Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,170,180 T→G 10.5% intergenic (‑288/‑99) katG ← / → PP_3669 catalase‑peroxidase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,170,1800TG10.5% 76.5 / 3.2 38intergenic (‑288/‑99)katG/PP_3669catalase‑peroxidase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (19/13);  new base G (2/2);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01

CACGCAGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGT  >  NC_002947/4170032‑4170327
                                                                                                                                                     |                                                                                                                                                   
cACGCAGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGt                                                                                                                                                     <  1:240989/149‑1 (MQ=255)
              cgcgCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGa                                                                                                                                       <  4:221595/149‑1 (MQ=255)
               gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCGCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                         >  1:80098/1‑147 (MQ=255)
               gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCAAGCCCGCGATGAg                                                                                                                                      >  1:165099/1‑149 (MQ=255)
                cgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                      <  1:140251/148‑1 (MQ=255)
                 gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGCCTTGACCGCGATGa                                                                                                                                       >  8:328730/1‑146 (MQ=255)
                 gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGCCTTGACCGCGATGa                                                                                                                                       <  7:328730/146‑1 (MQ=255)
                     cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCgg                                                                                                                                 >  4:299023/1‑148 (MQ=255)
                                  ttCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaag                                                                                                                   >  5:28138/1‑149 (MQ=255)
                                        gggCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaa                                                                                                             <  5:295310/149‑1 (MQ=255)
                                           ccACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                      <  6:393798/121‑1 (MQ=255)
                                           ccACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                      >  5:393798/1‑121 (MQ=255)
                                             aCGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCCGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGccc                                                                                                        >  7:340489/1‑149 (MQ=255)
                                                    aGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGCTGAGGGCGGCAGATCCATACAAGGCCCAAGGCCCCgcgcga                                                                                                 >  6:219878/1‑147 (MQ=255)
                                                     gACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCGGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCAt                                                                                                <  2:34259/149‑1 (MQ=255)
                                                        tAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCt                                                                                             >  4:74202/1‑149 (MQ=255)
                                                           c‑cGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTc                                                                                           >  2:38471/1‑148 (MQ=255)
                                                                 gttGGGCAGAGGTATAATAGTGGGGTTGTTGTCGCGTTTTTGGGTGGCTCTGTCCGGCGCAGGCCTGTATCGGCCTCATCGGGGTCAAGCCCGCGATGTGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATaca                                                                                     <  6:28138/148‑1 (MQ=255)
                                                                 cTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACAGCCATACAAGGCACAAGGCCCCGCGCCATGCGTCAATaca                                                                                     >  1:330586/1‑149 (MQ=255)
                                                                  ttGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATacac                                                                                    <  2:80098/149‑1 (MQ=255)
                                                                     ggCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGc                                                                                 >  6:302803/1‑149 (MQ=255)
                                                                     ggCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGc                                                                                 <  3:299023/149‑1 (MQ=255)
                                                                            gTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCCTAccc                                                                          >  6:152897/1‑149 (MQ=255)
                                                                             tCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTAAAGCCCGCGAAGAGGCCGGCACATCCATACAACGCACAAAGCCCCCCGCCATGCTTCAATAAACCTCCAAAccca                                                                         >  4:375240/1‑148 (MQ=255)
                                                                               tAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCttt                                                                       >  2:341360/1‑149 (MQ=255)
                                                                                 aTAGAGTGGCTGTTGGAGCCCGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGc                                                                     <  8:340489/149‑1 (MQ=255)
                                                                                             ttGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGa                                                         <  3:300884/149‑1 (MQ=255)
                                                                                               ggAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGc                                                       >  8:89845/1‑149 (MQ=255)
                                                                                                gAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGc                                                       <  7:89845/148‑1 (MQ=255)
                                                                                                   ccTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                         >  4:8629/1‑63 (MQ=255)
                                                                                                   ccTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                         <  3:8629/63‑1 (MQ=255)
                                                                                                           gctggctCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCAGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATAAACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCg                                           >  1:229214/1‑149 (MQ=255)
                                                                                                                     tCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGGGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTg                                 >  3:192874/1‑149 (MQ=255)
                                                                                                                     tCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCt                                  <  1:341360/148‑1 (MQ=255)
                                                                                                                                 cTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGAc                     <  1:38471/149‑1 (MQ=255)
                                                                                                                                       cGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACCCAGCCATACCCGTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGCCCTGCGCCGCGTCGACATGAAc               >  4:123256/1‑149 (MQ=255)
                                                                                                                                         gCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACct             >  6:211343/1‑149 (MQ=255)
                                                                                                                                               tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGt       >  8:193251/1‑149 (MQ=255)
                                                                                                                                                    gTCAAGCCCGCGATGGGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGt  <  4:192874/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CACGCAGGTGCTGACGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGT  >  NC_002947/4170032‑4170327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑