Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,385,288 A→C 6.8% V83G (GTT→GGT)  PP_3860 ← FluMu protein gp47

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,385,2880AC 6.8% 173.8 / 2.8 58V83G (GTT→GGT) PP_3860FluMu protein gp47
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base C (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

CGCGCACCGGCACCTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTAAGAACCCGCGAAG  >  NC_002947/4385142‑4385432
                                                                                                                                                   |                                                                                                                                                  
cgcgcACCGGCACCTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑Ca                                                                                                                                                 <  4:41312/149‑1 (MQ=255)
   gcACCGGCACCTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAg‑cc                                                                                                                                             >  1:127505/1‑149 (MQ=255)
       ccgCACCTGCGCCGTACCATCCACCACACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGTTC‑CAg‑ccgcag                                                                                                                                         <  1:332772/147‑3 (MQ=255)
        ggCACCTGCGACGTACGATCCAGCACACGCGTCGTTACGGTCACGTCGT‑ATCGGCGGCCGTCCTTATGGTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACATTGGGGCGTGCGGCGGTTC‑CAg‑ccgcagg                                                                                                                                        <  8:297280/149‑4 (MQ=255)
         gCACCTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGTGAGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCtt                                                                                                                                        >  1:90622/1‑149 (MQ=255)
           aCCTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTgcg                                                                                                                                     >  6:171916/1‑149 (MQ=255)
            ccTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTgcgc                                                                                                                                    <  5:298984/149‑1 (MQ=255)
                 gcCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTc                                                                                                                               >  2:263151/1‑149 (MQ=255)
                    gTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCtt                                                                                                                            <  2:285266/149‑1 (MQ=255)
                     tACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCtt                                                                                                                            >  3:141350/1‑148 (MQ=255)
                       ccttCCACAAGACGCTTCGTTACGGTCAAGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCTTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTaa                                                                                                                          <  3:243681/145‑1 (MQ=255)
                       ccATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAGGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAg                                                                                                                         <  2:142712/149‑1 (MQ=255)
                       ccATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCTCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTaa                                                                                                                          <  1:343453/148‑1 (MQ=255)
                       ccATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTaa                                                                                                                          <  5:235862/148‑1 (MQ=255)
                       ccATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAg                                                                                                                         <  5:309111/149‑1 (MQ=255)
                       ccATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAg                                                                                                                         >  3:149669/1‑149 (MQ=255)
                         aTCCACCCGACGCGGCGGTACGGGCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGgc                                                                                                                       <  4:293947/149‑1 (MQ=255)
                            caccaGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGgcgc                                                                                                                     <  4:141350/148‑1 (MQ=255)
                            caccaGACGCGTCGTTACGGTCACAACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGCCCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGCGCCTGCGGCTGGAA‑CAC‑CCGCCTTGCGCCCGTCCTTATGgccag                                                                                                                    >  5:53619/1‑146 (MQ=255)
                             accaGACGCGTCTTTACTGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGTCGTTATTAGCGGCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCa                                                                                                                   <  6:204881/149‑1 (MQ=255)
                             accaGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCa                                                                                                                   >  8:231039/1‑149 (MQ=255)
                               caGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCAtt                                                                                                                 <  5:171916/149‑1 (MQ=255)
                                    gcgTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGcc                                                                                                            >  7:272794/1‑149 (MQ=255)
                                      gtcgtTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGCCCCa                                                                                                          <  1:263151/149‑1 (MQ=255)
                                               gTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAACCAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTcagca                                                                                                  <  1:286130/149‑1 (MQ=255)
                                                  acgacgT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCAt                                                                                              <  6:331257/149‑1 (MQ=255)
                                                     acgT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCAtctc                                                                                           <  3:144021/149‑1 (MQ=255)
                                                       gT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCtt                                                                                         >  1:382227/1‑149 (MQ=255)
                                                       gT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCtt                                                                                         <  4:149669/149‑1 (MQ=255)
                                                           gcggcgGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCAt                                                                                      >  7:228866/1‑149 (MQ=255)
                                                                      ccTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGcc                                                                           >  2:81696/1‑149 (MQ=255)
                                                                      ccTGATGCTGGTAGACCATACCGGCATCAACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCg                                                                             >  5:55526/1‑147 (MQ=255)
                                                                           tGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTg                                                                      >  6:198561/1‑149 (MQ=255)
                                                                           tGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTg                                                                      <  7:231039/149‑1 (MQ=255)
                                                                                gTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGa                                                                   <  2:90622/147‑1 (MQ=255)
                                                                                   gACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCa                                                                                       >  6:32999/1‑124 (MQ=255)
                                                                                   gACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCa                                                                                       <  5:32999/124‑1 (MQ=255)
                                                                                     ccATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCcgc                                                            >  2:41497/1‑149 (MQ=255)
                                                                                     ccATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCcgc                                                            <  2:127505/149‑1 (MQ=255)
                                                                                           cGGCATCCACCAAGGCGTTATTACCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGTGACTGCCGCGCGAt                                                       >  8:348043/1‑148 (MQ=255)
                                                                                            ggCATCCACCAAGGCGTTATTAGCGCCGCTGCCGACAATGGGGCCTGCGGCTGGAA‑CCG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCCTCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCCCGATcc                                                     >  3:81171/1‑149 (MQ=255)
                                                                                                 ccaccaAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTcc                                                >  5:295843/1‑149 (MQ=255)
                                                                                                  caccaAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCt                                               >  3:281283/1‑149 (MQ=255)
                                                                                                    ccaAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGa                                             >  3:322357/1‑149 (MQ=255)
                                                                                                          cGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCGGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCa                                       <  8:272794/149‑1 (MQ=255)
                                                                                                           gTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCa                                       >  3:67151/1‑148 (MQ=255)
                                                                                                                   cgccgcTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCg                              >  1:193851/1‑149 (MQ=255)
                                                                                                                     ccgcTGACGACAATGGGGCCTGCGGCTGGAA‑CAGACCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCAtctc                                                                                           >  1:53142/1‑87 (MQ=255)
                                                                                                                     ccgcTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCAtctc                                                                                           <  2:53142/86‑1 (MQ=255)
                                                                                                                           acgacAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTcc                                                <  8:276083/123‑1 (MQ=255)
                                                                                                                             gacAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGgcgc                    <  1:41497/149‑1 (MQ=255)
                                                                                                                                aaTGGGGCCTGCGGCGGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCATCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCAc                 <  6:55526/149‑1 (MQ=255)
                                                                                                                                 aTGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCATCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACt                <  7:348043/149‑1 (MQ=255)
                                                                                                                                    gggCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTaa              <  2:382227/148‑1 (MQ=255)
                                                                                                                                    gggCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTaa              <  1:81696/148‑1 (MQ=255)
                                                                                                                                      caaggCGGCTGTTC‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTAAGaa           >  5:257281/5‑149 (MQ=255)
                                                                                                                                       aaggCGGCTGTTC‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTAAGAAc          >  1:349002/4‑149 (MQ=255)
                                                                                                                                                 gAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTAAGAACCCGCGAAg  <  7:157957/147‑1 (MQ=255)
                                                                                                                                                   a‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATa                                     >  6:126537/1‑110 (MQ=255)
                                                                                                                                                   a‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATa                                     <  5:126537/110‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCGCACCGGCACCTGCGCCGTACCATCCACCAGACGCGTCGTTACGGTCACGACGT‑AGCGGCGGCCGTCCTGATGCTGGTAGACCATACCGGCATCCACCAAGGCGTTATTAGCGCCGCTGACGACAATGGGGCCTGCGGCTGGAA‑CAG‑CCGCCTTGCGCCCGTCCTTAAGGCGCCATTCGGCCCATTTCAGCAGCATCTCTTCATCGCAGGTCGCCGGGTGGGACTGCCGCGCGATCCACTCCTGATGGCCATACAGACCGTAGGCGGCGCCACTAAGAACCCGCGAAG  >  NC_002947/4385142‑4385432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑