Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,246,188	C→G	9.8%	S29S (TCG→TCC)	alr ←	alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,246,188	0	C	G	9.8%	170.7 / 9.2	61	S29S (TCG→TCC)	alr	alanine racemase
Reads supporting (aligned to +/- strand):  ref base C (27/28);  new base G (4/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

AGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTT  >  NC_002947/4246040‑4246334                                                                                                                                                     |                                                                                                                                                   aGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATc                                                                                                                                                    >  4:190366/1‑149 (MQ=255)     aTCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGaa                                                                                                                                                 >  3:320421/1‑147 (MQ=255)     aTCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGAc                                                                                                                                                 >  7:197019/1‑148 (MQ=255)          ccTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATGGACAACggc                                                                                                                                           <  5:163191/149‑2 (MQ=255)              gAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATGGACAACggcac                                                                                                                                         <  4:168358/147‑4 (MQ=255)              gAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGAc                                                                                                                                                 >  6:386988/1‑139 (MQ=255)              gAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGAc                                                                                                                                                 <  5:386988/139‑1 (MQ=255)              gAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACggtgg                                                                                                                                         <  5:328988/147‑1 (MQ=255)              gAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGgtggtg                                                                                                                                       >  1:346490/1‑149 (MQ=255)                gCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGcc                                                                                                                                     <  6:271855/149‑1 (MQ=255)                     gcgcACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATa                                                                                                                                <  1:372287/149‑1 (MQ=255)                          caGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAggg                                                                                                                           <  1:156376/149‑1 (MQ=255)                           aGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAggg                                                                                                                           <  4:57534/148‑1 (MQ=255)                           aGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAggg                                                                                                                           >  3:57534/1‑148 (MQ=255)                                ggACTTGCCGGCCAGCTCGGCCTGCAGCGTGCTGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                       <  7:78780/147‑1 (MQ=255)                                  aCTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                       <  5:348555/145‑1 (MQ=255)                                  aCTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                       >  6:348555/1‑145 (MQ=255)                                   cTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTc                                                                                                                  >  8:93821/1‑149 (MQ=255)                                       ccGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGt                                                                                                              <  7:293945/149‑1 (MQ=255)                                        cGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTg                                                                                                             <  3:190366/149‑1 (MQ=255)                                        cGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTg                                                                                                             <  1:33583/149‑1 (MQ=255)                                          gCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGa                                                                                                            >  6:349799/1‑148 (MQ=255)                                           ccAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATc                                                                                                          <  5:349799/149‑1 (MQ=255)                                            cAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCa                                                                                                         <  4:221920/149‑1 (MQ=255)                                               ctgggCCTGCAGCGTGCGGATGTGGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTTCCGTTTTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                      <  6:352034/146‑1 (MQ=255)                                               cTCGGCCTGCAGCGTGCGGATGTGGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                      <  4:134017/149‑1 (MQ=255)                                                tCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCAGCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAg                                                                                                     >  8:107261/1‑149 (MQ=255)                                                  ggCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCACCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAg                                                                                                     <  1:285672/147‑1 (MQ=255)                                                            gTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGc                                                                                         <  3:116106/149‑1 (MQ=255)                                                              gCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACA‑GGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGc                                                                                      <  7:6209/149‑1 (MQ=255)                                                                   tgttgtGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAg                                                                                   >  5:221813/1‑148 (MQ=255)                                                                      tgtgCTGCAGAGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAg                                                                                <  7:134700/148‑1 (MQ=255)                                                                       gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAggg                                                                              >  3:232257/1‑149 (MQ=255)                                                                        tgctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAggg                                                                              <  7:94951/148‑1 (MQ=255)                                                                          ctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTa                                                                            <  1:87055/148‑1 (MQ=255)                                                                                   gctggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAgg                                                                  <  3:240729/149‑1 (MQ=255)                                                                                   gctggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAgg                                                                  >  1:89379/1‑149 (MQ=255)                                                                                   gctggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAgg                                                                  >  7:28890/1‑149 (MQ=255)                                                                                         gctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATgg                                                            >  8:313393/1‑149 (MQ=255)                                                                                                    cccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgt                                                 >  4:306017/1‑149 (MQ=255)                                                                                                    cccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgt                                                 >  1:380985/1‑149 (MQ=255)                                                                                                    cccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgt                                                 >  7:113134/1‑149 (MQ=255)                                                                                                     ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtg                                                >  6:304054/1‑149 (MQ=255)                                                                                                     ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtg                                                >  2:44150/1‑149 (MQ=255)                                                                                                           aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTg                                          >  8:42267/1‑149 (MQ=255)                                                                                                              gCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTc                                        <  6:221813/148‑1 (MQ=255)                                                                                                                              aGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTaa                        <  7:93821/148‑1 (MQ=255)                                                                                                                               gggTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACa                      <  1:218866/149‑1 (MQ=255)                                                                                                                                      ggTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg               >  4:318165/1‑149 (MQ=255)                                                                                                                                       gTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAgg              >  2:162268/1‑149 (MQ=255)                                                                                                                                        caccGTTGTCGATGGACAACGGTGGTGCCGCCTACAGGGGGGCCGGTCCGGTGATCAGCAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGATAGGGCATGGTGTTTACTCGTGTTCTTGTCGAAGTGCGCGCCTTAACAGACGCAgct             >  6:110593/3‑147 (MQ=255)                                                                                                                                        caccGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgt             >  8:259916/3‑149 (MQ=255)                                                                                                                                         accGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg            >  3:284437/2‑149 (MQ=255)                                                                                                                                         accGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCAAGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg            >  6:336422/2‑149 (MQ=255)                                                                                                                                            gTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtgt         >  6:68557/1‑149 (MQ=255)                                                                                                                                            gTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtgt         <  1:44150/149‑1 (MQ=255)                                                                                                                                                tCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGa     <  2:380985/149‑1 (MQ=255)                                                                                                                                                  cATCGACAACGGTGGTGCCGCATACAGGGGGGCCAGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTAGTAGTGTTCTTGTCGTAGTGCGAGCCTTAACAGACGCAGGTGTGttt       >  6:314018/1‑145 (MQ=255)                                                                                                                                                   aTCGACAACGTTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGa     <  3:369772/146‑1 (MQ=255)                                                                                                                                                   aTCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGa     >  4:369772/1‑146 (MQ=255)                                                                                                                                                   aTCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  7:832/1‑149 (MQ=255)                                                                                                                                                   aTCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGAGCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  3:95628/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTT  >  NC_002947/4246040‑4246334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑