Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,982,841	A→C	10.1%	*120C (TGA→TGC)	PP_3510 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,982,841	0	A	C	10.1%	154.0 / 5.4	55	*120C (TGA→TGC)	PP_3510	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/28);  new base C (4/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAG  >  NC_002947/3982693‑3982980                                                                                                                                                     |                                                                                                                                            aGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGa                                                                                                                                             <  7:20738/149‑1 (MQ=255)     aCGTATGCTGGCCCTGCCCTGCCCGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGCCAGCGAAGCGGCGTGTGCACGGGTGCGTGGCGGGGACTGGGGAAAACGCCTGCCGGGGGATTGCCCCt                                                                                                                                         >  4:282996/1‑149 (MQ=255)     aCGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTAAGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCt                                                                                                                                         >  5:339612/1‑149 (MQ=255)       gTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGTGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGc                                                                                                                                       <  2:287042/149‑1 (MQ=255)        tATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      >  7:202669/1‑149 (MQ=255)           gCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGTCAATGCCCCTGcagg                                                                                                                                    <  7:269829/148‑4 (MQ=255)             tGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGCAAAACGCCTGCAGGGGCATTGCCCCTGCGGttt                                                                                                                                  >  8:128969/1‑148 (MQ=255)               gCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGAc                                                                                                                               <  1:300002/149‑1 (MQ=255)                 aCTGACCTGCCAGGCATTGGACCACGCCGGAATTCAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                             >  2:163903/1‑149 (MQ=255)                 aCTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTACATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                             <  1:107417/149‑1 (MQ=255)                   tGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGa                                                                                                                             >  2:18133/1‑147 (MQ=255)                     aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGt                                                                                                                         <  2:113955/149‑1 (MQ=255)                     aCCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGAGGTTTGACGATAGt                                                                                                                         >  8:161262/1‑149 (MQ=255)                      ccTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTa                                                                                                                        <  2:226118/149‑1 (MQ=255)                       cTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGTGGCATTGACCCTGCGGTTTGACGATAGTAt                                                                                                                       <  7:227583/149‑1 (MQ=255)                           cAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCt                                                                                                                   >  6:144427/1‑149 (MQ=255)                                  tGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      >  2:328962/1‑123 (MQ=255)                                   ggACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCAccc                                                                                                           >  4:231302/1‑149 (MQ=255)                                    gACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      <  7:310988/121‑1 (MQ=255)                                    gACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCg                                                                                                                                      >  8:310988/1‑121 (MQ=255)                                              gAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                <  7:83266/149‑1 (MQ=255)                                                     gCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGc                                                                                         <  5:94258/149‑1 (MQ=255)                                                       tGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCg                                                                                       >  8:333103/1‑149 (MQ=255)                                                           ccATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTcc                                                                                   <  8:202669/149‑1 (MQ=255)                                                            cATCCCGCTTGGCATGTACGCTATGACAGCGAAGTGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                >  1:52482/1‑135 (MQ=255)                                                            cATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGt                                                                                                <  2:52482/135‑1 (MQ=255)                                                                 cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACt                                                                                            >  6:10818/1‑134 (MQ=255)                                                                 cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACt                                                                                            <  5:10818/134‑1 (MQ=255)                                                                 cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATcc                                                                             <  5:144427/149‑1 (MQ=255)                                                                 cGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATcc                                                                             <  1:18133/149‑1 (MQ=255)                                                                  gCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCg                                                                            >  2:310689/1‑149 (MQ=255)                                                                       gCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCaa                                                                        <  3:231302/148‑1 (MQ=255)                                                                         aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCg                                                                                                                >  1:250169/1‑106 (MQ=255)                                                                         aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCg                                                                                                                <  2:250169/106‑1 (MQ=255)                                                                         aTGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGCGGACTGGGGAAAACGAATGCAGCGGCAATGACCCTGCGGTTTGACGATAGTATCGCTTAGCACCCAATCAATTCGTGACTGGCCGTTACCGATACGCCAAGGa                                                                     >  5:13442/1‑149 (MQ=255)                                                                               gCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGAATGTAtt                                                               <  6:63162/149‑1 (MQ=255)                                                                                     aCAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCATGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTg                                                         <  6:339612/149‑1 (MQ=255)                                                                                     aCAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTg                                                         <  3:355947/149‑1 (MQ=255)                                                                                        gCGAAGCGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCa                                                      <  3:282996/149‑1 (MQ=255)                                                                                                       cACGGGTGCATGGCGTGGACTTGGGAAAAGGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATaa                                        <  7:128969/148‑1 (MQ=255)                                                                                                        aCGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAca                                      <  7:333103/149‑1 (MQ=255)                                                                                                          gggTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAca                                      <  4:73581/147‑1 (MQ=255)                                                                                                          gggTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAAca                                      >  3:73581/1‑147 (MQ=255)                                                                                                                      gggACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGAt                        >  8:120924/1‑149 (MQ=255)                                                                                                                           tGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCggg                    >  5:291041/1‑148 (MQ=255)                                                                                                                             gggAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGATCGCTCGGGctg                 <  6:13442/149‑3 (MQ=255)                                                                                                                               gAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACtt               >  3:187050/1‑149 (MQ=255)                                                                                                                                aaaaCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGAACTTg              >  4:199930/1‑149 (MQ=255)                                                                                                                                  tcaaaccgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCgg                     >  7:2993/8‑140 (MQ=255)                                                                                                                                  tcaaaccgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCgg                     <  8:2993/133‑1 (MQ=255)                                                                                                                                   aCGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCaa           <  1:163903/149‑1 (MQ=255)                                                                                                                                     gCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACACCAGCCCGAGCGATCGGGCACTTGCAAGa         <  1:217030/149‑1 (MQ=255)                                                                                                                                      accgCAGGGTCAATGCCCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGa         >  4:340217/4‑148 (MQ=255)                                                                                                                                            gtggCATTGACCCTGAGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAg  <  7:161262/147‑1 (MQ=255)                                                                                                                                                 aTTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCatat                                          >  6:181861/1‑104 (MQ=255)                                                                                                                                                 aTTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCatat                                          <  5:181861/104‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            AGGCACGTATGCTGGCACTGACCTGCCAGGCATTGGACCACGCCGGAATACAGCTGGACCATCCCGCTTGGCATGTACGCTATGACAGCGAAGAGGCCTGTGCACGGGTGCATGGCGGGGACTGGGGAAAACGCCTGCAGGGGCATTGACCCTGCGGTTTGACGATAGTATCGCTTCGCACCCTATCAATTCGTGACTGGCCGTTCCCGATCCGCCAAGGATGTATTAAGGTGGCATGGTGCGCATATAACAGCAGCCCGAGCGATCGGGCACTTGCAAGACAATGAG  >  NC_002947/3982693‑3982980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑