Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,062,312	G→A	9.2%	P676L (CCG→CTG)	PP_3581 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,062,312	0	G	A	9.2%	148.5 / 5.0	54	P676L (CCG→CTG)	PP_3581	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base A (2/3);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

CCGCCGCCGATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCT  >  NC_002947/4062166‑4062458                                                                                                                                                   |                                                                                                                                                   ccgccgccgATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTcggccgg                                                                                                                                                   >  3:318023/1‑148 (MQ=255)      gccgATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACggg                                                                                                                                             <  7:109130/149‑1 (MQ=255)         gaggttgagCGCATCATCGCGGCCGTGGAGGCGGTAATGTCTGAGTTCATACCCGCCTTTACCTTGCGCGATCCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                          <  6:309428/143‑1 (MQ=255)         gATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                          <  7:19999/149‑1 (MQ=255)         gATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                          >  3:204464/1‑149 (MQ=255)         gATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                          >  3:239870/1‑149 (MQ=255)          aTGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTgg                                                                                                                                         <  2:183696/149‑1 (MQ=255)               gagCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCca                                                                                                                                     <  3:227548/148‑1 (MQ=255)                agCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTGGGCCAGCCCGTGCCGGCCgagc                                                                                                                                   <  2:225974/149‑1 (MQ=255)                     atcatcGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTACAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAgg                                                                                                                                >  8:301422/1‑147 (MQ=255)                        atcGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTGGGCCAGCCCGTGCCGGCCgagcagttcat                                                                                                                            <  1:282778/148‑6 (MQ=38)                           gAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCt                                                                                                                         <  7:178496/148‑1 (MQ=255)                                cGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAAc                                                                                                                    >  7:21438/1‑148 (MQ=255)                                cGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAAc                                                                                                                    >  8:130228/1‑148 (MQ=255)                                 gTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGa                                                                                                                  <  4:239870/149‑1 (MQ=255)                                 gTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGa                                                                                                                  <  4:204464/149‑1 (MQ=255)                                    gAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGt                                                                                                                <  1:344172/148‑1 (MQ=255)                                         ggAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCAGCCCGTGCCGGCCgag                                                                                                                                    <  5:219033/123‑1 (MQ=255)                                         ggAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCAGCCCGTGCCGGCCgag                                                                                                                                    >  6:219033/1‑123 (MQ=255)                                                 aTGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACgg                                                                                                  >  7:36863/1‑149 (MQ=255)                                                          tACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAg                                                                                         <  1:271312/149‑1 (MQ=255)                                                             ccGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTcc                                                                                      >  6:144204/1‑149 (MQ=255)                                                             ccGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTcc                                                                                      >  6:10214/1‑149 (MQ=255)                                                              cGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCct                                                                                     <  4:92703/149‑1 (MQ=255)                                                                ccTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTACAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCctgc                                                                                   <  7:301422/149‑1 (MQ=255)                                                                       cTTGCGCGATGAAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCCCATCCCAGACGGGTCAGGAAGTCGTGCTGCTTGt                                                                            >  8:343451/1‑149 (MQ=255)                                                                            gcgATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGtt                                                                       <  8:36863/149‑1 (MQ=255)                                                                                   aGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAccc                                                                >  2:175676/1‑149 (MQ=255)                                                                                     tCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAcccc                                                               >  6:33491/1‑148 (MQ=255)                                                                                       tAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCACCCCCCTc                                                            >  4:349833/1‑149 (MQ=255)                                                                                        aGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCg                                                           >  4:281383/1‑149 (MQ=255)                                                                                          cccGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAgg                                                                                            <  6:227467/114‑1 (MQ=255)                                                                                          cccGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAgg                                                                                            >  5:227467/1‑114 (MQ=255)                                                                                            cGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCaa                                                       >  5:145332/1‑149 (MQ=255)                                                                                                 cagTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATa                                                  >  1:277930/1‑149 (MQ=255)                                                                                                    tGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACg                                                >  4:103344/1‑148 (MQ=255)                                                                                                    tGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGc                                               <  3:103344/149‑1 (MQ=255)                                                                                                        ttCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGc                                           <  5:33491/149‑1 (MQ=255)                                                                                                          ccGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGcc                                          >  5:33870/1‑148 (MQ=255)                                                                                                             ggCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGGTTCGACCCCCTCGGCAACGATCCGCCGGCCCAAc                                      >  8:338603/1‑149 (MQ=255)                                                                                                                ccATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCaa                                                       <  5:283778/129‑1 (MQ=255)                                                                                                                ccATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCaa                                                       >  6:283778/1‑129 (MQ=255)                                                                                                                 cATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCt                                  >  5:175039/1‑149 (MQ=255)                                                                                                                     gCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGcc                              <  8:21438/149‑1 (MQ=255)                                                                                                                             cTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGcga                      <  7:130228/149‑1 (MQ=255)                                                                                                                              ttGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAAAGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGcgac                     >  4:75390/1‑149 (MQ=255)                                                                                                                                gCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGcgacga                   >  3:96617/1‑149 (MQ=255)                                                                                                                                  tCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGAtt                 >  1:76396/1‑149 (MQ=255)                                                                                                                                    aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAc                                                                  >  4:119258/1‑98 (MQ=255)                                                                                                                                    aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAc                                                                  <  3:119258/98‑1 (MQ=255)                                                                                                                                    aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGc               <  6:175039/149‑1 (MQ=255)                                                                                                                                    aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTCCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGAAGATTGc               >  5:163001/1‑149 (MQ=255)                                                                                                                                         ctgctgGGCCAGCCCGTGCCGGCCGAGCAGGTAGCCCTGCAACGAGTCACATCCCAGCCGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGaaa          >  5:313091/1‑149 (MQ=255)                                                                                                                                         cTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGaaa          <  3:75390/149‑1 (MQ=255)                                                                                                                                            cTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGaaaaaa       >  8:341367/1‑149 (MQ=255)                                                                                                                                                gccggcACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGc   <  5:144204/149‑1 (MQ=255)                                                                                                                                                 ccggcACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCt  >  4:76406/1‑149 (MQ=255)                                                                                                                                                 ccggcACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCt  <  2:277930/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCGCCGCCGATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCT  >  NC_002947/4062166‑4062458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑