Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 62,075 A→G 8.7% Y107C (TAT→TGT)  PP_0053 → sulfide:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294762,0750AG 8.7% 123.3 / 3.0 46Y107C (TAT→TGT) PP_0053sulfide:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base G (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-01

TCGGTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTCAAGC  >  NC_002947/61928‑62221
                                                                                                                                                   |                                                                                                                                                  
tCGGTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTAt                                                                                                                                                   <  1:245455/149‑1 (MQ=255)
tCGGTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTAt                                                                                                                                                   <  1:282631/149‑1 (MQ=255)
  ggTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGa                                                                                                                                                 <  4:380516/149‑1 (MQ=255)
   gTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGaa                                                                                                                                                <  3:335032/149‑1 (MQ=255)
    tGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCTTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCTGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAAc                                                                                                                                               <  8:98759/149‑1 (MQ=255)
       cGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGc                                                                                                                                            >  3:343386/1‑149 (MQ=255)
          tgtgtTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGa                                                                                                                                         >  6:169917/1‑149 (MQ=255)
              ttCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTGTTCATAGCTGAtggc                                                                                                                                     <  2:185501/149‑4 (MQ=255)
              ttCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCg                                                                                                                                      >  8:320415/1‑148 (MQ=255)
               tCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTGTTCATAGCTGAtggc                                                                                                                                     <  1:356600/148‑4 (MQ=255)
                     cACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGcccc                                                                                                                              >  8:287970/1‑149 (MQ=255)
                      aCCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGAt                                                                                                                                        >  2:345441/1‑138 (MQ=255)
                      aCCAAGCACTGCCCGCACCATCGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGAt                                                                                                                                        <  1:345441/138‑1 (MQ=255)
                         aaGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGcc                                                                                                                          >  3:75687/1‑149 (MQ=255)
                            cACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGa                                                                                                                                                 >  2:258575/1‑123 (MQ=255)
                            cACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGa                                                                                                                                                 <  1:258575/123‑1 (MQ=255)
                                            gCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGggcggc                                                                                                       >  3:11133/1‑149 (MQ=255)
                                                 gACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTg                                                                                                  <  8:348457/149‑1 (MQ=255)
                                                         cTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTc                                                                                          >  4:54187/1‑149 (MQ=255)
                                                          tCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCt                                                                                           >  3:180406/1‑147 (MQ=255)
                                                            gcgGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCa                                                                                         <  7:320415/147‑1 (MQ=255)
                                                             cgGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCg                                                                                      <  4:75687/149‑1 (MQ=255)
                                                              ggTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCga                                                                                     <  6:390565/149‑1 (MQ=255)
                                                                           tCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCACACGCTGGACTGGGCGGCCATTGATGGGATCAGCGAGACCCTGGGCCg                                                                         >  8:337499/1‑148 (MQ=255)
                                                                             aaGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCaa                                                                      <  4:343386/149‑1 (MQ=255)
                                                                                   cgGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGgtgt                                                                <  4:11133/149‑1 (MQ=255)
                                                                                      gTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCAc                                                             >  4:23850/1‑149 (MQ=255)
                                                                                            ggATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCaa                                                       >  2:183397/1‑149 (MQ=255)
                                                                                              aTTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAAc                                                      >  6:33736/1‑148 (MQ=255)
                                                                                                  gACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATc                                                  <  3:23850/148‑1 (MQ=255)
                                                                                                     ccATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGCCTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTa                                              >  6:125127/1‑149 (MQ=255)
                                                                                                     ccATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTa                                              >  2:274608/1‑149 (MQ=255)
                                                                                                      cATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTAc                                             <  7:287970/149‑1 (MQ=255)
                                                                                                             ccAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTgg                                      >  3:342824/1‑149 (MQ=255)
                                                                                                                    gTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCTCCCt                                <  5:330000/148‑1 (MQ=255)
                                                                                                                    gTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGACCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCta                               >  6:351391/1‑149 (MQ=255)
                                                                                                                                 atgGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                  <  1:183397/149‑1 (MQ=255)
                                                                                                                                    gTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGt                <  5:33736/148‑1 (MQ=255)
                                                                                                                                    gTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTg               <  1:274608/149‑1 (MQ=255)
                                                                                                                                       agacgaTCAGCTGTTCATAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGCCCCTGGGCCGCAACGGTGTCCCCTCCAAATATCGCTACGACCTGGAACCCTATACCTGGGAGCTGGTGCa             >  7:275973/6‑148 (MQ=255)
                                                                                                                                        gacgaTCAGCTGTTCATAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaa            >  7:101199/5‑148 (MQ=255)
                                                                                                                                         gacATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGTGCCGCAACTGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa          <  7:337499/147‑1 (MQ=255)
                                                                                                                                               aGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                  >  7:321823/1‑135 (MQ=255)
                                                                                                                                               aGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTg                  <  8:321823/135‑1 (MQ=255)
                                                                                                                                                 cTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaag   >  1:181368/1‑148 (MQ=255)
                                                                                                                                                  tATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTcaagc  >  3:163453/1‑148 (MQ=255)
                                                                                                                                                   aTGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa          <  6:11756/139‑1 (MQ=255)
                                                                                                                                                   aTGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCaaaa          >  5:11756/1‑139 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGGTGCCGGTGTGTTCAAGGCACCAAGCACTGCCCGCACCATGGCGTCGACCATTCCTCGCGGTGTGCGCTGGGTCAAGGCGCGGGTAAAGGGATTCGACCCATTGGCCCAGTTGGTCATCCTCGATGATGGTCGCGCCATCAGCTATGAACAGCTGATCGTCTGCCCCGGCCTCAAGCTGGACTGGGCGGCCATTGATGGGCTCAGCGAGACCCTGGGCCGCAACGGTGTCACCTCCAACTATCGCTACGACCTGGCACCCTATACCTGGGAGCTGGTGCAAAAGCTCAAGC  >  NC_002947/61928‑62221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑