Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,810,625 A→G 6.2% T248A (ACG→GCG)  PP_3366 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,810,6250AG 6.2% 183.3 / 2.4 64T248A (ACG→GCG) PP_3366LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (29/31);  new base G (2/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

CGCCATCTTCTGGATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGCCAGT  >  NC_002947/3810484‑3810773
                                                                                                                                             |                                                                                                                                                    
cGCCATCTTCTGGATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGGGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAgg                                                                                                                                               <  7:87017/149‑1 (MQ=255)
   cATCTTCTGGATGCGTTCTTCGCCGAACATGGCATCACTTACAAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCAt                                                                                                                                            >  4:183678/1‑149 (MQ=255)
         ctGGATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCAtcgtcg                                                                                                                                       <  8:205883/148‑1 (MQ=255)
         ctGGATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCAtcgt                                                                                                                                         <  2:130146/146‑1 (MQ=255)
           ggATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCg                                                                                                                                    >  1:245843/1‑149 (MQ=255)
            gATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGt                                                                                                                                   <  8:37611/149‑1 (MQ=255)
                    cttcGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGcgttgct                                                                                                                           >  6:277321/1‑143 (MQ=255)
                     ttcGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCg                                                                                                                          >  7:56237/1‑149 (MQ=255)
                      tcGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCgg                                                                                                                         >  6:111530/1‑149 (MQ=255)
                       cGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTcc                                                                                                                           <  8:243185/146‑1 (MQ=255)
                       cGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCg                                                                                                                          <  4:248803/147‑1 (MQ=255)
                        gCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGc                                                                                                                        >  2:356596/1‑148 (MQ=255)
                         ccGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGc                                                                                                                        >  3:8010/1‑147 (MQ=255)
                         ccGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCca                                                                                                                      <  5:111530/149‑1 (MQ=255)
                          cGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCcag                                                                                                                     <  3:69502/149‑1 (MQ=255)
                          cGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCcag                                                                                                                     <  3:183678/149‑1 (MQ=255)
                             aCATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCcagca                                                                                                                   >  8:262434/1‑148 (MQ=255)
                                tGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCcagcactg                                                                                                                >  4:340267/1‑146 (MQ=255)
                                 ggCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCcagcacag                                                                                                                >  7:298311/1‑147 (MQ=255)
                                    aTCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGAt                                                                                                           <  7:184172/149‑1 (MQ=255)
                                    aTCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGAt                                                                                                           <  7:262434/149‑1 (MQ=255)
                                    aTCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGAt                                                                                                           >  3:254546/1‑149 (MQ=255)
                                        cTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGt                                                                                                       <  1:356596/149‑1 (MQ=255)
                                              aGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCg                                                                                                                          >  3:221841/1‑124 (MQ=255)
                                              aGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCg                                                                                                                          <  4:221841/124‑1 (MQ=255)
                                              aGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACTGCAGATGAGTTTCCGc                                                                                                 <  3:340267/149‑1 (MQ=255)
                                                gTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCtt                                                                                               <  8:56237/149‑1 (MQ=255)
                                                gTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCACGGTGCAGCAACGCACGCCACGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCtt                                                                                               >  2:342488/1‑149 (MQ=255)
                                                   gcgcTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTtgtg                                                                                            <  4:34868/149‑1 (MQ=255)
                                                      cTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTg                                                                                         <  3:102245/149‑1 (MQ=255)
                                                       tACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTgg                                                                                        >  1:92042/1‑149 (MQ=255)
                                                               aaCTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCggg                                                                                <  2:245843/149‑1 (MQ=255)
                                                                aCTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCgggg                                                                               >  4:294608/1‑149 (MQ=255)
                                                                aCTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCgggg                                                                               <  2:92042/149‑1 (MQ=255)
                                                                  ttCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGc                                                                                                                                 <  6:182047/97‑1 (MQ=255)
                                                                  ttCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGc                                                                                                                                 >  5:182047/1‑97 (MQ=255)
                                                                       ctcGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCGCGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATAcc                                                                        >  1:15139/1‑149 (MQ=255)
                                                                          gCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTg                                                                      <  8:260525/148‑1 (MQ=255)
                                                                           cTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTc                                                                    >  4:366534/1‑149 (MQ=255)
                                                                                 gtgCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGc                                                              >  2:248417/1‑149 (MQ=255)
                                                                                 gtgCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGc                                                              >  6:282069/1‑149 (MQ=255)
                                                                                   gCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGAGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGc                                                              >  2:94545/1‑147 (MQ=255)
                                                                                     aGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCgggg                                                                               >  3:382330/1‑128 (MQ=255)
                                                                                        ccGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAg                                                       <  5:394149/149‑1 (MQ=255)
                                                                                         cgcgCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGc                                                      <  8:298311/149‑1 (MQ=255)
                                                                                            gcTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCGCGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGt                                                   >  8:348981/1‑149 (MQ=255)
                                                                                             cTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCt                                                                       >  4:291835/1‑128 (MQ=255)
                                                                                             cTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCt                                                                       <  3:291835/128‑1 (MQ=255)
                                                                                                gACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCAt                                               >  5:114818/1‑149 (MQ=255)
                                                                                                 acaacaTGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCGCGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATc                                              <  7:348981/149‑1 (MQ=255)
                                                                                                     caTGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACt                                          <  3:294608/149‑1 (MQ=255)
                                                                                                      aTGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGTGGAATAGCTGTCGCTGGCCAGCAAGCGGTTCATCGACtt                                         <  1:342488/149‑1 (MQ=255)
                                                                                                         ggggTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCGCGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTtgc                                      <  2:15139/149‑1 (MQ=255)
                                                                                                         ggggTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTtg                                       <  1:248417/148‑1 (MQ=255)
                                                                                                                 gACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTtgct                                     >  3:83634/1‑142 (MQ=255)
                                                                                                                 gACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTtgct                                     <  4:83634/142‑1 (MQ=255)
                                                                                                                 gACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGa                              >  8:353950/1‑149 (MQ=255)
                                                                                                                    ccTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACg                            >  1:56400/1‑148 (MQ=255)
                                                                                                                         tgcGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTAc                      >  7:230921/1‑149 (MQ=255)
                                                                                                                            gcTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTg                   <  7:353950/149‑1 (MQ=255)
                                                                                                                                   gcagcaACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAg                                                       >  6:996/1‑106 (MQ=255)
                                                                                                                                   gcagcaACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAg                                                       <  5:996/106‑1 (MQ=255)
                                                                                                                                     agcaACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGt            >  8:294835/1‑147 (MQ=255)
                                                                                                                                      gcaACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAg         <  5:349439/149‑1 (MQ=255)
                                                                                                                                         acgcacgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGc      <  4:8010/149‑1 (MQ=255)
                                                                                                                                          cgcacgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGc      >  2:180581/1‑148 (MQ=255)
                                                                                                                                          cgcacgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGc      >  3:95478/1‑148 (MQ=255)
                                                                                                                                           gcacgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACttt                                        <  2:236850/113‑1 (MQ=255)
                                                                                                                                           gcacgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACttt                                        >  1:236850/1‑113 (MQ=255)
                                                                                                                                           gcacgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGCCa    <  6:114818/149‑1 (MQ=255)
                                                                                                                                             acgcCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGCCAGt  <  4:95478/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CGCCATCTTCTGGATGCGTTCTTCGCCGAACATGGCATCACTTACCAGGTGGCGCTACAGAGCAACTTCGTCTCGCTGGTGGTGCAGGCCGCGCTGGACAACATGGGGGTGGCGACCCTGCTGCGCTCGGTGCAGCAACGCACGCCAGGCATCGTCGGCGTGCCGTTCCGGCCAGCACAGCAGATGAGTTTCCGCTTGTGCTGGCGCTCGGGGGAATACCTGTCGCTGGCCAGCAAGCGGTTCATCGACTTTGCTGCACAGACGCATTACCTGGAGCGGTGAGGGCCAGT  >  NC_002947/3810484‑3810773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑