Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,848,339 T→A 5.5% F35Y (TTC→TAC)  PP_3397 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,848,3390TA 5.5% 219.3 / 2.7 73F35Y (TTC→TAC) PP_3397hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/32);  new base A (2/2);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

CAGCGCCAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTGCGCCAGGCGCACCCGGGGCG  >  NC_002947/3848191‑3848484
                                                                                                                                                    |                                                                                                                                                 
cAGCGCCAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGtt                                                                                                                                                   >  3:15559/1‑149 (MQ=255)
cAGCGCCAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGtt                                                                                                                                                   >  2:321154/1‑149 (MQ=255)
cAGCGCCAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCAGTGCGCTGTTGGCCGCCGCTTTCTCTCTTACCGTTACCAGCGCCGCCtt                                                                                                                                                   >  5:265190/1‑149 (MQ=255)
     ccAACGGTTGTAGCGACTCCGAAGACGTTGTGCTGTTGCCATGATCAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAAGACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTAGCAGCGCCGCGTTcgcgg                                                                                                                                              <  2:277325/149‑3 (MQ=255)
     ccAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTcgccg                                                                                                                                              >  8:91526/1‑149 (MQ=255)
     ccAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTcgccg                                                                                                                                              >  8:198881/1‑149 (MQ=255)
      cAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTcgc                                                                                                                                                <  1:94183/146‑1 (MQ=255)
      cAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTcgc                                                                                                                                                >  2:94183/1‑146 (MQ=255)
       aaCGGTGGTAGCGACTACGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGATGCAGCAAGAGCACTTGGTCGCGCCGTGCGCTGATGGCCGCAGGAATCTCTCTAACCGTTACAAGCGCCGCGTTccaagcg                                                                                                                                            >  2:327890/1‑143 (MQ=255)
          ggtggtAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAACGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCGGCGAACGCGGCGCTg                                                                                                                                         <  8:331744/149‑1 (MQ=255)
           gtggtAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCGGCGAACGCGGCGCTgg                                                                                                                                        <  3:295011/149‑2 (MQ=255)
              gtAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGt                                                                                                                                        <  4:231576/146‑1 (MQ=255)
              gtAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGt                                                                                                                                        >  3:231576/1‑146 (MQ=255)
               tAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGa                                                                                                                                    >  1:99112/1‑149 (MQ=255)
                 gCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGAt                                                                                                                                   <  2:14289/148‑1 (MQ=255)
                 gCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATg                                                                                                                                  >  3:178192/1‑149 (MQ=255)
                  cGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGt                                                                                                                                 <  4:32163/149‑1 (MQ=255)
                     cTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGa                                                                                                                              >  8:248086/1‑149 (MQ=255)
                      tCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCt                                                                                                                                      >  4:71596/1‑140 (MQ=255)
                      tCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCt                                                                                                                                      <  3:71596/140‑1 (MQ=255)
                            gACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCTCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCgggg                                                                                                                       <  2:143536/149‑1 (MQ=255)
                              cGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGcc                                                                                                                     <  4:258887/149‑1 (MQ=255)
                                     ctggtgcCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTacac                                                                                                               <  4:178192/148‑1 (MQ=255)
                                      tggtgcCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGAGCCGCGTTCTCCGCGCTGTCTGATGTCGATCCGGGGCCCTaaccct                                                                                                             >  8:342577/1‑144 (MQ=255)
                                      tggtgcCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGAGCTGTTGGCCGCAGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGAGCAGTCTGATTTCGATCAGTGTCCCTAGACCt                                                                                                             >  8:337539/1‑149 (MQ=255)
                                        gtgcCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTc                                                                                                           <  3:210646/149‑1 (MQ=255)
                                           ccATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGTGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGc                                                                                                         >  1:177363/1‑148 (MQ=255)
                                           ccATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGc                                                                                                         >  7:7269/1‑148 (MQ=255)
                                             aTGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCca                                                                                                       >  1:371910/1‑148 (MQ=255)
                                                 aCAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCAGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGt                                                                                                  >  7:168652/1‑149 (MQ=255)
                                                  cAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTg                                                                                                 >  6:10866/1‑149 (MQ=255)
                                                   aGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGGACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTgg                                                                                                >  3:103128/1‑149 (MQ=255)
                                                    gACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTTGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGc                                                                                               <  1:321154/149‑1 (MQ=255)
                                                     aCAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGc                                                                                               >  4:118738/1‑148 (MQ=255)
                                                     aCAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGc                                                                                               >  1:73954/1‑148 (MQ=255)
                                                     aCAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGc                                                                                               >  4:169995/1‑148 (MQ=255)
                                                      cAAAGGCTCAGCCTTCAAGGAGGCAGCATGGACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTa                                                                                             <  4:103128/149‑1 (MQ=255)
                                                        aaGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATc                                                                                           <  2:371910/149‑1 (MQ=255)
                                                           gCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGa                                                                                        <  5:10866/149‑1 (MQ=255)
                                                             tCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGAtg                                                                                      >  7:157317/1‑149 (MQ=255)
                                                                gcagtCAAGGAGGCAGCATGATCACTTGGGCGCGCCGTGCGCTGGTGGCCGCCGGTTTCTCTCTTTCCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTgg                                                                                   <  4:81238/145‑1 (MQ=255)
                                                                  cTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGGTCCCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTa                                                                                 <  8:166365/149‑1 (MQ=255)
                                                                    tCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCcac                                                                                                      <  5:110362/126‑1 (MQ=255)
                                                                    tCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCcac                                                                                                      >  6:110362/1‑126 (MQ=255)
                                                                     cAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACag                                                                              >  7:343054/1‑148 (MQ=255)
                                                                      aaGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACaa                                                                              <  3:169995/148‑1 (MQ=255)
                                                                       aggaggCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAg                                                                             >  6:293614/1‑148 (MQ=255)
                                                                        ggaggCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGa                                                                           <  7:198881/149‑1 (MQ=255)
                                                                          atgcaccaTGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCAGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACa                                                                         <  7:342577/147‑1 (MQ=255)
                                                                          aggCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACa                                                                         <  4:15559/149‑1 (MQ=255)
                                                                           ggCAGCATGAACACTTGGTCGCGCCGTTCGCTGTTGGCCGCCTGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCTGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACa                                                                         <  7:337539/148‑1 (MQ=255)
                                                                             cagcaTGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGcc                                                                      <  7:91526/149‑1 (MQ=255)
                                                                              agcaTGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCt                                                                     >  2:65827/1‑149 (MQ=255)
                                                                                  tGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCGACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGAc                                                                 <  6:265190/149‑1 (MQ=255)
                                                                                  tGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGAc                                                                 <  5:293614/149‑1 (MQ=255)
                                                                                            gTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTgg                                                       <  7:248086/149‑1 (MQ=255)
                                                                                                cgcCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCt                                                   >  5:326956/1‑149 (MQ=255)
                                                                                                  ccGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCtgt                                                 <  1:65827/149‑1 (MQ=255)
                                                                                                            ttGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCACTCCCTGGAGCTGTGCCAGTcgcg                                       >  2:354636/1‑149 (MQ=255)
                                                                                                                ccgccgGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGcc                                   >  6:320185/1‑149 (MQ=255)
                                                                                                                  gccgGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGccac                                 >  4:170275/1‑149 (MQ=255)
                                                                                                                    cgGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGccacca                               >  8:319168/1‑149 (MQ=255)
                                                                                                                      gTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGc                             <  3:118738/149‑1 (MQ=255)
                                                                                                                        ttCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACaa                                                                              >  1:183509/1‑98 (MQ=255)
                                                                                                                        ttCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACaa                                                                              <  2:183509/98‑1 (MQ=255)
                                                                                                                              ctTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTgc                     >  8:11734/1‑149 (MQ=255)
                                                                                                                              ctTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACATTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTgc                     >  6:373639/1‑149 (MQ=255)
                                                                                                                                 aCCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACACGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTgc                     <  8:343054/146‑1 (MQ=255)
                                                                                                                                 aCCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTgcgc                   <  3:170275/148‑1 (MQ=255)
                                                                                                                                 aCCGTTACCAGCCCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCCCAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTCTGCCAGTCGCGTGCCACCACCACCCGTgaccc                  >  8:115498/1‑145 (MQ=255)
                                                                                                                                   cGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGTGCCCTACACATTCGCCACAGGTGTCTATCCGATGTGGTACAAGGACAGCCTCGGCCAATCCCTGGAGCTGTGCCa                                             >  5:266110/1‑120 (MQ=255)
                                                                                                                                   cGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTGCGCCAg                >  4:105758/1‑149 (MQ=255)
                                                                                                                                       gacAGCGCGGCGAACGCGGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTGCGCCAGgcgc            >  3:229199/3‑149 (MQ=255)
                                                                                                                                        acAGCGCGGCGAACGCGGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCATGGAGCTGTGCCAGTCGCGTGCCACCAGAACCCGTGCGCCAGGCGCa           >  7:326585/2‑149 (MQ=255)
                                                                                                                                                gcgTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTGCGCCAGGCGCACCCGGGgc   <  4:291726/149‑1 (MQ=255)
                                                                                                                                                 cgTTCGCCCCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCCCCACAGGTGGCTATCAGATGTGGAACAACGACAGCATAGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTGCGCCAGGCGCACCCggcccg  >  2:66160/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGCGCCAACGGTGGTAGCGACTCCGAAGACGTGGTGCTGGTGCCATGAACAGACAAAGGCTCAGCCTTCAAGGAGGCAGCATGAACACTTGGTCGCGCCGTGCGCTGTTGGCCGCCGGTTTCTCTCTTACCGTTACCAGCGCCGCGTTCGCCGCGCTGTCTGATGTCGATCCGGGGCCCTACACCTTCGCCACAGGTGGCTATCCGATGTGGTACAAGGACAGCCTCGACCAATCCCTGGAGCTGTGCCAGTCGCGTGCCACCAGCACCCGTGCGCCAGGCGCACCCGGGGCG  >  NC_002947/3848191‑3848484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑