Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,133,421 A→T 7.7% L183Q (CTG→CAG)  PP_3637 ← sulfonate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,133,4210AT 7.7% 149.3 / 3.0 52L183Q (CTG→CAG) PP_3637sulfonate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CGTGGTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGG  >  NC_002947/4133274‑4133567
                                                                                                                                                   |                                                                                                                                                  
cGTGGTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGGGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTgcag                                                                                                                                                   <  6:487/149‑1 (MQ=255)
    gTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTgcagcag                                                                                                                                                >  1:43488/1‑148 (MQ=255)
    gTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTgcagcag                                                                                                                                                >  6:331167/1‑148 (MQ=255)
       gctgCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCaa                                                                                                                                             <  8:83942/145‑1 (MQ=255)
       gCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCaa                                                                                                                                             <  1:336747/148‑1 (MQ=255)
          gCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGTCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAActgc                                                                                                                                         <  2:21115/149‑1 (MQ=255)
             ggTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACtgctgc                                                                                                                                      <  4:355266/149‑1 (MQ=255)
                   tatGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCTGCTGCAActga                                                                                                                                         <  5:150882/140‑2 (MQ=255)
                   tatGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCTGCTGCAActga                                                                                                                                         >  6:150882/1‑139 (MQ=255)
                      gCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGAGATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGgcg                                                                                                                             >  8:186969/1‑149 (MQ=255)
                                gAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCAccg                                                                                                                   >  4:228813/1‑148 (MQ=255)
                                gAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCAc                                                                                                                     <  1:46159/147‑1 (MQ=255)
                                gAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCAc                                                                                                                     >  2:46159/1‑147 (MQ=255)
                                    aTGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATc                                                                                                               <  6:365454/149‑1 (MQ=255)
                                                   ttGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAggg                                                                                                <  3:269335/149‑1 (MQ=255)
                                                       ccAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCa                                                                                            >  3:115409/1‑149 (MQ=255)
                                                       ccAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCa                                                                                            >  6:78616/1‑149 (MQ=255)
                                                        cAGTAGCAGAACCCGGTCAGACAGCACCAGGGCTACGTCGATATCCTCAGTGATCAACACGGCCGCGGTCTTGTGCGAGGCGAACAGTTTCAGCAGCAACTGCTGCATCTCGGCGCGGCTCACCACATCCAACGCACCGCACGGTTCaa                                                                                           >  7:321233/1‑148 (MQ=255)
                                                         aGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATc                                                                                          <  4:203199/149‑1 (MQ=255)
                                                           tAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATc                                                                                          >  4:273896/1‑147 (MQ=255)
                                                                gAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGACTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGcaac                                                                                   <  5:331167/149‑1 (MQ=255)
                                                                 aaCCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGcaac                                                                                   >  5:272266/1‑148 (MQ=255)
                                                                  aCCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGcaacaa                                                                                 <  2:308820/149‑1 (MQ=255)
                                                                       gTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACtt                                                                            >  5:320253/1‑149 (MQ=255)
                                                                        tCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTc                                                                           <  2:234860/149‑1 (MQ=255)
                                                                         cTGACAGCAGCAGGGCTTCGTCGATATCGAGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCAAGTCGGCGCGGGTCACCACATCCAACGCAGCGAAGGGTTCATCCAGcaacaaccgttcg                                                                          >  2:386497/1‑142 (MQ=255)
                                                                            aCAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCgg                                                                         <  5:281083/147‑1 (MQ=255)
                                                                               gcagccgGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTgg                                                                    <  8:60535/149‑1 (MQ=255)
                                                                                   cagGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTg                                                                <  6:386033/149‑1 (MQ=255)
                                                                                     gggCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGcc                                                              <  4:115409/149‑1 (MQ=255)
                                                                                           cgtcgATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGCCTTCGGGCTGGCGTGCCAGGCAg                                                        >  6:48934/1‑149 (MQ=255)
                                                                                             tcgATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCg                                                      <  7:90214/149‑1 (MQ=255)
                                                                                                           gaTCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgc                                        >  7:250869/1‑149 (MQ=255)
                                                                                                             tCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcgc                                      >  4:224650/1‑149 (MQ=255)
                                                                                                              cAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCaa                                                                                                            <  5:374863/78‑1 (MQ=255)
                                                                                                              cAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCaa                                                                                                            >  6:374863/1‑78 (MQ=255)
                                                                                                               aaCACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGGCGGCGCGGGTCACATCATCCAACGCACCGAAGGGTTCATCCTGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTg                                    <  1:386497/149‑1 (MQ=255)
                                                                                                                acacGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACAGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTgg                                   >  5:358495/1‑149 (MQ=255)
                                                                                                                 cacGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTggg                                  <  6:320253/149‑1 (MQ=255)
                                                                                                                 cacGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTAATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTggg                                  >  6:108572/1‑149 (MQ=255)
                                                                                                                  acGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCACCGCGCTggcc                                 <  7:186969/149‑3 (MQ=255)
                                                                                                                          ggTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACAGAAGGGTTCATCCAGCAACAAGACTTCGCGCTGGCATCCCAGCCAGCCAGCCACCCCGCTTCGCAGTCCCATgccga                         >  7:342122/1‑148 (MQ=255)
                                                                                                                           gTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATgccgc                         >  5:373093/1‑148 (MQ=255)
                                                                                                                            tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                       <  3:224650/149‑1 (MQ=255)
                                                                                                                            tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                       <  2:43488/149‑1 (MQ=255)
                                                                                                                            tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                       <  6:272266/149‑1 (MQ=255)
                                                                                                                                gtgCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAg                      <  6:373093/146‑1 (MQ=255)
                                                                                                                                gtgCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGa                     <  3:273896/147‑1 (MQ=255)
                                                                                                                                       gCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTgcgc            >  8:67088/1‑149 (MQ=255)
                                                                                                                                        gcagcAGTTGCTGCTGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcg                                       <  3:133749/117‑1 (MQ=255)
                                                                                                                                        gcagcAGTTGCTGCTGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcg                                       >  4:133749/5‑121 (MQ=255)
                                                                                                                                              gTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTAc     >  5:79086/1‑149 (MQ=255)
                                                                                                                                              catGCAGCAGTTGCTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCgg                                                                         <  6:137741/79‑1 (MQ=255)
                                                                                                                                              catGCAGCAGTTGCTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCgg                                                                         >  5:137741/3‑81 (MQ=255)
                                                                                                                                               ttGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACt    >  5:392700/1‑149 (MQ=255)
                                                                                                                                                 gcagcagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTgg  >  5:217608/1‑149 (MQ=255)
                                                                                                                                                  cagcagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTgg  <  5:78616/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTGGTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGG  >  NC_002947/4133274‑4133567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑