Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,095,512	+A	coding (549/1494 nt)	rpoN ←	RNA polymerase sigma‑54 factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,095,509	1	.	A	100.0%	100.1 / NA	31	F184F (TTC→TTT)	rpoN	RNA polymerase sigma‑54 factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/20);  total (11/20)

TCCACGGAGTTGTAGCGGGCAGCTGGCGCAGTTGCAGCAACAGGCATTCGCCGAGGTTGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCG‑AAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACTGCCACAGCAGGTGGCTTTGCA  >  NC_002947/1095364‑1095645                                                                                                                                                   |                                                                                                                                         tCCACGGAGTTGTAGCGGGCAGCTGGCGCAGTTGCAGCAACAGGCATTCGCCGAGGTTGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCG‑a                                                                                                                                         >  8:52875/1‑147 (MQ=255)                   gCAGCTGGCGCAGTTGCAGCAACAGGCATTCGCCGAGGTTGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATttctt                                                                                                                       <  4:112734/148‑1 (MQ=255)                        tGGCGCAGTTGCAGCAACAGGCATTCGCCGAGGTTGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAgt                                                                                                                  <  6:125686/148‑1 (MQ=255)                                              aTTCGCCGAGGTTGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGc                                                                                            <  3:156297/148‑1 (MQ=255)                                                   ccGAGGTTGCGGGCGCCTACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGtt                                                                                        <  4:98017/147‑1 (MQ=255)                                                          tGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGt                                                                                <  2:52803/148‑1 (MQ=255)                                                            cGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTc                                                                               <  7:49361/147‑1 (MQ=255)                                                                cgcCAACGCCAGCTGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCa                                                                          <  3:30224/148‑1 (MQ=255)                                                                cgcCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCa                                                                          <  6:5567/148‑1 (MQ=255)                                                                       gCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCAc                                                                   <  5:117157/148‑1 (MQ=255)                                                                       gCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCAc                                                                   >  8:31024/1‑148 (MQ=255)                                                                         cAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACg                                                                  >  4:38570/1‑147 (MQ=255)                                                                           gCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCGTCGACCTCGTCCAGGTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCa                                                               <  4:136589/148‑1 (MQ=255)                                                                              ggCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATc                                                            <  7:78704/148‑1 (MQ=255)                                                                                 tCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAg                                                         >  2:168489/1‑148 (MQ=255)                                                                                    aaCTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGcgg                                                      >  2:112172/1‑148 (MQ=255)                                                                                       tgctgGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGcggtcg                                                   <  6:146098/148‑1 (MQ=255)                                                                                       tgctgGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGcggtcg                                                   >  1:170474/1‑148 (MQ=255)                                                                                       tgctgGATGCGGGGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTGCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGcgggcg                                                   <  6:80995/148‑1 (MQ=255)                                                                                                  gTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACAt                                        <  1:168489/148‑1 (MQ=255)                                                                                                      aGTACCGCTTCGACCTCGTCCAGTGCGATATCCAGCTCCGGGGCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGGCACTGCAATCAAGCGGTCGGTGTCGGACATCGGt                                    <  2:37916/148‑1 (MQ=255)                                                                                                       gTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTg                                   >  3:65833/1‑148 (MQ=255)                                                                                                           cGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGcgg                                                      >  3:159436/1‑125 (MQ=255)                                                                                                           cGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCa                                <  4:149140/147‑1 (MQ=255)                                                                                                            gCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGcggt                                                     <  4:159436/125‑1 (MQ=255)                                                                                                              ttCGAGCTCGTCCATTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCGGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGtt                            <  1:48672/148‑1 (MQ=255)                                                                                                                  aCCTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGCCATCGGTGCCAGGTTCAAc                        >  2:115986/1‑148 (MQ=255)                                                                                                                   ccTCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACt                       >  6:91717/1‑148 (MQ=255)                                                                                                                     tCGTCCAGTTCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACTGc                     >  6:78011/1‑148 (MQ=255)                                                                                                                             ttCGATATCCAGCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACTGCCAcagcag             >  5:510/1‑148 (MQ=255)                                                                                                                                        gCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGCCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACTGCCACAGCAGGTGGCTTTGCa  <  1:73101/148‑1 (MQ=255)                                                                                                                                        gCTCCGGGTCGAAAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGCCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACTGCCACAGCAGGTGGCTTTGCa  <  1:70570/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                         TCCACGGAGTTGTAGCGGGCAGCTGGCGCAGTTGCAGCAACAGGCATTCGCCGAGGTTGCGGGCGCCAACGCCAGCCGGCTCGAACTGCTGGATGCGGTGCAGTACCGCTTCGACCTCGTCCAGTTCGATATCCAGCTCCGGGTCG‑AAACCGGCGCAGATTTCTTCGAGTGTGTCTTCCAGGTAGCCCTGGCCGTTGATGCTGTCGATCAGGGTCACGGCAATCAAGCGGTCGGTGTCGGACATCGGTGCCAGGTTCAACTGCCACAGCAGGTGGCTTTGCA  >  NC_002947/1095364‑1095645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑