| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 100.0% | 120.6 / NA | 42 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/25); total (17/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 121.4 / NA | 42 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (17/25); total (17/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAA > NC_002947/1499342‑1499610 || cGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc < 4:9781/149‑1 (MQ=255)cGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc < 2:102668/149‑1 (MQ=255) ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgt < 2:288230/149‑1 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGGGCATGGCACCCGCGTTGCCGgtg > 1:294472/1‑149 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCGTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGgtg > 1:276061/1‑149 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGt < 7:288281‑M2/149‑2 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc < 5:60024/136‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc > 6:60024/1‑136 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg > 1:298277‑M2/1‑141 (MQ=255) agagCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaca > 7:348977‑M2/1‑110 (MQ=255) agagCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCGTTGCCCGTGttagcgggcacgcccgcgcccaca < 8:348977‑M2/134‑25 (MQ=255) c‑TGAACGACGCCTTGGCACCACAGCAGGCCTGAGGCTGCCAAACGGGGGGCGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑gg < 1:268729/149‑1 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTAt > 5:354794/1‑149 (MQ=21) gCCTTGGCCCCACAGCAGGACTGTGGCGGCCAAACGGTGGGAGCGGGCGTGCCGGCTAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGc < 2:294472/149‑1 (MQ=21) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGc < 2:68561/149‑1 (MQ=21) cTTGGCACCACAGCAGTACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTa < 3:353303/149‑1 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACa < 8:288281/148‑1 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGAc < 8:209758/149‑1 (MQ=21) aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACCGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGtttt > 7:188376/1‑149 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGtttttt < 8:193144/147‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:92909/141‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCCGTGCATGGCACCCGCTTTTCCCGTGTTAGCGGGCACGCCCCCTACCACAGACAGATTGACATTAAAC‑GGGCCAATAGCTAAAATGAAGGCCCGtttttttagatag > 2:92909/1‑141 (MQ=12) gggAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCCTGGCACCCGCTTTGCCCGTGTTAGCGGGCCCGCCCGCGCCCACAGACTGATTGCCAATAAGC‑GGGCCAAACGCGACAGTGACGGCCCGGTTTTTGTTTCCACGCGAACGa > 8:185758/1‑148 (MQ=16) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 8:73399/1‑149 (MQ=25) gcgggcggGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:35141/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:185758/149‑1 (MQ=25) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCTGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:295726/149‑1 (MQ=21) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc > 1:256231/1‑146 (MQ=25) cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 2:256231/146‑1 (MQ=25) ggCGTGCCAGCGAAACAGGAGCCGCGGTGCATTGCACCGGGTTTGCCGGTGTTCGCGGGCACGCCCGATCCCACAGACTGATTGCCATTAAGA‑GGGGATATCGCTACAGTTCCGGCCCGTTATTGGTTGCCTCGCTAACGAAACaagc > 6:374390/1‑145 (MQ=16) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:276061/148‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttcaggctctgaatggccgcatcacgggcttcagcgttgtc < 1:60928‑M2/148‑105 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCCCAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTGGCCGCGCTAACGAATCATGAGTCCCtt > 7:241059/1‑148 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 4:169044/1‑141 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 3:169044/141‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 8:151569/1‑149 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttca < 6:389687‑M2/109‑68 (MQ=255) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgggcacgcccgctcccaccgtttggcagcctcagtcctgctgtggtgccaaggcttcgttca > 5:389687‑M2/1‑42 (MQ=255) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 7:73399/149‑1 (MQ=37) ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAGTCCCTTAGAACGCTAt > 7:81868/1‑148 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 5:267760/149‑1 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 1:123427/149‑1 (MQ=37) || CGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCC‑TGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGC‑GGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAA > NC_002947/1499342‑1499610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |