Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,586,035	+G	intergenic (+118/+97)	PP_4061 → / ← PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,586,033	1	.	G	100.0%	32.0 / NA	11	intergenic (+116/+99)	PP_4061/PP_4063	hypothetical protein/long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (3/8);  total (3/8)

AGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGG  >  NC_002947/4585895‑4586098                                                                                                                                            |                                                                    aGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAAcc                                                             >  5:65831/1‑148 (MQ=255)       cACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTc                                                        >  3:107821/1‑147 (MQ=255)         ccGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTTATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTa                                                     <  8:142578/148‑1 (MQ=255)                              aCCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCtt                                <  1:140588/147‑1 (MQ=39)                                cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGaa                             <  2:19481/148‑1 (MQ=39)                                                        tCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGa       <  4:107821/146‑1 (MQ=39)                                                          tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg   >  7:109140/1‑148 (MQ=38)                                                          ggcgcTGCTCCATTGAAAGGGTGAGGATGACCGGGTGCTGGGCAAGGTGAAGAGCTTGCATTGATCGGGCCGGCCTCTTCGCGGGGAAACCCGCTCCTACCAGGGCC‑CGTGCCGGCCGTGAATGCGGCGCTGCCCCGGGTAGGAGCgg   <  1:116221/147‑1 (MQ=255)                                                             gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg  <  6:65831/146‑1 (MQ=38)                                                             gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCATCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCtgg  <  8:109140/146‑1 (MQ=38)                                                             gctgctCCATTGAAAGGTTGAGGATAACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCAGGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCggg  <  8:106372/146‑1 (MQ=38)                                                                                                                                            |                                                                    AGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCAGGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGG  >  NC_002947/4585895‑4586098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑