Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,170,183 A→T 12.7% intergenic (‑291/‑96) katG ← / → PP_3669 catalase‑peroxidase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,170,1830AT12.7% 83.2 / 4.4 38intergenic (‑291/‑96)katG/PP_3669catalase‑peroxidase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (20/13);  new base T (2/3);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGT  >  NC_002947/4170046‑4170327
                                                                                                                                          |                                                                                                                                                
cgcgCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGa                                                                                                                                       <  4:221595/149‑1 (MQ=255)
 gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCGCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                         >  1:80098/1‑147 (MQ=255)
 gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCAAGCCCGCGATGAg                                                                                                                                      >  1:165099/1‑149 (MQ=255)
  cgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                      <  1:140251/148‑1 (MQ=255)
   gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGCCTTGACCGCGATGa                                                                                                                                       >  8:328730/1‑146 (MQ=255)
   gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGCCTTGACCGCGATGa                                                                                                                                       <  7:328730/146‑1 (MQ=255)
       cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCgg                                                                                                                                 >  4:299023/1‑148 (MQ=255)
                    ttCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaag                                                                                                                   >  5:28138/1‑149 (MQ=255)
                          gggCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaa                                                                                                             <  5:295310/149‑1 (MQ=255)
                             ccACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                      >  5:393798/1‑121 (MQ=255)
                             ccACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                      <  6:393798/121‑1 (MQ=255)
                               aCGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCCGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGccc                                                                                                        >  7:340489/1‑149 (MQ=255)
                                      aGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGCTGAGGGCGGCAGATCCATACAAGGCCCAAGGCCCCgcgcga                                                                                                 >  6:219878/1‑147 (MQ=255)
                                       gACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCGGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCAt                                                                                                <  2:34259/149‑1 (MQ=255)
                                          tAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCt                                                                                             >  4:74202/1‑149 (MQ=255)
                                             c‑cGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTc                                                                                           >  2:38471/1‑148 (MQ=255)
                                                   gttGGGCAGAGGTATAATAGTGGGGTTGTTGTCGCGTTTTTGGGTGGCTCTGTCCGGCGCAGGCCTGTATCGGCCTCATCGGGGTCAAGCCCGCGATGTGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATaca                                                                                     <  6:28138/148‑1 (MQ=255)
                                                   cTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACAGCCATACAAGGCACAAGGCCCCGCGCCATGCGTCAATaca                                                                                     >  1:330586/1‑149 (MQ=255)
                                                    ttGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATacac                                                                                    <  2:80098/149‑1 (MQ=255)
                                                       ggCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGc                                                                                 >  6:302803/1‑149 (MQ=255)
                                                       ggCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGc                                                                                 <  3:299023/149‑1 (MQ=255)
                                                              gTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCCTAccc                                                                          >  6:152897/1‑149 (MQ=255)
                                                               tCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTAAAGCCCGCGAAGAGGCCGGCACATCCATACAACGCACAAAGCCCCCCGCCATGCTTCAATAAACCTCCAAAccca                                                                         >  4:375240/1‑148 (MQ=255)
                                                                 tAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCttt                                                                       >  2:341360/1‑149 (MQ=255)
                                                                   aTAGAGTGGCTGTTGGAGCCCGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGc                                                                     <  8:340489/149‑1 (MQ=255)
                                                                               ttGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGa                                                         <  3:300884/149‑1 (MQ=255)
                                                                                 ggAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGc                                                       >  8:89845/1‑149 (MQ=255)
                                                                                  gAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGc                                                       <  7:89845/148‑1 (MQ=255)
                                                                                     ccTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                         >  4:8629/1‑63 (MQ=255)
                                                                                     ccTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                         <  3:8629/63‑1 (MQ=255)
                                                                                             gctggctCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCAGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATAAACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCg                                           >  1:229214/1‑149 (MQ=255)
                                                                                                       tCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGGGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTg                                 >  3:192874/1‑149 (MQ=255)
                                                                                                       tCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCt                                  <  1:341360/148‑1 (MQ=255)
                                                                                                                   cTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGAc                     <  1:38471/149‑1 (MQ=255)
                                                                                                                         cGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACCCAGCCATACCCGTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGCCCTGCGCCGCGTCGACATGAAc               >  4:123256/1‑149 (MQ=255)
                                                                                                                           gCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACct             >  6:211343/1‑149 (MQ=255)
                                                                                                                                 tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGt       >  8:193251/1‑149 (MQ=255)
                                                                                                                                      gTCAAGCCCGCGATGGGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGt  <  4:192874/149‑1 (MQ=255)
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CGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGT  >  NC_002947/4170046‑4170327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑