Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,133,424 A→T 7.7% L182Q (CTG→CAG)  PP_3637 ← sulfonate ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,133,4240AT 7.7% 153.5 / 2.9 52L182Q (CTG→CAG) PP_3637sulfonate ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCAC  >  NC_002947/4133278‑4133571
                                                                                                                                                  |                                                                                                                                                   
gTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTgcagcag                                                                                                                                                    >  1:43488/1‑148 (MQ=255)
gTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTgcagcag                                                                                                                                                    >  6:331167/1‑148 (MQ=255)
   gctgCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCaa                                                                                                                                                 <  8:83942/145‑1 (MQ=255)
   gCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCaa                                                                                                                                                 <  1:336747/148‑1 (MQ=255)
      gCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGTCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAActgc                                                                                                                                             <  2:21115/149‑1 (MQ=255)
         ggTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACtgctgc                                                                                                                                          <  4:355266/149‑1 (MQ=255)
               tatGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCTGCTGCAActga                                                                                                                                             <  5:150882/140‑2 (MQ=255)
               tatGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCTGCTGCAActga                                                                                                                                             >  6:150882/1‑139 (MQ=255)
                  gCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGAGATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGgcg                                                                                                                                 >  8:186969/1‑149 (MQ=255)
                            gAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCAccg                                                                                                                       >  4:228813/1‑148 (MQ=255)
                            gAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCAc                                                                                                                         >  2:46159/1‑147 (MQ=255)
                            gAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCAc                                                                                                                         <  1:46159/147‑1 (MQ=255)
                                aTGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATc                                                                                                                   <  6:365454/149‑1 (MQ=255)
                                               ttGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAggg                                                                                                    <  3:269335/149‑1 (MQ=255)
                                                   ccAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCa                                                                                                >  3:115409/1‑149 (MQ=255)
                                                   ccAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCa                                                                                                >  6:78616/1‑149 (MQ=255)
                                                    cAGTAGCAGAACCCGGTCAGACAGCACCAGGGCTACGTCGATATCCTCAGTGATCAACACGGCCGCGGTCTTGTGCGAGGCGAACAGTTTCAGCAGCAACTGCTGCATCTCGGCGCGGCTCACCACATCCAACGCACCGCACGGTTCaa                                                                                               >  7:321233/1‑148 (MQ=255)
                                                     aGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATc                                                                                              <  4:203199/149‑1 (MQ=255)
                                                       tAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATc                                                                                              >  4:273896/1‑147 (MQ=255)
                                                            gAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGACTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGcaac                                                                                       <  5:331167/149‑1 (MQ=255)
                                                             aaCCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGcaac                                                                                       >  5:272266/1‑148 (MQ=255)
                                                              aCCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGcaacaa                                                                                     <  2:308820/149‑1 (MQ=255)
                                                                   gTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACtt                                                                                >  5:320253/1‑149 (MQ=255)
                                                                    tCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTc                                                                               <  2:234860/149‑1 (MQ=255)
                                                                     cTGACAGCAGCAGGGCTTCGTCGATATCGAGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCAAGTCGGCGCGGGTCACCACATCCAACGCAGCGAAGGGTTCATCCAGcaacaaccgttcg                                                                              >  2:386497/1‑142 (MQ=255)
                                                                        aCAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCgg                                                                             <  5:281083/147‑1 (MQ=255)
                                                                           gcagccgGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTgg                                                                        <  8:60535/149‑1 (MQ=255)
                                                                               cagGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTg                                                                    <  6:386033/149‑1 (MQ=255)
                                                                                 gggCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGcc                                                                  <  4:115409/149‑1 (MQ=255)
                                                                                       cgtcgATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGCCTTCGGGCTGGCGTGCCAGGCAg                                                            >  6:48934/1‑149 (MQ=255)
                                                                                         tcgATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCg                                                          <  7:90214/149‑1 (MQ=255)
                                                                                                       gaTCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgc                                            >  7:250869/1‑149 (MQ=255)
                                                                                                         tCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcgc                                          >  4:224650/1‑149 (MQ=255)
                                                                                                          cAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCaa                                                                                                                <  5:374863/78‑1 (MQ=255)
                                                                                                          cAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCaa                                                                                                                >  6:374863/1‑78 (MQ=255)
                                                                                                           aaCACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGGCGGCGCGGGTCACATCATCCAACGCACCGAAGGGTTCATCCTGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTg                                        <  1:386497/149‑1 (MQ=255)
                                                                                                            acacGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACAGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTgg                                       >  5:358495/1‑149 (MQ=255)
                                                                                                             cacGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTggg                                      <  6:320253/149‑1 (MQ=255)
                                                                                                             cacGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTAATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTggg                                      >  6:108572/1‑149 (MQ=255)
                                                                                                              acGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCACCGCGCTggcc                                     <  7:186969/149‑3 (MQ=255)
                                                                                                                      ggTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACAGAAGGGTTCATCCAGCAACAAGACTTCGCGCTGGCATCCCAGCCAGCCAGCCACCCCGCTTCGCAGTCCCATgccga                             >  7:342122/1‑148 (MQ=255)
                                                                                                                       gTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATgccgc                             >  5:373093/1‑148 (MQ=255)
                                                                                                                        tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                           <  6:272266/149‑1 (MQ=255)
                                                                                                                        tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                           <  2:43488/149‑1 (MQ=255)
                                                                                                                        tCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCa                           <  3:224650/149‑1 (MQ=255)
                                                                                                                            gtgCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAg                          <  6:373093/146‑1 (MQ=255)
                                                                                                                            gtgCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGa                         <  3:273896/147‑1 (MQ=255)
                                                                                                                                   gCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTgcgc                >  8:67088/1‑149 (MQ=255)
                                                                                                                                    gcagcAGTTGCTGCTGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcg                                           <  3:133749/117‑1 (MQ=255)
                                                                                                                                    gcagcAGTTGCTGCTGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTgcg                                           >  4:133749/5‑121 (MQ=255)
                                                                                                                                          gTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTAc         >  5:79086/1‑149 (MQ=255)
                                                                                                                                          catGCAGCAGTTGCTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCgg                                                                             <  6:137741/79‑1 (MQ=255)
                                                                                                                                          catGCAGCAGTTGCTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCgg                                                                             >  5:137741/3‑81 (MQ=255)
                                                                                                                                           ttGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACt        >  5:392700/1‑149 (MQ=255)
                                                                                                                                             gcagcagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTgg      >  5:217608/1‑149 (MQ=255)
                                                                                                                                              cagcagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTgg      <  5:78616/148‑1 (MQ=255)
                                                                                                                                                 cagcaACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATACAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCAGCCAGCCAACTGCGCCGGGTACTGGCCAc  >  6:372782/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTTGCGGCAGGTCGATATGCCACTGCCCGAGGATGTGCGCTGGGAGGTTGCCCAGTAGCAGAACCCGGTCTGACAGCAGCAGGGCTTCGTCGATATCGTGAGTGATCAACACGGCCGCGGTCTTGTGCGTGGCGATCAGTTGCAGCAGCAACTGCTGCATGTCGGCGCGGGTCACCTCATCCAACGCACCGAAGGGTTCATCCAGCAACAAGACTTCGGGCTGGCGTGCCAGGCAGCGGGCCAGCGCGGTGCGCTGGGCCATGCCGCCAGACAACTGCGCCGGGTACTGGCCAC  >  NC_002947/4133278‑4133571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑