGGCATTATGCCGGTCGGAACCGGCATa                                                                                                                                         <  6:6642/148‑1 (MQ=255)        aCCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTCGGAACCGGCATAATg                                                                                                                                      <  4:159488/149‑1 (MQ=255)          cGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGAc                                                                                                                                    >  2:159147/1‑149 (MQ=255)           gAGGTGCCGGGTGGGAAAGGGCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACg                                                                                                                                   <  4:127467/149‑1 (MQ=255)                gCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAg                                                                                                                              <  6:106146/149‑1 (MQ=255)                gCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAg                                                                                                                              <  2:9406/149‑1 (MQ=255)                           aaGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAgg                                                                                                                    <  8:195347/148‑1 (MQ=255)                                     atatCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGc                                                                                                         >  1:103317/1‑149 (MQ=255)                                        tcatcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCTGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGt                                                                                                       <  6:323787/148‑1 (MQ=255)                                        tcatcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGt                                                                                                       <  2:220240/148‑1 (MQ=255)                                         catcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGt                                                                                                       >  5:21437/1‑147 (MQ=255)                                         catcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGt                                                                                                       <  6:21437/147‑1 (MQ=255)                                          atcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGa                                                                                                     <  6:34738/148‑1 (MQ=255)                                          atcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGa                                                                                                     >  5:34738/1‑148 (MQ=255)                                           tcaGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTACAATGCGTGAcc                                                                                                   >  7:132067/1‑149 (MQ=255)                                             aGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTg                                                                                                 >  2:20291/1‑149 (MQ=255)                                             aGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTg                                                                                                 <  1:171625/149‑1 (MQ=255)                                             aGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTg                                                                                                 <  1:165893/149‑1 (MQ=255)                                               gTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGa                                                                                               <  1:370649/149‑1 (MQ=255)                                                          cgcTGATTGTTTTTCAATAGCTTGTCATGCCCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTa                                                                                          >  1:33933/1‑144 (MQ=255)                                                           gcTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATc                                                                                    <  2:103317/149‑1 (MQ=255)                                                               aTTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATcgc                                                                                  <  8:147246/147‑1 (MQ=255)                                                               aTTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGATGCAATGCGTGACCTGGACGCTAAAGATcgcg                                                                                 <  6:312208/148‑1 (MQ=255)                                                                        cAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTg                                                                        <  1:97377/148‑1 (MQ=255)                                                                         aaTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATaa                                                                                                                                        >  7:162460/1‑83 (MQ=255)                                                                         aaTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATaa                                                                                                                                        <  8:162460/83‑1 (MQ=255)                                                                         aaTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAg                                                                      >  5:104559/1‑149 (MQ=255)                                                                             gCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTAc                                                                  <  8:182025/149‑1 (MQ=255)                                                                              cTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACt                                                                 >  1:344917/1‑149 (MQ=255)                                                                                  tCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGAATTATGCCGGTTCCGACCGGGATAATGACGGCAAGCTTATCTAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTc                                                              <  8:226269/148‑1 (MQ=255)                                                                                    aTGCGCCGCCGAAACGGACACCCTGCATACGTTCGGGCGCAACAGTCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGTTAAAGATCGCGAGATTCTTGAGGTACTTTCGaa                                                           <  1:249682/149‑1 (MQ=255)                                                                                          cgccgAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAcgc                                                     >  4:381191/1‑149 (MQ=255)                                                                                          cgccgAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAcgc                                                     <  7:79052/149‑1 (MQ=255)                                                                                            ccgAAAAGGAGACCCTGCATACGGTCGGGCGCAACATGCATTATGCCGGTTCCGACCGGGATATTGACGGCAAGCTTATCTAGGGGCTGGAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAcgcgc                                                   <  4:237616/149‑1 (MQ=255)                                                                                                       acCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTc                                        <  2:290435/149‑1 (MQ=255)                                                                                                            gCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTAtt                                              >  4:113588/1‑138 (MQ=255)                                                                                                            gCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTAtt                                              <  3:113588/138‑1 (MQ=255)                                                                                                               tACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGtt                                >  2:222948/1‑149 (MQ=255)                                                                                                                aCGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGt                                 >  8:48455/1‑147 (MQ=255)                                                                                                                 cGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTa                               <  4:117405/148‑1 (MQ=255)                                                                                                                 cGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAg                              <  6:227725/149‑1 (MQ=255)                                                                                                                  ggTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAgc                             >  5:370723/1‑149 (MQ=255)                                                                                                                    tCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAgccg                           >  5:219692/1‑149 (MQ=255)                                                                                                                            aaCAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCa                     >  5:153946/1‑147 (MQ=255)                                                                                                                            aaCAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATa                   <  1:20291/149‑1 (MQ=255)                                                                                                                              cAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCATGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGGGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAgg                 <  8:184405/149‑1 (MQ=255)                                                                                                                              cAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAgg                 <  1:159147/149‑1 (MQ=255)                                                                                                                              cAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGC‑TATCAAGGGGCTTCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGc                <  2:102391/149‑1 (MQ=255)                                                                                                                              cAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGC‑TATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATTGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGc                <  4:72889/149‑1 (MQ=255)                                                                                                                                 tcATTATGCCGGTCGGAACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCt              >  6:261362/2‑149 (MQ=255)                                                                                                                                 tcATTATGCCGGTCGGAACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGAGTGACCTGTACGCTAAATATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCt              >  8:284470/2‑149 (MQ=255)                                                                                                                                    ttATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTc           <  6:219692/149‑1 (MQ=255)                                                                                                                                     tATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTcc          >  2:274583/1‑149 (MQ=255)                                                                                                                                          cGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTgcg                                           >  1:132647/1‑111 (MQ=255)                                                                                                                                          cGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTgcg                                           <  2:132647/111‑1 (MQ=255)                                                                                                                                           ggTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAGt    <  2:33933/149‑1 (MQ=255)                                                                                                                                             ttCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTACAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAGTGc  <  8:132067/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  GCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCC‑GAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAGTGC  >  NC_002947/3803879‑3804166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑