Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,062,318 G→T 7.8% P674H (CCC→CAC)  PP_3581 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,062,3180GT 7.8% 179.1 / 2.3 58P674H (CCC→CAC) PP_3581membrane protein
Reads supporting (aligned to +/- strand):  ref base G (30/23);  new base T (2/3);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCCGATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCTGCATCGT  >  NC_002947/4062171‑4062465
                                                                                                                                                   |                                                                                                                                                   
gccgATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACggg                                                                                                                                                    <  7:109130/149‑1 (MQ=255)
   gaggttgagCGCATCATCGCGGCCGTGGAGGCGGTAATGTCTGAGTTCATACCCGCCTTTACCTTGCGCGATCCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                                 <  6:309428/143‑1 (MQ=255)
   gATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                                 <  7:19999/149‑1 (MQ=255)
   gATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                                 >  3:204464/1‑149 (MQ=255)
   gATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTg                                                                                                                                                 >  3:239870/1‑149 (MQ=255)
    aTGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTgg                                                                                                                                                <  2:183696/149‑1 (MQ=255)
         gagCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCca                                                                                                                                            <  3:227548/148‑1 (MQ=255)
          agCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTGGGCCAGCCCGTGCCGGCCgagc                                                                                                                                          <  2:225974/149‑1 (MQ=255)
               atcatcGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTACAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAgg                                                                                                                                       >  8:301422/1‑147 (MQ=255)
                  atcGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTGGGCCAGCCCGTGCCGGCCgagcagttcat                                                                                                                                   <  1:282778/148‑6 (MQ=38)
                     gAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCt                                                                                                                                <  7:178496/148‑1 (MQ=255)
                          cGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAAc                                                                                                                           >  8:130228/1‑148 (MQ=255)
                          cGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAAc                                                                                                                           >  7:21438/1‑148 (MQ=255)
                           gTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGa                                                                                                                         <  4:239870/149‑1 (MQ=255)
                           gTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGa                                                                                                                         <  4:204464/149‑1 (MQ=255)
                              gAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGt                                                                                                                       <  1:344172/148‑1 (MQ=255)
                                   ggAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCAGCCCGTGCCGGCCgag                                                                                                                                           >  6:219033/1‑123 (MQ=255)
                                   ggAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCAGCCCGTGCCGGCCgag                                                                                                                                           <  5:219033/123‑1 (MQ=255)
                                           aTGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACgg                                                                                                         >  7:36863/1‑149 (MQ=255)
                                                    tACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAg                                                                                                <  1:271312/149‑1 (MQ=255)
                                                       ccGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTcc                                                                                             >  6:144204/1‑149 (MQ=255)
                                                       ccGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTcc                                                                                             >  6:10214/1‑149 (MQ=255)
                                                        cGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCct                                                                                            <  4:92703/149‑1 (MQ=255)
                                                          ccTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTACAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCctgc                                                                                          <  7:301422/149‑1 (MQ=255)
                                                                 cTTGCGCGATGAAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCCCATCCCAGACGGGTCAGGAAGTCGTGCTGCTTGt                                                                                   >  8:343451/1‑149 (MQ=255)
                                                                      gcgATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGtt                                                                              <  8:36863/149‑1 (MQ=255)
                                                                             aGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAccc                                                                       >  2:175676/1‑149 (MQ=255)
                                                                               tCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAcccc                                                                      >  6:33491/1‑148 (MQ=255)
                                                                                 tAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCACCCCCCTc                                                                   >  4:349833/1‑149 (MQ=255)
                                                                                  aGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCg                                                                  >  4:281383/1‑149 (MQ=255)
                                                                                    cccGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAgg                                                                                                   <  6:227467/114‑1 (MQ=255)
                                                                                    cccGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAgg                                                                                                   >  5:227467/1‑114 (MQ=255)
                                                                                      cGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCaa                                                              >  5:145332/1‑149 (MQ=255)
                                                                                           cagTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATa                                                         >  1:277930/1‑149 (MQ=255)
                                                                                              tGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACg                                                       >  4:103344/1‑148 (MQ=255)
                                                                                              tGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGc                                                      <  3:103344/149‑1 (MQ=255)
                                                                                                  ttCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGc                                                  <  5:33491/149‑1 (MQ=255)
                                                                                                    ccGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGcc                                                 >  5:33870/1‑148 (MQ=255)
                                                                                                       ggCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGGTTCGACCCCCTCGGCAACGATCCGCCGGCCCAAc                                             >  8:338603/1‑149 (MQ=255)
                                                                                                          ccATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCaa                                                              <  5:283778/129‑1 (MQ=255)
                                                                                                          ccATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCaa                                                              >  6:283778/1‑129 (MQ=255)
                                                                                                           cATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCt                                         >  5:175039/1‑149 (MQ=255)
                                                                                                               gCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGcc                                     <  8:21438/149‑1 (MQ=255)
                                                                                                                       cTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGcga                             <  7:130228/149‑1 (MQ=255)
                                                                                                                        ttGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAAAGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGcgac                            >  4:75390/1‑149 (MQ=255)
                                                                                                                          gCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGcgacga                          >  3:96617/1‑149 (MQ=255)
                                                                                                                            tCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGAtt                        >  1:76396/1‑149 (MQ=255)
                                                                                                                              aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAc                                                                         >  4:119258/1‑98 (MQ=255)
                                                                                                                              aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAAc                                                                         <  3:119258/98‑1 (MQ=255)
                                                                                                                              aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGc                      <  6:175039/149‑1 (MQ=255)
                                                                                                                              aTGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTCCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGAAGATTGc                      >  5:163001/1‑149 (MQ=255)
                                                                                                                                   ctgctgGGCCAGCCCGTGCCGGCCGAGCAGGTAGCCCTGCAACGAGTCACATCCCAGCCGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGaaa                 >  5:313091/1‑149 (MQ=255)
                                                                                                                                   cTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGaaa                 <  3:75390/149‑1 (MQ=255)
                                                                                                                                      cTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGaaaaaa              >  8:341367/1‑149 (MQ=255)
                                                                                                                                          gccggcACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGc          <  5:144204/149‑1 (MQ=255)
                                                                                                                                           ccggcACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCt         <  2:277930/149‑1 (MQ=255)
                                                                                                                                           ccggcACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCt         >  4:76406/1‑149 (MQ=255)
                                                                                                                                               cACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCTGCAt     >  2:71281/1‑149 (MQ=255)
                                                                                                                                               cACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCACTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCTgact     >  8:282571/1‑146 (MQ=255)
                                                                                                                                                  gggCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCTGCATCGt  <  6:33870/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCCGATGTAGAGCGCATCATCGAGGCCGTCGAGGCGGAAATGGATGCCTTCATACCCGCCTTTACCTTGCGCGATGCAGTCTAGCCCGCAGCAGTGAGTTCCGGGCCCATGGCCTGCAACTTGCTCATGAACTGCTCGGCCGGCACGGGCTGGCCCAGCAGGTAGCCCTGCAACGAGTCACATCCCAGACGGGTCAGGAAGTCCTGCTGCTTGTCGGTTTCAACCCCCTCGGCAACGATACGCAGGCCCAACGCCTGGCCCAGTGCGACGATTGCCGAAACAATCGCTGCATCGT  >  NC_002947/4062171‑4062465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑