Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,242,999 T→C 7.5% intergenic (‑163/+31) PP_3719 ← / ← PP_3720 putrescine‑binding protein/NAD(P)H quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,242,9990TC 7.5% 146.0 / 2.1 53intergenic (‑163/+31)PP_3719/PP_3720putrescine‑binding protein/NAD(P)H quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (24/25);  new base C (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

GAGTGCTCCGGATCGAGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATTGCTGCAGAAAGTCCTGC  >  NC_002947/4242853‑4243141
                                                                                                                                                  |                                                                                                                                              
gAGTGCTCCGGATCGAGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGc                                                                                                                                              <  6:98020/149‑1 (MQ=255)
 aGTGCTCCGGATCGAGCCGATGGGTTCACTATCGGTGGATTCGCCAAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCACGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCg                                                                                                                                             >  3:310703/1‑149 (MQ=255)
        cGGATCGAGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAg                                                                                                                                      >  4:325837/1‑149 (MQ=255)
        cGGATCGAGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGGGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCAACGCGGCCCCaa                                                                                                                                      <  8:147529/149‑2 (MQ=255)
           aTCGAGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGG‑CCCAGTGGt                                                                                                                                  <  8:12546/149‑1 (MQ=255)
               aGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCaa                                                                                                                               <  4:310703/149‑1 (MQ=255)
                 ccGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATa                                                                                                                             <  6:78856/149‑1 (MQ=255)
                   gATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATa                                                                                                                             >  4:276799/1‑147 (MQ=255)
                   gATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACa                                                                                                                           <  5:340824/149‑1 (MQ=255)
                   gATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACa                                                                                                                           <  3:276799/149‑1 (MQ=255)
                    aTGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAg                                                                                                                          <  7:292166/149‑1 (MQ=255)
                        gTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGgag                                                                                                                       >  2:146177/1‑148 (MQ=255)
                         ttCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGgaga                                                                                                                      >  4:262071/1‑148 (MQ=255)
                                      gATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGc                                                                                                        >  1:250605/1‑149 (MQ=255)
                                      gATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGc                                                                                                        >  5:349479/1‑149 (MQ=255)
                                       aTTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCg                                                                                                       <  1:148636/149‑1 (MQ=255)
                                        ttCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCgg                                                                                                      >  3:199722/1‑149 (MQ=255)
                                           gCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTa                                                                                                   >  5:123590/1‑149 (MQ=255)
                                           gCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTa                                                                                                   >  5:4537/1‑149 (MQ=255)
                                           gCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTa                                                                                                   <  4:199722/149‑1 (MQ=255)
                                              cAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACaa                                                                                                <  1:228249/149‑1 (MQ=255)
                                               aGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACaa                                                                                                <  2:350435/148‑1 (MQ=255)
                                               aGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACaa                                                                                                >  1:350435/1‑148 (MQ=255)
                                                    tGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCtt                                                                                           >  5:324360/1‑148 (MQ=255)
                                                      aCATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCa                                                                                         <  3:325837/148‑1 (MQ=255)
                                                        aTACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCg                                                                                      <  6:324360/149‑1 (MQ=255)
                                                          aCCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGa                                                                                     >  7:316877/1‑148 (MQ=255)
                                                          aCCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCAACGCGGcc                                                                                                                                          <  2:30732/95‑1 (MQ=255)
                                                          aCCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCAACGCGGcc                                                                                                                                          >  1:30732/1‑95 (MQ=255)
                                                            cccGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGCCAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCCCGTCAGCGGGTACAACGCGTCATctcact                                                                                  >  4:238707/1‑144 (MQ=255)
                                                            cccGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACt                                                                                  >  6:20153/1‑149 (MQ=255)
                                                             ccGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTg                                                                                 >  3:95938/1‑149 (MQ=255)
                                                             ccGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCAGACAAAATGTTGGGGCCGCATTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTg                                                                                 >  7:249579/1‑149 (MQ=255)
                                                                     gtgggtggTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGgc                                                                         >  1:295855/1‑149 (MQ=255)
                                                                          tggtCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCggg                                                                    <  3:262071/149‑1 (MQ=255)
                                                                             tCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAac                                                                 <  8:316877/149‑1 (MQ=255)
                                                                               gTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGCacac                                                               >  4:46135/1‑149 (MQ=255)
                                                                                           gTGGCGCTGGAAGGTTGGCAGAACATCGTATGCCGTCAAAAGGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGACAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGgtc                                                   <  8:284670/149‑1 (MQ=255)
                                                                                               cgcTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGcgcg                                                                      >  6:374164/1‑126 (MQ=255)
                                                                                               cgcTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGcgcg                                                                      <  5:374164/126‑1 (MQ=255)
                                                                                               cgcTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTcc                                               <  4:95938/149‑1 (MQ=255)
                                                                                                        gTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAgctgc                                      <  6:4537/149‑1 (MQ=255)
                                                                                                            gCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTcc                                   <  6:349479/148‑1 (MQ=255)
                                                                                                                 aCATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGc                             <  5:20153/149‑1 (MQ=255)
                                                                                                                   aTCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAacac                                                               >  2:238012/1‑113 (MQ=255)
                                                                                                                    tCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGc                          >  4:230998/1‑149 (MQ=255)
                                                                                                                      gTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCg                        <  6:123590/149‑1 (MQ=255)
                                                                                                                               cAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATtgctg               <  8:249579/149‑1 (MQ=255)
                                                                                                                                aaaaTGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATtgctgc              <  1:146177/149‑1 (MQ=255)
                                                                                                                                      ttGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATTGCTGCAGaaa         <  3:230998/148‑1 (MQ=255)
                                                                                                                                      ttGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCAGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATTGCTGCAGAAAg        >  1:102161/1‑149 (MQ=255)
                                                                                                                                       tGGGGCCGCAACGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATTGCTGCAGAAAGt       >  4:247851/1‑149 (MQ=255)
                                                                                                                                            ccGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATTGCTGCAGAAAGTCCTGc  >  3:230205/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GAGTGCTCCGGATCGAGCCGATGGGTTCACTATCGGTGGATTCGCCCAGGGGTGACATACCCCGAATGTGTGGGTGGTCGTTTTTTGGCAGGTGGCGCTGGAAGGTTTGCAGAACATCGTATGCCGACAAAATGTTGGGGCCGCGTTGCGGCCCCAGTGGTCAATACAGGAGAAAGGTCACGTCAGCGGGTACAACGCTTCATCGAACTGCGCCAGGCGCGGGAACACCAGTGGCTGGTCGTCCGAGAGCTGCTCCAGACGCTGCCGGTATTGCTGCAGAAAGTCCTGC  >  NC_002947/4242853‑4243141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑