Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,811	(T)7→6	intergenic (+48/+705)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,805	0	T	.	100.0%	134.4 / NA	31	intergenic (+42/+711)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (15/16);  total (15/16)

TGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAA  >  NC_002947/4740662‑4740940                                                                                                                                                |                                                                                                                                        tGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCGAGGGCAGCcttctt                                                                                                                                     <  1:73173/148‑1 (MQ=255)     aagaaaTGCTCTCCAGCCCGTACAAGATCGGCAGTCCGCGCCAGCTGTACACCGGCGATCAGAAGCGCGACATCTTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatt                                                                                                                                 <  7:150852/148‑4 (MQ=255)       gaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCttttttatttg                                                                                                                               >  1:21302/1‑143 (MQ=255)                   aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                    <  3:128431/146‑1 (MQ=255)                   aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                  <  3:159631/148‑1 (MQ=255)                                 cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                    <  8:105381/148‑1 (MQ=255)                                   gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                  >  1:14389/1‑148 (MQ=255)                                   gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                  <  1:120130/148‑1 (MQ=255)                                    ccGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCACGCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATAGCGGccattcc                                                                                                 >  7:138155/1‑143 (MQ=255)                                     cGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGACCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGccc                                                                                                >  1:36264/1‑148 (MQ=255)                                          gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                           <  6:108377/148‑1 (MQ=255)                                           cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                         <  7:137037/148‑1 (MQ=38)                                            gtcAGCTGTACACAGGCGAGCAGAAGCGCGTCATCGTTGCCCTGAAAGACCTCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                         <  6:52181/146‑1 (MQ=37)                                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                         <  3:148837/148‑1 (MQ=38)                                            gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                         <  8:34843/148‑1 (MQ=38)                                             ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                        <  7:14310/148‑1 (MQ=38)                                             ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCAGGCCACTTCGCGGCCTTGCCCGCGAagag                                                                                        >  6:48626/1‑148 (MQ=255)                                              cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCCGAgg                                                                                       >  1:53934/1‑148 (MQ=255)                                                 cTGTACACCGGCGAGCAGAAGCGCGACAGCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCACGAAGGCTGCCCTCGGGCAGCC‑GTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGTCCg                                                                                    <  6:84100/148‑1 (MQ=255)                                                                       gctaCATCGTTGCCCTGAAAGACCGAAAGTAAGCTGCAGTAGCCTAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCa                                                              <  8:72506/145‑1 (MQ=255)                                                                          aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtt                                                           >  2:36145/1‑148 (MQ=39)                                                                           cATCGTGGCCCTGAAAGACCGCAAGTAAGCGGCAGAACCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttt                                                          <  7:24062/148‑1 (MQ=255)                                                                                 tGCCCTGAAAGACCGCAAGTACGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                    <  3:160926/148‑1 (MQ=255)                                                                                    ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATAGCGCCCTTTCCCGCGAATAGGCCGGCACAGCAACCACAAAACCTCAGgttttaaacgcc                                                 >  8:44276/1‑141 (MQ=255)                                                                                      tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGCGGCCCGCACCGACCCCACAAAACCTCAGTTTTTCTccgcccg                                               >  6:57717/1‑148 (MQ=255)                                                                                      tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCATCGCCGCCTTGCCCGCGCAGAGGCCGGCACAGACCACACAAAACCTCATGTTTTCTccccccg                                               >  5:6637/1‑148 (MQ=255)                                                                                                        cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGCCCCCACACAACCTCCGTTTTTCTCCGCCCGCTCCCGCCGCCCTTTCAg                             >  4:125048/1‑148 (MQ=255)                                                                                                         tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCGGCCGTGGCCGCGCAGAGGGCGGCCCCGCCCCCCCAAAACCCCAGGTTTTGTCCGCCCGCGCCCGCCCCCCCTTGAgg                            >  1:134639/1‑148 (MQ=255)                                                                                                                gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCGGCCTTGGCCGCGAAGAGGCCGGCACCGCCACCCCAAAACCACAGTTTTTGTCCGCCCCCCCACGCAGCCCGTTACGGGTGttta                     >  4:88253/1‑146 (MQ=255)                                                                                                                       gCAAGACGGCTGCCCTCGGCCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTTCGCGCCCTGGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcgc              <  2:125330/148‑1 (MQ=255)                                                                                                                         aaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGACTTGCCCGCGCAGAGGCCGGCACAGACACCACAAACCCGCAGTTGTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcgcct            >  4:148499/4‑146 (MQ=255)                                                                                                                             agGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTACGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca         >  5:26234/1‑147 (MQ=255)                                                                                                                                 tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATACGCCCGCTCACGCAGCCCTTTAAGGGTATTGAACGGCGCATCcaccac     >  4:67579/1‑147 (MQ=255)                                                                                                                                    ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTGCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa  <  7:72049/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                        TGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAA  >  NC_002947/4740662‑4740940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑