Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,061,390 C→A 8.3% L107M (CTG→ATG) ‡ PP_3579 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,061,3900CA 8.3% 183.5 / 6.7 60L107M (CTG→ATG) ‡PP_3579membrane protein
Reads supporting (aligned to +/- strand):  ref base C (27/28);  new base A (2/3);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

GCCAGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGCGAGC  >  NC_002947/4061243‑4061531
                                                                                                                                                   |                                                                                                                                             
gccaGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCt                                                                                                                                              <  6:327334‑M2/145‑1 (MQ=255)
 ccaGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTg                                                                                                                                             >  4:94524‑M2/4‑149 (MQ=255)
 ccaGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTg                                                                                                                                             >  3:4013‑M2/4‑149 (MQ=255)
 ccaGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTg                                                                                                                                             >  7:47499‑M2/4‑149 (MQ=255)
   aGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAAGTGGTCTCCCGCCGCGGCCTGCACGGCACGGCCCTGGc                                                                                                                                           >  8:302050‑M2/2‑149 (MQ=255)
   aGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGc                                                                                                                                           <  6:104963‑M2/148‑1 (MQ=255)
    gccCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGcc                                                                                                                                          >  5:230526/1‑149 (MQ=255)
    gccCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGcc                                                                                                                                          <  1:162830/149‑1 (MQ=255)
       cGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCAGGGCCGTg                                                                                                                                       <  4:315569/149‑1 (MQ=255)
        ggAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGc                                                                                                                                      <  3:171776/149‑1 (MQ=255)
         gAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGACGCGGCCTGCAAGGCACGGCCAGGGCCGTGcc                                                                                                                                     <  3:259888/149‑2 (MQ=255)
          aaaaGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCa                                                                                                                                     >  5:44304/1‑148 (MQ=255)
           aaaGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCAGGGCCGTGcctt                                                                                                                                   <  7:108126/149‑4 (MQ=255)
             aGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTAt                                                                                                                                 <  2:150786/149‑1 (MQ=255)
               gTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTAtct                                                                                                                               <  2:216775/149‑1 (MQ=255)
                 ccGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATctct                                                                                                                             <  7:302050/149‑1 (MQ=255)
                   gCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTac                                                                                                                           <  3:303522/149‑1 (MQ=255)
                         tCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGAACGTGTGCATCGCCTCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGGCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGc                                                                                                                     <  7:124741/149‑1 (MQ=255)
                                   cGAAGAAGAGAAAGCCGGACTGGTCCTGCTGACCTTGTGCATCGCCTCCATCGCCAGCCTTGCCGCGGTCACCCTGAAACTGGTCTCCAGCCGCGGCCTGCAAGGAACGGCCCTGGCCGTGCACTATCTATACACTGGCATGACCGtag                                                                                                           >  4:101952/1‑147 (MQ=255)
                                     aaGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGcc                                                                                                         <  7:153536/149‑1 (MQ=255)
                                            gaACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCt                                                                                                  >  2:278396/1‑149 (MQ=255)
                                                  cGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGgctgc                                                                                            <  2:24731/149‑1 (MQ=255)
                                                    gCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGctgctg                                                                                          <  2:314961/149‑1 (MQ=255)
                                                      cTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTgg                                                                                                <  5:237555/141‑1 (MQ=255)
                                                      cTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTgg                                                                                                >  6:237555/1‑141 (MQ=255)
                                                            ctgctgACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCt                                                                                   <  6:44304/148‑1 (MQ=255)
                                                            ctgctgACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCt                                                                                   <  1:278396/148‑1 (MQ=255)
                                                            ctgctgACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTg                                                                                  <  8:312585/149‑1 (MQ=255)
                                                              gctgACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCa                                                                                >  7:13653/1‑149 (MQ=255)
                                                                  aCCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCata                                                                              <  3:94524/147‑1 (MQ=255)
                                                                          cATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTg                                                                     <  8:47499/148‑1 (MQ=255)
                                                                          cATCGCCGCCATCGCCAGCCTTGCCGCGGTAACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGACGTGCACTATCTCTACACTGGCATGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGc                                                                    <  3:101952/149‑1 (MQ=255)
                                                                           aTCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCAcc                                                                        <  7:210757/144‑1 (MQ=255)
                                                                           aTCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCAcc                                                                        >  8:210757/1‑144 (MQ=255)
                                                                              gccgccATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCTGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTa                                                                >  8:321876/1‑149 (MQ=255)
                                                                                cgccATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCa                                                                   >  3:145798/1‑144 (MQ=255)
                                                                                cgccATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCa                                                                   <  4:145798/144‑1 (MQ=255)
                                                                                       gCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCt                                                       >  4:317885/1‑149 (MQ=255)
                                                                                        ccAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTAcgcccggtgt                                                      >  7:44780/1‑145 (MQ=255)
                                                                                                 ccGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACAcc                                             >  2:64678/1‑149 (MQ=255)
                                                                                                         aCCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCaaaa                                     <  6:230526/149‑1 (MQ=255)
                                                                                                           ccTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCCGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAAc                                    >  1:50059/1‑148 (MQ=255)
                                                                                                               cAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCAc                                 <  3:317885/147‑1 (MQ=255)
                                                                                                                aaCTGGTCTCCAGCAGAGGCCTGCAAGTCACGGCCCTGGCCGTGAAATATATCTACACTGTCCTGACAGTCGCCGGTTCCTGGCTGCTGATAGGCTGCATAATCAACCTGAAATACCCACGCCGGTTCTACACCAGGCAAAAcaccga                               >  6:254974/1‑143 (MQ=255)
                                                                                                                  cTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAAcc                            >  5:26077/1‑149 (MQ=255)
                                                                                                                     gTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAAc                             >  4:136454/1‑145 (MQ=255)
                                                                                                                     gTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAAc                             <  3:136454/145‑1 (MQ=255)
                                                                                                                          cAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCAc                       <  7:321876/146‑1 (MQ=255)
                                                                                                                                    cTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGCTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCGGa          >  7:86660/1‑149 (MQ=255)
                                                                                                                                      gCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATg        >  6:328690/1‑149 (MQ=255)
                                                                                                                                       cAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATgg       >  1:382844/1‑149 (MQ=255)
                                                                                                                                        agtgCACGGCCAGGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGc      >  8:246264/4‑149 (MQ=255)
                                                                                                                                        aaGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGc      <  8:13653/149‑1 (MQ=255)
                                                                                                                                         aGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGc             <  8:123274/141‑1 (MQ=255)
                                                                                                                                         aGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGc             >  7:123274/1‑141 (MQ=255)
                                                                                                                                          tgCACGGCCAGGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTACACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGc      >  7:310888/2‑147 (MQ=255)
                                                                                                                                            cACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGCGAgc  >  6:36302/1‑149 (MQ=255)
                                                                                                                                            cACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGCGAgc  >  4:352849/1‑149 (MQ=255)
                                                                                                                                            cACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGCGAgc  <  4:4013/149‑1 (MQ=255)
                                                                                                                                              cGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGTGaac  >  2:111432/1‑145 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GCCAGCCCGGAAAAGGTCCGCAAGGTCGCCGGCGTCGAAGACGAGAACGCCGGCCTGGTCCTGCTGACCGTGTGCATCGCCGCCATCGCCAGCCTTGCCGCGGTCACCCTGCAACTGGTCTCCAGCCGCGGCCTGCAAGGCACGGCCCTGGCCGTGCACTATCTCTACACTGGCCTGACCGTCGCCGGTTCCTGGCTGCTGATCGGCTGCATATTCACCCTGCACTACGCCCGCCTGTTCTACACCGGGCAAAACCACGAACCCCCACTGCGCTTCGCTGATGGCGAGC  >  NC_002947/4061243‑4061531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑