Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,850,957 A→C 5.2% N147H (AAT→CAT)  PP_3398 → curli fiber surface‑exposed nucleator CsgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,850,9570AC 5.2% 249.6 / 3.5 77N147H (AAT→CAT) PP_3398curli fiber surface‑exposed nucleator CsgB
Reads supporting (aligned to +/- strand):  ref base A (36/37);  new base C (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

CAACAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGACG  >  NC_002947/3850816‑3851102
                                                                                                                                             |                                                                                                                                                 
cAACAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCa                                                                                                                                            <  5:14295/149‑1 (MQ=255)
 aaCAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACACCGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAg                                                                                                                                           >  6:186482/1‑149 (MQ=255)
 aaCAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAg                                                                                                                                           >  1:369749/1‑149 (MQ=255)
  aCAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACTGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGc                                                                                                                                          <  3:291673/149‑1 (MQ=255)
   cAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCa                                                                                                                                         >  6:75140/1‑149 (MQ=255)
    aGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCAt                                                                                                                                        >  5:197037/1‑149 (MQ=255)
    aGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCAt                                                                                                                                        >  4:79176/1‑149 (MQ=255)
     gggAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCATGCTGGCCATTGCCAGCCTg                                                                                                                                       <  4:67221/149‑1 (MQ=255)
       gAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGt                                                                                                                                      >  3:311948/1‑148 (MQ=255)
           tGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGtt                                                                                                                                     >  7:364367/1‑145 (MQ=255)
             gCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGAt                                                                                                                                >  6:230412/1‑148 (MQ=255)
             gCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGAt                                                                                                                                >  3:321174/1‑148 (MQ=255)
               aaCAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCAc                                                                                                                             <  5:103720/149‑1 (MQ=255)
                       gTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATAcc                                                                                                                     >  5:226603/1‑149 (MQ=255)
                       gTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATAcc                                                                                                                     <  6:52544/149‑1 (MQ=255)
                          gATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGa                                                                                                                   <  4:81265/148‑1 (MQ=255)
                          gATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGa                                                                                                                   >  7:354946/1‑148 (MQ=255)
                           aTTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGACTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGa                                                                                                                   >  7:110634/1‑147 (MQ=255)
                               gCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGAc                                                                                                              <  6:257533/148‑1 (MQ=255)
                                   gagCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGa                                                                                                         <  5:109591/149‑1 (MQ=255)
                                        gcagcagcaACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGcac                                                                                                    <  8:364367/149‑1 (MQ=255)
                                          agcagcaACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGcacca                                                                                                  <  8:354946/149‑1 (MQ=255)
                                            cagcaACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGcaccac                                                                                                 >  5:369945/1‑148 (MQ=255)
                                            cagcaACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCACGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGcaccac                                                                                                 >  7:266433/1‑148 (MQ=255)
                                              gcaACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCAt                                                                                              <  5:230412/149‑1 (MQ=255)
                                               caacaaCGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCAcc                                                                                                                            <  6:337158/118‑1 (MQ=255)
                                               caacaaCGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCAcc                                                                                                                            >  5:337158/1‑118 (MQ=255)
                                               caacaaCGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCAt                                                                                              >  7:302666/1‑148 (MQ=255)
                                                aacaacGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGt                                                                                            <  2:369749/149‑1 (MQ=255)
                                                 acaacGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTa                                                                                           >  8:333836/1‑149 (MQ=255)
                                                   aacGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACa                                                                                         <  4:311948/149‑1 (MQ=255)
                                                   aacGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAAGACCGATAGACCTGGAGGCACCACCTGGTAc                                                                                          >  6:35082/1‑148 (MQ=255)
                                                    acGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACaa                                                                                        >  5:141117/1‑149 (MQ=255)
                                                      gccgccATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCt                                                                                                            <  7:109450/127‑1 (MQ=255)
                                                      gccgccATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCt                                                                                                            >  8:109450/1‑127 (MQ=255)
                                                        cgccATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGACTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAgctc                                                                                    <  8:110634/149‑1 (MQ=255)
                                                          ccATCGAGCAGATCGGCAACGACAACAGCGCGAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAgctcgc                                                                                  <  1:193164/149‑1 (MQ=255)
                                                           cATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAgctcgct                                                                                 <  8:229311/149‑1 (MQ=255)
                                                           cATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAgctcgct                                                                                 <  8:187719/149‑1 (MQ=255)
                                                            aTCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAgctcgctc                                                                                <  8:302666/149‑1 (MQ=255)
                                                            aTCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGATCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAAAACCGATAGACCTGGAGGCACCACCATGTACAAtctcgctc                                                                                >  5:80306/1‑149 (MQ=255)
                                                                aGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGtt                                                                            <  7:333836/149‑1 (MQ=255)
                                                                   aGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAg                                                                         <  6:141117/149‑1 (MQ=255)
                                                                      tCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGc                                                                      >  2:123563/1‑149 (MQ=255)
                                                                           aaCGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTa                                                                 >  2:241048/1‑149 (MQ=255)
                                                                             cGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATc                                                               <  6:197037/149‑1 (MQ=255)
                                                                                caacaGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGATGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTc                                                            <  2:213198/149‑1 (MQ=255)
                                                                                caacaGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTc                                                            >  8:64534/1‑149 (MQ=255)
                                                                                    aGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTACAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTgg                                                        <  1:123563/149‑1 (MQ=255)
                                                                                         cAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTg                                                         >  3:286337/1‑143 (MQ=255)
                                                                                         cAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTg                                                         <  4:286337/143‑1 (MQ=255)
                                                                                                 tGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGt                                             >  1:347267/1‑147 (MQ=255)
                                                                                                  gCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAg                                          >  2:236681/1‑149 (MQ=255)
                                                                                                   cAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAg                                          <  6:226603/148‑1 (MQ=255)
                                                                                                    aGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCt                                                    <  8:125055/137‑1 (MQ=255)
                                                                                                    aGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCt                                                    >  7:125055/1‑137 (MQ=255)
                                                                                                        cGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCAt                                     <  2:347267/148‑1 (MQ=255)
                                                                                                        cGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCAt                                     <  3:79176/148‑1 (MQ=255)
                                                                                                        cGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGACAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCAt                                     <  7:320309/148‑1 (MQ=255)
                                                                                                         ggCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATgg                                   <  6:369945/149‑1 (MQ=255)
                                                                                                            tCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCa                                           >  6:150312/1‑138 (MQ=255)
                                                                                                            tCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCa                                           <  5:150312/138‑1 (MQ=255)
                                                                                                            tCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGgctg                                >  3:376586/1‑149 (MQ=255)
                                                                                                              aGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGgctgc                               <  7:64534/148‑1 (MQ=255)
                                                                                                              aGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGgctg                                <  5:186482/147‑1 (MQ=255)
                                                                                                                     aaCAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAg                         <  8:266433/147‑1 (MQ=255)
                                                                                                                     aaCAGCTCCGTGACCCAGGCTGGCAATGGCAAGCAAGATCAGATAACCAAATACAGATAGACCTGGAGGCACAACCATGTACAAGCTCCCTCCGTTAAGTGACGCTATCGTCCTGGCCCTCGCAGGACAGGCCATGGCTGCTGACAGCa                       >  6:278580/1‑149 (MQ=255)
                                                                                                                       cAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCAc                      >  4:340063/1‑148 (MQ=255)
                                                                                                                        aGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCt                    >  1:37397/1‑149 (MQ=255)
                                                                                                                              gTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTcc               >  5:137453/1‑148 (MQ=255)
                                                                                                                                gACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTcc               >  5:341908/1‑146 (MQ=255)
                                                                                                                                 gaacatgCTGGCCATTGCCAGCCTGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGa           >  5:87194/7‑149 (MQ=255)
                                                                                                                                  cccAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGAc          >  8:62492/1‑149 (MQ=255)
                                                                                                                                    catgCTGGCCATTGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGAcc         >  5:15543/4‑148 (MQ=255)
                                                                                                                                     atgCTGGCCATTGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCa       <  6:15543/147‑1 (MQ=255)
                                                                                                                                       gCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAgg     <  2:37397/149‑1 (MQ=255)
                                                                                                                                           gCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGACg  <  1:241048/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CAACAGGGAAATGGCAACAGTGCGTCGATTCGCCAGAGCGGCAGCAGCAACAACGCCGCCATCGAGCAGATCGGCAACGACAACAGCGCCAGCATCGTGCAGTCCGGCTCAGGGCTCAACAGCTCCGTGACCCAGGCTGGCAATGGCCAGCATGTTCAGATCACCCAATACCGATAGACCTGGAGGCACCACCATGTACAAGCTCGCTCCGTTAAGTGCCGCTATCGTCCTGGCCCTCGCAGGTCAGGCCATGGCTGCTGACAGCACCTCGTCCCAGACCCAGGACG  >  NC_002947/3850816‑3851102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑