Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 276,323 T→A 5.5% D282V (GAC→GTC)  PP_0223 ← DszC family monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947276,3230TA 5.5% 220.2 / 2.4 72D282V (GAC→GTC) PP_0223DszC family monooxygenase
Reads supporting (aligned to +/- strand):  ref base T (39/29);  new base A (2/2);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GCGGCGGCCACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAGGCGCTTT  >  NC_002947/276179‑276470
                                                                                                                                                |                                                                                                                                                   
gcggcggcCACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAg                                                                                                                                                 >  1:127564/1‑149 (MQ=255)
 cggcggcCACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAgg                                                                                                                                                <  1:112992/149‑1 (MQ=255)
  ggcggcCACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGc                                                                                                                                               <  3:148807/149‑1 (MQ=255)
   gcggcCACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGcc                                                                                                                                              >  3:81516/1‑149 (MQ=255)
       ccACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAg                                                                                                                                          >  2:117976/1‑149 (MQ=255)
        cACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGt                                                                                                                                         <  8:313465/149‑1 (MQ=255)
        cACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGt                                                                                                                                         >  2:201301/1‑149 (MQ=255)
            ttGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGAc                                                                                                                                     <  6:191595/149‑1 (MQ=255)
            ttGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGAc                                                                                                                                     <  2:127564/149‑1 (MQ=255)
             tGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGAcc                                                                                                                                    >  1:55715/1‑149 (MQ=255)
               tCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGCGGCCTTGTCCAGGCCGGAGTCGACCCa                                                                                                                                  >  6:386027/1‑149 (MQ=255)
                    aGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCCCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCacggac                                                                                                                             <  8:263179/149‑1 (MQ=255)
                         tGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTg                                                                                                                        >  3:35631/1‑149 (MQ=255)
                             gtcgCGGGCGCGGTCGAGGTATTCGCCTGCTCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCgg                                                                                                                     <  8:323192/148‑1 (MQ=255)
                              tcgCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGcc                                                                                                                                              >  6:49005/1‑122 (MQ=255)
                              tcgCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGcc                                                                                                                                              <  5:49005/122‑1 (MQ=255)
                              tcgCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGt                                                                                                                   >  1:249650/1‑149 (MQ=255)
                                gcgGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTgc                                                                                                                 <  2:322808/149‑1 (MQ=255)
                                gcgGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTgc                                                                                                                 >  1:322808/1‑149 (MQ=255)
                                  gggCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCg                                                                                                                      <  6:212284/142‑1 (MQ=255)
                                  gggCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCg                                                                                                                      >  5:212284/1‑142 (MQ=255)
                                       cgGTCGAGGTATTCGCCTGCGCGCTCCAGGAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGTCTGCGGGTGCGCACGaa                                                                                                          <  4:81516/149‑1 (MQ=255)
                                       cgGTCGAGGAATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGGGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGaa                                                                                                          <  3:40611/149‑1 (MQ=255)
                                             aGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAg                                                                                                    <  7:197895/149‑1 (MQ=255)
                                             aGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAg                                                                                                    <  2:249650/149‑1 (MQ=255)
                                             aGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAg                                                                                                    <  8:104553/149‑1 (MQ=255)
                                             aGGTATTCGCCTGCGCGCTCCAGCAAGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAg                                                                                                    >  8:215949/1‑149 (MQ=255)
                                              ggTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAg                                                                                                                                          >  5:376052/1‑110 (MQ=255)
                                              ggTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAg                                                                                                                                          <  6:376052/110‑1 (MQ=255)
                                              ggTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAgg                                                                                                   <  1:201301/149‑1 (MQ=255)
                                                             gcTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGCAGTGCAGGGCCTGGTCATAggag                                                                                    >  6:266545/1‑147 (MQ=255)
                                                              cTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAggcgg                                                                                   <  7:263559/149‑1 (MQ=255)
                                                               tCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGAc                                                                                                                                     <  7:311591/98‑1 (MQ=255)
                                                               tCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGAc                                                                                                                                     >  8:311591/1‑98 (MQ=255)
                                                               tCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAggcggc                                                                                  >  2:6577/1‑149 (MQ=255)
                                                                              gCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGgt                                                                   <  2:55715/149‑1 (MQ=255)
                                                                                    tGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGAt                                                             >  3:373444/1‑149 (MQ=255)
                                                                                    tGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGCCGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTGCATAGGCGGCGCGGGCAATACCGGTGTCGAt                                                             <  5:386027/149‑1 (MQ=255)
                                                                                       aaGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATggc                                                          >  5:270799/1‑149 (MQ=255)
                                                                                         gCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATggcgg                                                        <  1:117976/149‑1 (MQ=255)
                                                                                            gACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCAt                                                     >  3:162014/1‑149 (MQ=255)
                                                                                                gCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATggag                                                 >  7:350070/1‑149 (MQ=255)
                                                                                                gCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATggag                                                 >  3:182525/1‑149 (MQ=255)
                                                                                                 ccAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATggag                                                 >  8:231910/1‑148 (MQ=255)
                                                                                                         ccGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTgcgc                                        >  4:283946/1‑149 (MQ=255)
                                                                                                         ccGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTgcgc                                        >  4:81526/1‑149 (MQ=255)
                                                                                                         ccGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTgcgc                                        >  1:42943/1‑149 (MQ=255)
                                                                                                          cGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTgcgc                                        >  8:323160/1‑148 (MQ=255)
                                                                                                              gCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCg                                   >  3:374674/1‑149 (MQ=255)
                                                                                                                       cGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATa                                                                        <  1:11051/103‑1 (MQ=255)
                                                                                                                       cGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATa                                                                        >  2:11051/1‑103 (MQ=255)
                                                                                                                         tCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCggg                        <  4:182525/149‑1 (MQ=255)
                                                                                                                           aGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATg                                                    >  3:340007/1‑119 (MQ=255)
                                                                                                                            gTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCgggg                       >  5:306675/1‑147 (MQ=255)
                                                                                                                             tGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAg                    >  7:43715/1‑149 (MQ=255)
                                                                                                                             tGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAg                    >  7:37660/1‑149 (MQ=255)
                                                                                                                              gggTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAgg                   <  1:6577/149‑1 (MQ=255)
                                                                                                                                gTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGgc                 >  5:334001/1‑149 (MQ=255)
                                                                                                                                 tCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGgcg                >  1:247743/1‑149 (MQ=255)
                                                                                                                                   aTCCGTGGCCTTGTCCAGGCCGGAGTCGACCCATGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCt              >  8:123208/1‑149 (MQ=255)
                                                                                                                                    tCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTc             <  7:231910/149‑1 (MQ=255)
                                                                                                                                     ctccggCCTGGACAAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTc             >  7:218067/5‑148 (MQ=255)
                                                                                                                                     ctccggCCTGGACAAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTc             >  7:212376/5‑148 (MQ=255)
                                                                                                                                     ccGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTc             >  6:340389/1‑148 (MQ=255)
                                                                                                                                     ccGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTc             >  2:218093/1‑148 (MQ=255)
                                                                                                                                       gTGGCCTTGTCCAGGCCGGAGTCGACCCATGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCGCGaa          <  7:123208/149‑1 (MQ=255)
                                                                                                                                       gTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGaa          <  5:170589/149‑1 (MQ=255)
                                                                                                                                        tGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAg         <  2:42943/149‑1 (MQ=255)
                                                                                                                                         ggCCTGGACAAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAgg        <  8:212376/149‑1 (MQ=255)
                                                                                                                                         ggCCTGGACAAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAgg        <  8:218067/149‑1 (MQ=255)
                                                                                                                                            cTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCCTAGGCGGCGCGGGCCAGACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAGgcgc     >  7:248826/1‑149 (MQ=255)
                                                                                                                                               gTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAGGCGCttt  >  1:268633/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCGGCGGCCACGTTGTCGGCAGTGCTGTCGTCGCGGGCGCGGTCGAGGTATTCGCCTGCGCGCTCCAGCAGGGCTTCGGCCGCGTGCAAGCGGACCGCCAGATGGCCGAAGCTTTTCAGCGTCAGTGGGTCATCCGTGGCCTTGTCCAGGCCGGAGTCGACCCACGGACGGCTGCGGGTGCGCACGAAGTGCAGGGCATCTTCATAGGCGGCGCGGGCAATACCGGTGTCGATGGCGGCATGGAGGATCTGCGCCAGCGGGCCGACCGGGGTAGGGCGCTCGAAGGCGCTTT  >  NC_002947/276179‑276470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑