Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,446,491 G→C 13.4% Y358* (TAC→TAG)  nicC ← 6‑hydroxynicotinate 3‑monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,446,4910GC13.4% 99.5 / 8.6 39Y358* (TAC→TAG) nicC6‑hydroxynicotinate 3‑monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (17/16);  new base C (2/3);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

CCTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATG  >  NC_002947/4446344‑4446635
                                                                                                                                                   |                                                                                                                                                  
ccTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTg                                                                                                                                                    >  6:43061/1‑148 (MQ=255)
         cATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTCTACAGCCAgg                                                                                                                                          <  8:182100/149‑1 (MQ=255)
          aTAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTCTACAGCCAgga                                                                                                                                         <  6:392683/149‑2 (MQ=255)
           tAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTTTACAGCCAggaa                                                                                                                                        <  8:98283/149‑3 (MQ=255)
                    tAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTg                                                                                                                              <  5:184947/149‑1 (MQ=255)
                      aTGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGa                                                                                                                            <  3:103618/149‑1 (MQ=255)
                         cTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTCGCTGTAGAGCCAGGTGTTGGCG‑TTGGATAc                                                                                                                         <  1:33163/149‑1 (MQ=255)
                             cGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCATCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGAt                                                                                                                     >  1:292077/1‑149 (MQ=255)
                                    cATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTATAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCt                                                                                                              <  2:335159/149‑1 (MQ=255)
                                    cATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCt                                                                                                              >  2:83617/1‑149 (MQ=255)
                                    cATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGATGCTGCCCATAGAGGTCGTAGACGTAAACACAGGCTGGATCTTCCTGGCTGTAGAGCAAGGTGTTGGCG‑TTGGATACAGATTGCACCt                                                                                                              >  5:213638/1‑149 (MQ=255)
                                                       accaGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCg                                                                                          <  1:184148/149‑1 (MQ=255)
                                                       accaGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCg                                                                                          <  2:90072/149‑1 (MQ=255)
                                                          aGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGAACCTG‑CGATGCCCGCTCTTTGCGGtt                                                                                       >  2:196677/1‑149 (MQ=255)
                                                           gCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTg                                                                                      <  8:233701/149‑1 (MQ=255)
                                                                 aaGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCa                                                                                <  7:65867/149‑1 (MQ=255)
                                                                 aaGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACAGATTGCACCTGCCGATGCCCGCTCTTTGCGGTTGGCTTc                                                                                 <  1:202112/149‑1 (MQ=255)
                                                                   ggTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATa                                                                              <  1:83617/149‑1 (MQ=255)
                                                                    gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑tt                                                                                                                               <  6:300552/100‑1 (MQ=255)
                                                                    gTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑tt                                                                                                                               >  5:300552/1‑100 (MQ=255)
                                                                       cTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAAc                                                                          >  3:15581/1‑149 (MQ=255)
                                                                                    cccGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGc                                                             <  4:15581/149‑1 (MQ=255)
                                                                                     ccGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCg                                                            >  1:355279/1‑149 (MQ=255)
                                                                                                  gctgcCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGc                                               >  6:228720/1‑149 (MQ=255)
                                                                                                      cccATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCgg                                           >  7:86248/1‑149 (MQ=255)
                                                                                                            gagGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCGTTTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTcc                                      >  1:305528/1‑149 (MQ=255)
                                                                                                               gTCGTAGCCGTAAACACAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGTCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGACGGACAGACCGGTCTACTg                                    >  8:303879/1‑147 (MQ=255)
                                                                                                                  gTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCa                                                                                <  7:357716/100‑1 (MQ=255)
                                                                                                                  gTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCa                                                                                >  8:357716/1‑100 (MQ=255)
                                                                                                                   gaGCCGTATACGCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGGG‑TTGGATACCGATTGCACCTG‑TGATGCGCGCTCTATGCGGTTGGCTTCATAGAACGCGATCGCCGTGCGGTGGTCTGACAGCCCGGTCTCCTgcaggca                              <  6:213638/148‑1 (MQ=255)
                                                                                                                           aaCCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTTGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCa                      <  2:355279/149‑1 (MQ=255)
                                                                                                                               cAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCAt                  >  2:103852/1‑149 (MQ=255)
                                                                                                                                aGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGAGACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATg                 <  8:86248/149‑1 (MQ=255)
                                                                                                                                  gCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGc               >  5:235970/1‑149 (MQ=255)
                                                                                                                                       caacaccTGGCTCTACAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGt          >  3:218203/6‑149 (MQ=255)
                                                                                                                                       aTCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGt          >  3:368097/1‑149 (MQ=255)
                                                                                                                                        tCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTc         >  3:95305/1‑149 (MQ=255)
                                                                                                                                            ccTGGCTCTACAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCg     <  4:218203/149‑1 (MQ=255)
                                                                                                                                             cTGGCTCTACAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGa    >  7:27847/1‑149 (MQ=255)
                                                                                                                                               ggCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATg  >  3:8619/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTTGCCGCCATAGCGCAGGTAATGCTGGCGGATGCCATTGGCGCTGACGTTACCACCAGCGACGAAGGTGCTCATGCCGCCTCCCCGCTTTCAAGCTGCTGCCCATAGAGGTCGTAGCCGTAAACCCAGGCTGGATCTTCCTGGCTGTAGAGCCAGGTGTTGGCG‑TTGGATACCGATTGCACCTG‑CGATGCCCGCTCTTTGCGGTTGGCTTCATACAACGCGAACGCCGTGCGGTGGTCGGACAGCCCGGTCTCCTGCAGGCAGCGGGTCAGCATGGCCGCGTCCTCGATG  >  NC_002947/4446344‑4446635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑