Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,007,812 C→T 7.2% A22T (GCC→ACC)  PP_3535 ← gamma‑glutamyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,007,8120CT 7.2% 162.9 / 2.1 55A22T (GCC→ACC) PP_3535gamma‑glutamyltransferase
Reads supporting (aligned to +/- strand):  ref base C (21/30);  new base T (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GCCGCCGGCACGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCTGACTTCAAGCCTTTGC  >  NC_002947/4007669‑4007947
                                                                                                                                               |                                                                                                                                       
gccgccGGCACGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGCCCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCaccga                                                                                                                                    >  7:182123/1‑149 (MQ=255)
 ccgccgGCACGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCaccga                                                                                                                                    <  8:246883/148‑1 (MQ=255)
     cgGCACGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAg                                                                                                                               >  7:329862/1‑149 (MQ=255)
        cACGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCg                                                                                                                            <  3:109257/149‑1 (MQ=255)
         aCGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGc                                                                                                                           >  3:13915/1‑149 (MQ=255)
           gCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGTgccgaccag                                                                                                                                <  4:344216/142‑3 (MQ=255)
           gCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGTgccgaccag                                                                                                                                >  3:344216/1‑140 (MQ=255)
            cAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGa                                                                                                                        >  5:59034/1‑149 (MQ=255)
             aaCATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGAt                                                                                                                       >  7:192689/1‑149 (MQ=255)
             aaCATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGAt                                                                                                                       >  3:272155/1‑149 (MQ=255)
                aTCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGTgccgaccag                                                                                                                                >  7:169502/1‑135 (MQ=255)
                aTCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGTgccgaccag                                                                                                                                <  8:169502/137‑3 (MQ=255)
                aTCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGc                                                                                                                    <  6:285011/149‑1 (MQ=255)
                      cgccccgcTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAg                                                                                                              >  1:273176/1‑149 (MQ=255)
                              ttCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCCCAGCg                                                                                                      >  2:111682/1‑149 (MQ=255)
                                 aCTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTc                                                                                                   <  7:229357/149‑1 (MQ=255)
                                  cTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCt                                                                                                  <  1:162712/149‑1 (MQ=255)
                                     gCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTg                                                                                                 <  8:292188/147‑1 (MQ=255)
                                     gCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTg                                                                                                 <  4:142933/147‑1 (MQ=255)
                                     gCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGAt                                                                                               <  2:106349/149‑1 (MQ=255)
                                      ccAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGAt                                                                                               >  3:55401/1‑148 (MQ=255)
                                          cGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTg                                                                                          <  2:292198/149‑1 (MQ=255)
                                            ggTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTggg                                                                                        <  1:61830/149‑1 (MQ=255)
                                             gTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGtt                                                                                                    <  3:351626/136‑1 (MQ=255)
                                             gTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGtt                                                                                                    >  4:351626/1‑136 (MQ=255)
                                                           ccATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATc                                                                         <  4:13915/149‑1 (MQ=255)
                                                           ccATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATc                                                                         <  4:8104/149‑1 (MQ=255)
                                                            cATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCg                                                                        <  6:59034/149‑1 (MQ=255)
                                                             aTGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCgc                                                                       <  1:304130/149‑1 (MQ=255)
                                                             aTGTACCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCAtt                                                                                                                <  6:308569/108‑1 (MQ=255)
                                                             aTGTACCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCAtt                                                                                                                >  5:308569/1‑108 (MQ=255)
                                                                      cTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCt                                                              <  8:329862/149‑1 (MQ=255)
                                                                              cACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCctctct                                                      >  3:190014/1‑149 (MQ=255)
                                                                               aCCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCtt                                                     <  5:121578/149‑1 (MQ=255)
                                                                                ccGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCttt                                                    <  2:273176/149‑1 (MQ=255)
                                                                                  ggCTGCAAACCGCTGCGGTAGCCGGAGGCCTGGTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGACCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTAt                                                  <  8:322418/149‑1 (MQ=255)
                                                                                         aaCCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCct                                                          <  4:359882/134‑1 (MQ=255)
                                                                                         aaCCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCct                                                          >  3:359882/1‑134 (MQ=255)
                                                                                          aCCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAgg                                          <  4:190014/149‑1 (MQ=255)
                                                                                          aCCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAgg                                          <  8:132504/149‑1 (MQ=255)
                                                                                          aCCGCGGCGGGAGCCGGCGGCCAGTTCCGGGGGCGTTGGCAAAACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTACGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAgg                                          <  8:243000/149‑1 (MQ=255)
                                                                                              cTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTc                                                                                                   >  6:391896/1‑88 (MQ=255)
                                                                                                   gTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACAGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTgg                                 >  6:387204/1‑149 (MQ=255)
                                                                                                      gCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGc                               <  4:55401/148‑1 (MQ=255)
                                                                                                               cAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGt                        <  5:391540/146‑1 (MQ=255)
                                                                                                               cAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGt                        >  6:391540/1‑146 (MQ=255)
                                                                                                                  ttCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCt                  >  5:284470/1‑149 (MQ=255)
                                                                                                                     cGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCTGa                >  3:328262/1‑148 (MQ=255)
                                                                                                                     cGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCTGa                <  4:328262/148‑1 (MQ=255)
                                                                                                                          gCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCTGACTTc            >  3:190455/1‑147 (MQ=255)
                                                                                                                                 cAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTg                                                                                          <  3:57835/62‑1 (MQ=255)
                                                                                                                                 cAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTg                                                                                          >  4:57835/1‑62 (MQ=255)
                                                                                                                                  aaTACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCt                  <  7:303257/133‑1 (MQ=255)
                                                                                                                                  aaTACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCt                  >  8:303257/1‑133 (MQ=255)
                                                                                                                                  aaTACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCTGACTTCAAGCCTTTgc  <  8:192689/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                       
GCCGCCGGCACGCAACATCGCCCGCCCCGCTTCACTGGCCAGCGGGTTGGCTGCGGCGACCATGTGCCGGCTGGCGTGCACCGGCTGCAAACCGCTGCGGTAGCCGGAGGCCAGTTCCGGTGGCGTGGGCAATACCTGGTCGGCACCGACCGAGCCGCTGATGGCCATTAGGCACAGCGTTCTGATCAGTGGGATAGAGAACTTCATCGCTTGCTTCCTGCCTCTCTTTATTCAGCAGGGCACTGTGGGCCAGGAGTGGACCTGACTTCAAGCCTTTGC  >  NC_002947/4007669‑4007947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑