Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,170,185 C→A 12.6% intergenic (‑293/‑94) katG ← / → PP_3669 catalase‑peroxidase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,170,1850CA12.6% 98.7 / 4.4 39intergenic (‑293/‑94)katG/PP_3669catalase‑peroxidase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (20/14);  new base A (2/3);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTCGAAG  >  NC_002947/4170046‑4170333
                                                                                                                                            |                                                                                                                                                    
cgcgCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGa                                                                                                                                             <  4:221595/149‑1 (MQ=255)
 gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGCGCGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                               >  1:80098/1‑147 (MQ=255)
 gcgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCAAGCCCGCGATGAg                                                                                                                                            >  1:165099/1‑149 (MQ=255)
  cgcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                            <  1:140251/148‑1 (MQ=255)
   gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGCCTTGACCGCGATGa                                                                                                                                             >  8:328730/1‑146 (MQ=255)
   gcTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGCCTTGACCGCGATGa                                                                                                                                             <  7:328730/146‑1 (MQ=255)
       cccTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCgg                                                                                                                                       >  4:299023/1‑148 (MQ=255)
                    ttCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaag                                                                                                                         >  5:28138/1‑149 (MQ=255)
                          gggCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATacaaggcacaa                                                                                                                   <  5:295310/149‑1 (MQ=255)
                             ccACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                            >  5:393798/1‑121 (MQ=255)
                             ccACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGGCTTGACCGCGATGAg                                                                                                                                            <  6:393798/121‑1 (MQ=255)
                               aCGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCCGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGccc                                                                                                              >  7:340489/1‑149 (MQ=255)
                                      aGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGCTGAGGGCGGCAGATCCATACAAGGCCCAAGGCCCCgcgcga                                                                                                       >  6:219878/1‑147 (MQ=255)
                                       gACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCGGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCAt                                                                                                      <  2:34259/149‑1 (MQ=255)
                                          tAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCt                                                                                                   >  4:74202/1‑149 (MQ=255)
                                             c‑cGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTc                                                                                                 >  2:38471/1‑148 (MQ=255)
                                                   gttGGGCAGAGGTATAATAGTGGGGTTGTTGTCGCGTTTTTGGGTGGCTCTGTCCGGCGCAGGCCTGTATCGGCCTCATCGGGGTCAAGCCCGCGATGTGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATaca                                                                                           <  6:28138/148‑1 (MQ=255)
                                                   cTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACAGCCATACAAGGCACAAGGCCCCGCGCCATGCGTCAATaca                                                                                           >  1:330586/1‑149 (MQ=255)
                                                    ttGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATacac                                                                                          <  2:80098/149‑1 (MQ=255)
                                                       ggCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGc                                                                                       >  6:302803/1‑149 (MQ=255)
                                                       ggCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGc                                                                                       <  3:299023/149‑1 (MQ=255)
                                                              gTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCCTAccc                                                                                >  6:152897/1‑149 (MQ=255)
                                                               tCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTAAAGCCCGCGAAGAGGCCGGCACATCCATACAACGCACAAAGCCCCCCGCCATGCTTCAATAAACCTCCAAAccca                                                                               >  4:375240/1‑148 (MQ=255)
                                                                 tAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCttt                                                                             >  2:341360/1‑149 (MQ=255)
                                                                   aTAGAGTGGCTGTTGGAGCCCGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGc                                                                           <  8:340489/149‑1 (MQ=255)
                                                                               ttGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGa                                                               <  3:300884/149‑1 (MQ=255)
                                                                                 ggAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGc                                                             >  8:89845/1‑149 (MQ=255)
                                                                                  gAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGc                                                             <  7:89845/148‑1 (MQ=255)
                                                                                     ccTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                               >  4:8629/1‑63 (MQ=255)
                                                                                     ccTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGAt                                                                                                                                               <  3:8629/63‑1 (MQ=255)
                                                                                             gctggctCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCAGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATAAACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCg                                                 >  1:229214/1‑149 (MQ=255)
                                                                                                       tCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGGGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTg                                       >  3:192874/1‑149 (MQ=255)
                                                                                                       tCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCt                                        <  1:341360/148‑1 (MQ=255)
                                                                                                                   cTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGAc                           <  1:38471/149‑1 (MQ=255)
                                                                                                                         cGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACCCAGCCATACCCGTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGCCCTGCGCCGCGTCGACATGAAc                     >  4:123256/1‑149 (MQ=255)
                                                                                                                           gCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACct                   >  6:211343/1‑149 (MQ=255)
                                                                                                                                 tCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGt             >  8:193251/1‑149 (MQ=255)
                                                                                                                                      gTCAAGCCCGCGATGGGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGt        <  4:192874/149‑1 (MQ=255)
                                                                                                                                           gCCCGCGCTGAGGCCTGCACCTCCCTACCAGGCCCAAGGCCCCGCGCCATGCTTCAATCCCCAGCCCTCCCCTTGGCCCCGGACGCCGAGCCCTCATGAACCGCAACGACCGGCGCCGCGTCGACATGACCCTGCTGGTGCTGTTCGaa   <  6:311950/149‑1 (MQ=255)
                                                                                                                                            cccGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGCCCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTCGAAg  >  2:323719/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
CGCGCTGCCCTGTCGCGGAATTCGCGGGGCCACGAGAAAGACTAGC‑CGAGCTTGGGCAGAGGTCTAATAGAGTGGCTGTTGGAGCCTGATAGGCTGGCTCTGTCAGGCGCAGGCCTGTATCGGCCTCATCGCGGTCAAGCCCGCGATGAGGCCGGCACATCCATACAAGGCACAAGGCCCCGCGCCATGCTTCAATACACAGCCATACCCTTTGCCCAGGACGCCGAGCCCTCATGAACCGCAACGACCTGCGCCGCGTCGACATGAACCTGCTGGTGCTGTTCGAAG  >  NC_002947/4170046‑4170333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑