Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,982,835	G→T	7.4%	G118G (GGG→GGT)	PP_3510 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,982,835	0	G	T	7.4%	158.3 / 3.3	54	G118G (GGG→GGT)	PP_3510	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base T (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AAAGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAG  >  NC_002947/3982691‑3982980                                                                                                                                                 |                                                                                                                                                  aaaGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAgggg                                                                                                                                                   >  3:41786/1‑145 (MQ=255) aaaGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAgggg                                                                                                                                                   <  4:41786/145‑1 (MQ=255)  aaGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCAtt                                                                                                                                               >  1:287042/1‑148 (MQ=255)   aGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGa                                                                                                                                             <  7:20738/149‑1 (MQ=255)       aCGTATGCTGGCCCTGCCCTGCCCGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGCCAGCGAAGCGGCGTGTGCACGGGTGCGTGGCGGGGACTGGGGAAAACGCCTGCCGGGGGATTGCCCCt                                                                                                                                         >  4:282996/1‑149 (MQ=255)       aCGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTAAGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCt                                                                                                                                         >  5:339612/1‑149 (MQ=255)         gTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGTGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGc                                                                                                                                       <  2:287042/149‑1 (MQ=255)          tATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      >  7:202669/1‑149 (MQ=255)             gCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcagg                                                                                                                                    <  7:269829/148‑4 (MQ=255)               tGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGCAAAACGCCTGCAGGGGCATTGCCCCTGCGGttt                                                                                                                                  >  8:128969/1‑148 (MQ=255)                 gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGAc                                                                                                                               <  1:300002/149‑1 (MQ=255)                   aCTGACCTGCCAGGCATTGGACCACGCCGGAATTCAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                             >  2:163903/1‑149 (MQ=255)                   aCTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTACATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                             <  1:107417/149‑1 (MQ=255)                     tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                             >  2:18133/1‑147 (MQ=255)                       aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGt                                                                                                                         <  2:113955/149‑1 (MQ=255)                       aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGAGGTTTGACGATAGt                                                                                                                         >  8:161262/1‑149 (MQ=255)                        ccTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTa                                                                                                                        <  2:226118/149‑1 (MQ=255)                         cTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGTGGCATTGACCCTGCGGTTTGACGATAGTAt                                                                                                                       <  7:227583/149‑1 (MQ=255)                             cAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCt                                                                                                                   >  6:144427/1‑149 (MQ=255)                                    tGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      >  2:328962/1‑123 (MQ=255)                                     ggACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCAccc                                                                                                           >  4:231302/1‑149 (MQ=255)                                      gACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      >  8:310988/1‑121 (MQ=255)                                      gACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      <  7:310988/121‑1 (MQ=255)                                                gAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                <  7:83266/149‑1 (MQ=255)                                                       gCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGc                                                                                         <  5:94258/149‑1 (MQ=255)                                                         tGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCg                                                                                       >  8:333103/1‑149 (MQ=255)                                                             ccATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTcc                                                                                   <  8:202669/149‑1 (MQ=255)                                                              cATCCCGCTTGGCATGTACGCTATGACAGCGAAGTGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                >  1:52482/1‑135 (MQ=255)                                                              cATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                <  2:52482/135‑1 (MQ=255)                                                                   cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACt                                                                                            <  5:10818/134‑1 (MQ=255)                                                                   cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACt                                                                                            >  6:10818/1‑134 (MQ=255)                                                                   cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATcc                                                                             <  1:18133/149‑1 (MQ=255)                                                                   cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATcc                                                                             <  5:144427/149‑1 (MQ=255)                                                                    gCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCg                                                                            >  2:310689/1‑149 (MQ=255)                                                                         gCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCaa                                                                        <  3:231302/148‑1 (MQ=255)                                                                           aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCg                                                                                                                >  1:250169/1‑106 (MQ=255)                                                                           aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCg                                                                                                                <  2:250169/106‑1 (MQ=255)                                                                           aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGCGGACTGGGGAAAACGAATGCAGCGGCAATGACCCTGCGGTTTGACGATAGTATCGCTTAGCACCCAATCAATTCGTGACTGGCCGTTACCGATACGCCAAGGa                                                                     >  5:13442/1‑149 (MQ=255)                                                                                 gCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGAATGTAtt                                                               <  6:63162/149‑1 (MQ=255)                                                                                       aCAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCATGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTg                                                         <  6:339612/149‑1 (MQ=255)                                                                                       aCAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTg                                                         <  3:355947/149‑1 (MQ=255)                                                                                          gCGAAGCGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCa                                                      <  3:282996/149‑1 (MQ=255)                                                                                                         cACGGGTGCATGGCGTGGACTTGGGAAAAGGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATaa                                        <  7:128969/148‑1 (MQ=255)                                                                                                          aCGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAca                                      <  7:333103/149‑1 (MQ=255)                                                                                                            gggTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAca                                      <  4:73581/147‑1 (MQ=255)                                                                                                            gggTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAca                                      >  3:73581/1‑147 (MQ=255)                                                                                                                        gggACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGAt                        >  8:120924/1‑149 (MQ=255)                                                                                                                             tGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCggg                    >  5:291041/1‑148 (MQ=255)                                                                                                                               gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGATCGCTCGGGctg                 <  6:13442/149‑3 (MQ=255)                                                                                                                                 gAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACtt               >  3:187050/1‑149 (MQ=255)                                                                                                                                  aaaaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGAACTTg              >  4:199930/1‑149 (MQ=255)                                                                                                                                    tcaaaccgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCgg                     >  7:2993/8‑140 (MQ=255)                                                                                                                                    tcaaaccgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCgg                     <  8:2993/133‑1 (MQ=255)                                                                                                                                     aCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa           <  1:163903/149‑1 (MQ=255)                                                                                                                                       gCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACACCAGCCCGAGCGATCGGGCACTTGCAAGa         <  1:217030/149‑1 (MQ=255)                                                                                                                                        accgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGa         >  4:340217/4‑148 (MQ=255)                                                                                                                                              gtggCATTGACCCTGAGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAg  <  7:161262/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AAAGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAG  >  NC_002947/3982691‑3982980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑